Proteomics of human plasma: A critical comparison of analytical workflows in terms of effort, throughput and outcome (original) (raw)
2013, EuPA Open Proteomics
Blood Biomarker Diagnosis Liquid chromatography Mass spectrometry Shotgun proteomics a b s t r a c t In this study, several workflows to analyze human plasma proteins with RP-LC MS/MS are evaluated. The impact of depletion of abundant proteins on the plasma proteome coverage was assessed together with the duration of RP-LC separation. An additional upstream liquidbased fractionation was evaluated. The applicability and feasibility of these technologies in large-scale clinical studies with respect to effort, throughput, and outcome are discussed.
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