Carbohydrate profiling and protein identification of tegumental and excreted/secreted glycoproteins of adult Schistosoma bovis worms (original) (raw)

Identification of Antigenic Glycans from Schistosoma mansoni Using a Shotgun Egg Glycan Microarray

Infection and immunity, 2016

Infection of mammals by the parasitic helminth Schistosoma mansoni induces antibodies to glycan antigens in worms and eggs, but the differential nature of the immune response among infected mammals is poorly understood. To better define these responses we used a shotgun glycomics approach, in which N-glycans from schistosome egg glycoproteins were prepared, derivatized, separated, and used to generate an egg shotgun glycan microarray. This array was interrogated with sera from infected mice, rhesus monkeys, humans, and with glycan-binding proteins and antibodies to gather information about the structures of antigenic glycans, which were also analyzed by mass spectrometry. A major glycan antigen targeted by IgG from different infected species is the FLDNF epitope [Fucα3GalNAcβ4(Fucα3)GlcNAc-R], which is also recognized by the IgG monoclonal F2D2. The FLDNF antigen is expressed by all life stages of the parasite in mammalian hosts and F2D2 can kill schistosomula in vitro in a compleme...