P53 Is Not Mutated In Musocsa Adjacent To Oral Carcinomas (original) (raw)

Abstract

Objectives: p53 is a tumor suppressor gene frequent mutated in oral carcinomas. Clusters of cells overexpressing p53 is often found in adjacent normal mucosa. This apparent up regulation might indicate early malignant changes. The aim of this study is to investigate whether the p53 gene in these clusters is wild-type or mutated. Methods: Tissues from eighteen patients with oral squamous cell carcinoma (OSCC) were snap frozen. All samples, except four, included adjacent histological normal epithelium. p53 positive cells were identified by immunohistochemestry and collected by Laser Capture Microdissection followed DNA extraction. The region between exon 5-9 of the p53 gene was amplified by polymerase chain reaction. Bands showing mutations were further analysed using Denaturering Gradient Gel Electrophoresis . Results: Cell clusters in normal epithelium overexpressing p53 was seen in 13 out of 14 cases. The positive staining was located to nuclei confined to the basal and parabasal l...

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