Determination of HCV Genotypes, in Iran by PCR-RFLP (original) (raw)
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Determination of hepatitis C virus genotypes among HCV positive patients in Shahrekord, Iran
African Journal of Microbiology Research, 2011
Hepatitis C is one of the most common causes of the liver failure and cancer and represents a major public health problem. Recent studies have focused on whether different hepatitis C virus (HCV) genotypes, are associated with different profiles of pathogenicity, infectivity and response to antiviral therapy. Genotyping system based on polymerase chain reaction (PCR) of the core region with genotype-specific PCR primers for the determination of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a was developed. Different genotypes have been reported in different parts of the world. Genotype 1 is difficult to treat, while genotypes 2 and 3 are easy to treat. Therefore, identification of HCV genotype in patients is necessary to begin and follow up the treatment. In this study, viral genomic of 94 patients extracted from sera were detected by nested-real time (RT) PCR. PCR products were digested with proper enzymes and studied by restriction fragment length polymorphism (RFLP). The results of PCR-RFLP were as follows: 1a (54.26%), 1b (11.71%), 3a (27.66%), 2a (2.12%) and 4 (4.25%). This indicates that a high percentage of HCV infected patients.
Molecular epidemiology of hepatitis C virus genotypes in Bushehr province, Iran
European Review for Medical and Pharmacological Sciences, 2010
Background and Objectives: Molecular epidemiology of hepatitis C virus (HCV) is very important for the treatment of hepatitis C infection. The aim of this study was to determine the distribution of HCV genotypes in Bushehr province (South West of Iran). Materials and Methods: A total of 100 patients who were detected as positive for HCV antibody (by using ELISA method and RIBA test) referred to Arya Virology Laboratory between 2007-2009 in order to molecular diagnosis and furthermore virus genotyping. After detection of HCV, RNA genotyping of virus was done by using genotype specific primers. Results: Genotype 1a was found in 49% of the patients and genotype 3a was found in 40% of the patients and 1b in 5% of patients, while the genotype of the virus could not be identified in 5% of the patients. Finally, in 1% of patients coinfection due to 1a-3a genotypes was identified. Conclusion: The dominant genotype of HCV in Bushehr province, Iran, was determined as 1a.
Genotypic determination of Hepatitis C virus in Tehran using PCR-RFLP analysis
Gene Therapy and …, 2007
is the etiologic agent of most parentally transmitted hepatitis viruses and is responsible for more than 60% of chronic hepatitis cases that lead to liver transplantation. Based on its genetic variability, HCV is classified into at least six genotypes and a series of subtypes. HCV genotyping is important in order to select appropriate therapy. The aim of this study was to determine which HCV virus genotypes are most prevalent in Tehran. Serum HCV RNA was extracted by RNX plus buffer. RT-PCR and nested PCR were used on the 5' untranslated region and a 250 bp fragment was amplified. 80 HCV positive sera samples were selectively chosen from 250 patients who were referred to the laboratory by their physicians. HCV genotypes were determined using restriction fragment length polymorphism (RFLP). The patients were grouped as follows: 32 cases (40%) had type 1a, 15 cases (18.75%) had type 3a, 7 cases (8.75%) had type 1b, 5 cases (6.25%) had type 3b, 3 cases (3.75%) had type 4, and 18 cases (22.5%) could not be typed because their sequences differed from any that were previously reported. Our results showed that types 1a and 3a were the most prevalent HCV genotypes in our Tehran samples, while types 2a, 2b, 5, and 6 were not observed.
Hepatitis C virus (HCV) is the major cause of chronic liver disease such as acute and chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. This virus is responsible for more than 60% of chronic hepatitis. Based on its genetic variability, HCV is classified into at least six genotypes and a series of subtypes (1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a), while Genotype one is difficult to treat but genotypes 2 and 3 are easy to treat. Different genotypes are seen in different parts of the world. HCV genotyping is important for prediction of success of chemotherapy and progression of liver diseases. So recent studies have focused on determination of HCV genotypes. In this study, viral genomic of 86 patients (from different laboratories of Esfahan) extracted from sera were detected by nested-real time (RT) PCR. PCR products were digested with proper enzymes and studied by restriction fragment length polymorphism (RFLP). The results of PCR-RFLP were as follows: 1a (52.3%), 1b (11.6%), 3a (29.1%), 2a (2.3%), 4 (4.7%). Our results showed that types 1a and 3a were the most prevalent HCV genotypes in our samples. The results of this study will guide clinicians in successful monitoring and treatment of HCV patients in our region.
Virology Journal, 2010
Background: Hepatitis C is an infectious disease affecting the liver, caused by the hepatitis C virus (HCV). The hepatitis C virus is a small, enveloped, single-stranded, positive sense RNA virus with a large genetic heterogeneity. Isolates have been classified into at least eleven major genotypes, based on a nucleotide sequence divergence of 30-35%. Genotypes 1, 2 and 3 circulate around the world, while other genotypes are mainly restricted to determined geographical areas. Genotype determination of HCV is clinically valuable as it provides important information which can be used to determine the type and duration of therapy and to predict the outcome of the disease. Results: Plasma samples were collected from ninety seven HCV RNA positive patients admitted to two large medical laboratory centers in Isfahan province (Iran) from the years 2007 to 2009. Samples from patients were subjected to HCV genotype determination using a PCR based genotyping kit. The frequency of HCV genotypes was determined as follows: genotype 3a (61.2%), genotype 1a (29.5%), genotype 1b (5.1%), genotype 2 (2%) and mixed genotypes of 1a+3a (2%). Conclusion: Genotype 3a is the most frequent followed by the genotype 1a, genotype 1b and genotype 2 in Isfahan province, Iran.
Distribution of Hepatitis C virus genotypes in city of Mashhad, North-east of Iran
Indian Journal of Medical Microbiology, 2014
Background: Chronic hepatitis C virus (HCV) infection is the major cause of liver disease related morbidity and mortality in hemophilic patients who needs regular blood product administration. Although genotype of infecting HCV is one of the prime predictors of response to antiviral therapy however, its distribution in hemophilic patients is still unclear and just few studies with low sample sizes have investigated this issue. Therefore, in this study, we aimed to identify this distribution in 367 Iranian hemophilic patients.
Genotype distribution of Hepatitis C Virus in Khorasan Razavi Province, Iran
Turkish Journal of Medical Sciences, 2013
Background/aim: Several types of the hepatitis C virus (HCV), with variations in different parts of the genome, have been isolated from different regions of the world. Based on heterogenic sequences in the isolated genome, HCV is classified into different genotypes and subtypes. Data on distribution of HCV genotypes in a certain region could be important to patient management. Therefore, this study was conducted to determine the distribution of HCV in Mashhad, Northeast Iran. Materials and methods: This cross-sectional study was conducted on 103 patients with HCV infections in Mashhad. Among the participants, at least 22 (21.4%) were intravenous drug users. HCV seropositivity was determined by an enzyme-linked immunosorbent assay and was confirmed by reverse transcriptase polymerase chain reaction. HCV-positive samples were selected for HCV genotyping using genotype specific primers. Results: Of 103 subjects, 43 (41.7%) and 34 (33.0%) had genotypes 1a and 3a, respectively. Other genotypes including 1b, 2a, 2b, 3b, and 5a were found in 4 (3.9%), 1 (1.0%), 3 (2.9%), 4 (3.9%), and 1 (1.0%), respectively. Coinfections with 2 genotypes were also observed in 11 (10.7%) patients. Genotyping for 2 (1.9%) of 103 samples did not produce any results. Conclusion: Genotypes 1a and 3a were found to be the most prevalent HCV genotypes in Mashhad, Iran.
Hepatitis C Virus Genotypes and Association With Viral Load in Yazd, Central Province of Iran
Hepatitis Monthly, 2014
Background: Hepatitis C virus (HCV) is a major cause of liver disease. Infection with HCV is a global public health problem. The virus is classified into 6 genotypes and more than 80 subtypes named as a, b, c, etc. HCV genotyping has been an important parameter for the treatment of HCV infection. Objectives: The main aim of this study was to estimate the prevalence of HCV genotypes in Yazd, central province of Iran. In addition, the study investigated whether there was any association between HCV load and genotypes. Patients and Methods: This descriptive cross-sectional study was performed on samples suspicious of HCV infection from March 2010 to June 2013. Peripheral blood sample was obtained and screened for anti-HCV antibodies using Enzyme-Linked Immunosorbent Assay (ELISA). Then sera of anti-HCV positive samples were analyzed using quantitative polymerase chain reaction method. Plasma samples were used to determine the HCV genotypes of 1a, 1b, 2, 3, and 4 in 191 infected patients. Results: One hundred fifty-two out of 191 (79.6%) samples were from male patients. The mean of the patients' age was 40.7 ± 11.9 years (range 21-75 years old). Sixty-three (33%) patients were included in 31-40 years group. The mean number of HCV in infected patients was 2.92 × 10 6 ± 1.85 × 10 6 copies/mL (Min: 508; Max: 2.75 × 10 8 copies/mL). HCV genotype 3 was the predominant genotype (50.3%) followed by subtypes 1a (38.7%) and 1b (6.8%). The distribution of other HCV genotypes showed genotype 2 in 1.6% and mixed genotypes in 2.6% of positive samples. Genotype 3 was predominant in all age groups except 21-30 years of age group. We were unable to find any significant difference between mean viral load of the patients infected with genotype 3 and those infected with genotype 1 (1a and 1b). Conclusions: Findings of the present study showed that HCV genotype 3 was the predominant genotype followed by the subtypes 1a and 1b in Yazd, central province of Iran. In addition, there was no difference between HCV load and genotypes 1 and 3. HCV genotyping is recommended in other provinces of Iran.
Genotypic analysis of hepatitis C virus in khuzestan province, southwestern iran
Middle East journal of digestive diseases, 2011
BACKGROUND Hepatitis C virus (HCV) infection is responsible for considerable morbidity and mortality worldwide. The HCV genotype has a geographic distribution and an important role in clinical and histological outcomes. This study determined HCV genotypes and their related risk factors among patients from Khuzestan Province, Southwest Iran. METHODS In a cross-sectional study, 223 patients infected with HCV who referred to Ahwaz Jundishapour University Hospitals (AJSUH) and Hepatitis Clinic were enrolled. Specific and nested polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLPs) were performed to determine viral infection and genotype analysis. Liver enzymes including ALT and AST and the correlated risk factors were also determined. genotype 1a (41.7%); genotype 1b (2.7%); genotype 2 (4.1%); genotype 3a (31.4%); and genotype 4 (1.8%). There were 42 samples (18.84%) not classified into any of the known HCV subtypes. No patient was infected with more than...
Hepatitis C virus genotype distribution in Shiraz, southern Iran
2009
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