HPTLC method for analysis of piperine in fruits of Piper species (original) (raw)
Related papers
HPTLC method for analysis of piperine in fruits ofPiperspecies
Journal of Planar Chromatography – Modern TLC, 2011
Piperine, an alkaloid with diverse biological activity commonly occurring in fruits of Piper sp., has high commercial, economical, and medicinal value. In this communication densitometric estimation of piperine from fruits of Piper nigrum L., processed Piper nigrum L. (white pepper), Piper longum L., Piper retrofractum Vahl, Piper cubeba Hunter, and Piper betle L. is reported. Extracts of these fruits and a standard solution of piperine were applied to silica gel F 254 HPTLC plates and the plates were developed in a twin-trough chamber with toluene-ethyl acetate-diethyl ether 6:3:1 as mobile phase. The plates were scanned at 337 nm and quantification of piperine was based on a predetermined calibration plot obtained by chromatography of 15 to 75 ng standard. The quantity of piperine was highest in fruits of Piper nigrum L. and least in Piper betle L. The trend was Piper nigrum L. > Piper longum L. > processed Piper nigrum L. > Piper retrofractum Vahl > Piper cubeba Hunter > Piper betle L. The HPTLC method was found to enable simple, convenient, rapid screening and quantification of the active marker piperine in six fruits of Piper sp. commonly used in the Indian traditional system of medicine. 2 Experimental 2.1 Plant Material, Reagents, and Solutions Fruits of Piper nigrum (black pepper) and Piper longum, were collected from Dapoli, Maharashtra, India, and fruits of Piper betle were collected from Vir, Satara, Maharashtra, India during early 2008. Piper nigrum (white pepper), Piper retrofractum, and Piper cubeba were purchased from the crude drug market of Pune, Maharashtra, India. These fruits were authenticated and deposited in the crude drug repository of Agharkar Research Institute, Pune 411 004, India (voucher specimen numbers: Piper nigrum L.-F133, processed Piper nigrum L.-F132, Piper longum L.-F134, Piper retrofractum Vahl-F135, Piper cubeba Hunter-F136, and Piper betle L.-F137). Piperine standard was procured from Sigma-Aldrich (Steinheim, Germany; ≥98.0%). Other analytical grade solvents and reagents were purchased from SD Fine Chemicals, Mumbai, India. A stock solution of piperine (1 mg mL-1) was prepared by dissolving 10 mg, accurately weighted, in methanol and diluting to 10 mL. Stock solution (0.050 mL) was transferred to a volumetric flask and diluted to 10 mL with methanol to furnish working standard solution.
Pharmacology & Pharmacy, 2017
In the pharmaceutical world the majority of the active pharmaceutical ingredients (API) have been obtained from the natural products. Piperine is such naturally occurring alkaloid which can be considered as major bioactive phytochemical having broad spectrum of pharmacological activities. It is obtained from the most valuable ethnomedicinal spices peppercorns i.e. black pepper and white pepper, which are the fruits of the Asian vine Piper nigrum L. Because of the widespread traditional uses of this medicinal compound, present article reveals a simple and effective isolation method of bulk piperine. The novelty of this investigation is to provide an idea for utilizing such natural method of large scale commercial piperine production as API drug in spite of chemical synthesis. Piperine was isolated in a pure crystal form and characterized by its melting point, X-Ray diffraction (XRD) studies and spectral data, including two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Chromatographic techniques like Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were applied to determine the purity of the yielded piperine. It was found that piperine yield from black pepper was within 2.5%-3.0% and from white pepper within 4.0%-4.5% and the purity of the yielded piperine was found to be up to 98.5% for black pepper and 98.2% for white pepper. Considering this yield value and purity it is indicated that, such effective isolation method can be successfully utilized for industrial large-scale production commercially. According to the result, it can be claimed that, as a natural product the isolated piperine can also be utilized as API drug like other expensive chemically synthesized piperine in different drug formulation.
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram
One of the most famous spice species that lives in the tropics is black pepper (Piper nigrum L.). The study of piperine paved the way for its development as a bioactive compound in the treatment of various diseases, this is based on the ancient healing tradition of using black pepper. Our current study aims to isolate and characterize the bioactive compound piperine in Piper nigrum L. Black pepper was extracted with ethanol using the basic soxhletation and percolation methods. The results in the study showed that the extract from the Sokhlet process was yellowish moss green, and the piperine crystal color was yellow and shaped like a needle. Thin layer chromatography (TLC) results were observed using a UV-Vis spectrophotometer with different eluents and different Rf values were obtained. In addition, observations on the IR spectrum show that the piperine structure has been formed. This study provides an opportunity for a more in-depth exploration of the structure of piperine and fut...
Pharmacology & Pharmacy
In the pharmaceutical world the majority of the active pharmaceutical ingredients (API) have been obtained from the natural products. Piperine is such naturally occurring alkaloid which can be considered as major bioactive phytochemical having broad spectrum of pharmacological activities. It is obtained from the most valuable ethnomedicinal spices peppercorns i.e. black pepper and white pepper, which are the fruits of the Asian vine Piper nigrum L. Because of the widespread traditional uses of this medicinal compound, present article reveals a simple and effective isolation method of bulk piperine. The novelty of this investigation is to provide an idea for utilizing such natural method of large scale commercial piperine production as API drug in spite of chemical synthesis. Piperine was isolated in a pure crystal form and characterized by its melting point, X-Ray diffraction (XRD) studies and spectral data, including two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy. Chromatographic techniques like Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were applied to determine the purity of the yielded piperine. It was found that piperine yield from black pepper was within 2.5%-3.0% and from white pepper within 4.0%-4.5% and the purity of the yielded piperine was found to be up to 98.5% for black pepper and 98.2% for white pepper. Considering this yield value and purity it is indicated that, such effective isolation method can be successfully utilized for industrial large-scale production commercially. According to the result, it can be claimed that, as a natural product the isolated piperine can also be utilized as API drug like other expensive chemically synthesized piperine in different drug formulation.
Quantification of Piperine from Single herb formulation containing Piper nigrum by HPTLC
2016
Background: Black pepper is the important plant in Ayurveda possessing Immunomodulator property. Objectives: To develop HPTLC method for Piperine from single herb formulation. Settings and Design: A HPTLC method was developed for the determination of Piperine in Single herb formulation. Materials Methods: Analysis of Piperine was performed on HPTLC aluminium plates pre-coated with silica gel 60F254 as a stationary phase. Ascending development was carried out in twin trough glass chamber previously saturated with mobile phase consisting of toluene: ethyl acetate: formic acid (8: 3: 0.5 v/v/v) at room temperature (25±2ºC). The densitometric scanning was carried out at wavelength 340 nm by using Camag TLC scanner-3. Statistical Analysis: The RSD and Correlation coefficient (r 2) were calculated by standard formulas Results: Piperine was appeared in chromatographic zones at Rf value 0.52 for Single herb formulation. The linear calibration plots showed good results with r 2 = 0.998. Conc...
Pharmacognostical and Phytochemical study of Piper longum L. and Piper retrofractum Vahl
Piperine, an alkaloid with diverse biological activity commonly occurring in fruits of Piper sp., has high commercial, economical, and medicinal value. In this study densitometric estimation of piperine from fruits of Piper longum L and Piper retrofractum Vahl.. It can be stated from this study that at industrial level P. longum Lin. is more suitable because of its preservation less and high percentage of Piperine(3.26µg/ml) than P.retrofractum Vahl.(0.49µg/ml) pharmacognostical and phytochemical characters of both species is discussed in present study.
Pharmacognostical and Chromatographic Analysis of Malaysian Piper nigrum Linn
2016
Dried Piper nigrum Linn. fruit, also known as black pepper, is an economically valuable medicinal plant that has diverse pharmacological activities. The current study aimed to develop an analytical profile of P. nigrum fruits that can be used as a tool for quality control and standardisation of this plant in Malaysia. Black pepper specimens were collected from three different locations in Malaysia; namely Johor, Malacca and Sarawak. Various tests were conducted in the present work to obtain detailed pharmacognosy and chromatographic profiles of P. nigrum fruits, including plant morphology, taxonomy characteristics, phytochemical screening, physicochemical properties and chromatographic analysis. Using a validated high performance liquid chromatography method we quantified piperine in the samples and the highest content was obtained for the specimen from Malacca. It can be concluded that the proposed analytical methods could be applied for both qualitative and quantitative analysis o...
2011
Piperine, a characterizing compound present in fruits of Piper nigrum and Piper longum used as bioavailability enhancer. Ingredients of antioxidant, anti-inflammatory activity has been extracted using soxhlet and supercritical fluid extraction technique. It was isolated using column chromatography. Characterization of compound was done by spectroscopic technique. A simple, rapid, accurate and specific HPTLC method developed and validated. The method proposed can be used for the routine analysis of both P.nigrum and P.longum fruit material and its formulations.
Analysis for piperine in leaves, roots and spikes in Piper longum L
2018
Piper longum L (Pipali) is an important medicinal plant used as an analgesic, antipyretic, CNS depressant, anti-inflammatory, anti-tumor, sedative, hypnotic, tranquilizer and muscle relaxant, hepatoprotective both in digestive and respiratory systems. The fruit of Piper longum contains a large number of alkaloids and related compounds, among them the most important is piperine. Attempts were made to detect piperine in different parts of the Piper longum plant. Leaf and root of both male and female plants and spike from only female plant were used. Methanol extracts of above mentioned parts were prepared and subjected to thin layer chromatography (TLC) along with piperine as a standard, using toluene-ethyl acetate (70:30) mobile phase. Upon treatment with vanillin-sulphuric acid reagent and subsequent heating the silica gels and viewed under UV light. Appearance of yellow fluorescence was observed in all the samples except the male leaf. This confirmed the presence of piperine in all...