Cryptic diversity within two endemic crayfish species of the Southeastern US revealed by molecular genetics and geometric morphometrics (original) (raw)
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The future of endangered crayfish in light of protected areas and habitat fragmentation
Scientific Reports
The long-term survival of a species requires, among other things, gene flow between populations. Approaches for the evaluation of fragmentation in the frame of freshwater habitats consider only a small amount of the information that combined demography and geography are currently able to provide. This study addresses two species of Austropotamobius crayfish in the light of population genetics, spatial ecology and protected areas of the Carpathians. Advancing the classical approaches, we defined ecological distances upon the rasterised river network as a surrogate of habitat resistance to migration, quantifying the deviations from the species´ suitability range for a set of relevant geospatial variables in each cell of the network. Molecular analyses revealed the populations of the two Austropotamobius crayfish species are clearly distinct, lacking hybridisation. Comparing pairs of populations, we found, in some cases, a strong disagreement regarding genetic and ecological distances,...
Biodiversity and Conservation, 2014
The degradation of aquatic habitats has increasingly become one of the most important factors influencing the distribution of freshwater species worldwide. We analysed the occurrence of three crayfish species, Astacus astacus, Austropotamobius torrentium and Orconectes limosus, in relation to habitat degradation status (based on Directive 2000/60/ES), stream morphology, geographical characteristics and land cover. In total, we analysed 6,768 sites within the Czech Republic (Central Europe), of which 6,187 sites lacked crayfish; among the remainder, A. astacus was present in 507 sites, O. limosus occurred in 44 sites and A. torrentium was present in only 30 sites. The analysis revealed that A. astacus preferred streams of better water quality that were not surrounded by agricultural land or settlements. This species also preferentially occurred in smaller streams with stony bottom substrata that were located at higher altitudes. Austropotamobius torrentium occurrence was associated with the natural character of the water body and the presence of protected areas at higher altitudes. Conversely, the non-native crayfish species O. limosus was typically recorded at lower altitudes in downstream reaches surrounded by agricultural land and with deteriorated water quality. The degree of environmental (human) Communicated by pressure besides differences in habitat characteristics at sites with ICS and NICS may be of general importance for conservation strategies aimed at central European native crayfish species, primarily because the habitat-driven co-occurrence pattern (and its possible changes in the future) may strongly influence interspecific relationships, such as direct competition and the spread of infectious diseases between crayfish species.
Conservation phylogenetics of Ozark crayfishes: Assigning priorities for aquatic habitat protection
Biological Conservation - BIOL CONSERV, 1998
A molecular phylogeny based on nucleotide sequence data from the 16S region of the mitochondrial genome was estimated for 20 species of crayfish native to the Ozark Plateaus region of Missouri and Arkansas, USA. With this phylogeny and information on geographic distribution, ecological specialization, and species abundance, species were assessed for their status as rare and endangered. Three species not previously listed, Orconectes nana, Orconectes macrus, and Cambarus maculatus, merit inclusion as ‘rare’ in the Rare and Endangered Species of Missouri Checklist. Three other species merit inclusion with the status ‘Watch List’: Orconectes hylas, Orconectes medius, and Cambarus hubbsi. Additionally, the status of the following species merit changing from ‘Watch List’ to ‘Rare’: Orconectes peruncus and Orconectes quadruncus. Faunal regions within the Ozark Plateaus were ranked for conservation priority using species richness, taxonomic diversity, and phylogenetic diversity. The White ...
1. Populations of white-clawed crayfish (Austropotamobius pallipes) have undergone substantial declines across Europe. Remaining populations tend to be fragmented and in many catchments they are restricted to upland streams. Information is needed concerning their spatial ecology to assist with conservation and rehabilitation of existing fragmented populations, as well as possible reintroductions. 2. A novel method for the long-term tagging of white-clawed crayfish was used to study the spatial ecology of a white-clawed crayfish population fragment in a small, moderate-gradient upland stream. Internal passive integrated transponder (PIT) tags enabled adult crayfish (carapace length > 27 mm) to be permanently tagged, each with a unique identification code and resulted in a high number of recaptures. Of 501 crayfish tagged 413 were subsequently relocated at least once. 3. Crayfish did not make extensive movements, the median annual distance moved was 84.8 m yr À1 , equivalent to annual net movement of 0.233 m day À1 , substantially less than reported in previous studies. The lower levels of movement may reflect the study encompassing all seasons, including winter, when crayfish are relatively inactive. 4. Significantly more crayfish moved downstream compared with upstream and distances moved downstream were significantly greater than those in an upstream direction. This may be linked to the relatively high gradient of the stream and a reduced passability of the abundant riffles to upstream movements of crayfish. 5. A small weir acted as a barrier within the stream, preventing upstream movements of crayfish. Even small barriers may limit the movements of white-clawed crayfish and have the potential to limit connectivity within populations and prevent expansion or recolonization. 6. The results presented suggest that fragmented populations of white-clawed crayfish in upland streams are unlikely to expand rapidly and reconnect to other population fragments, even where habitat is suitable. In establishing stream populations care should be taken to ensure that even small barriers to movement are removed unless these are intended to exclude non-native biota, especially crayfish.
SUMMARY 1. Dispersal and habitat use are key elements in determining impacts of introduced species. We examined if an invasive crayfish species showed a different pattern of movement, dispersal and refuge use to that of a species that it displaces. 2. Fifteen adults of the introduced American signal crayfish, Pacifastacus leniusculus and 20 adult native white-clawed crayfish Austropotamobius pallipes were radiotagged in an area of river where in 2003 they co-occurred and the spatial behaviour and habitat use of the two species were examined. Subsequent surveys at the study site in 2005 recorded no A. pallipes and the density of P. leniusculus had increased. 3. Clear differences in the spatial behaviour of the two species were found. The median distance moved per day by signal crayfish was over twice that of white-clawed crayfish, and dispersal from release locations was higher in signal crayfish. A similar range of refuges with a high degree of overlap was used by both species suggesting that the potential for competitive interaction between the two species exists. 4. The greater movement and dispersal by signal crayfish recorded here supports the hypothesis that invaders are better dispersers than the species that they displace. In addition, movements by signal crayfish may allow them to make better use of patchy resources than white-clawed crayfish and this, coupled with similar microhabitat use, may contribute to the observed replacement.
Conservation Genetics, 2009
We investigated the endangered status and taxonomic status of the freshwater crayfish Procambarus ferrugineus, a crayfish species considered for the candidate list of the Endangered Species Act. This species has a narrow distribution from central Arkansas, USA and is codistributed with its presumed sister species, Procambarus liberorum. We sampled extensively throughout the ranges of both primary burrowing species and collected mitochondrial DNA from a hypervariable fragment of the 16S gene from 109 individuals across 22 sites. We also collected data from a variable region of the 12S gene from a subset of the resulting 16S haplotypes. Due to our inability to sample what we considered P. ferrugineus in the field, we included museum specimens from the United States Natural History Museum of both P. ferrugineus and P. liberorum. Analyses of the resulting data suggested that these two species are indeed the same and we therefore synonymize them under the name of priority—P. liberorum. Additionally, our sampling discovered three new cryptic species from southwestern Arkansas all from the genus Procambarus. Nested clade phylogeographic analysis coupled with population genetic analyses suggested that P. liberorum has had three rounds of range expansion throughout the inferred evolutionary history. Using IUCN Red List criteria for conservation assessment, we conclude that the species P. liberorum should be considered stable, but with special concern because of habitat fragmentation and urbanization, small restricted range, and a moderate level of genetic diversity. Procambarus reimeri should be considered endangered due to its limited geographic range and the potential for a decline in suitable habitat. The three potentially newly discovered species should be considered data deficient until more information is obtained on their distributional limits and habitat requirements. Our study highlights the importance of thorough geographic and taxonomic sampling coupled with the utility of collecting data from museum specimens to reach robust taxonomic and conservation conclusions for endangered species.