TAXONOMIC STUDY OF TEA CULTIVARS, CALTIVATED IN PAKISTAN BY USING MORPHOGENETIC APPROACH (original) (raw)
Related papers
Genetic structure and diversity of India hybrid tea
The most important evolutionary event in the success of commercial tea cultivation outside China in *30 countries came about by the origin of India hybrid tea in India, derived from the extensive spontaneous hybridization that took place between the Assam type tea growing in the forest regions of Assam, North-East India and China type tea introduced from China in *1875 to many regions of North-East India. The release of an enormous pool of vigorous and highly variable plants of India hybrid tea in North-East India was a significant step forward for the origin and evolution of tea as a highly successful crop plant. The 1,644 accessions and clones of India hybrid tea, representatives of known 15 morphotypes, were screened by 412 AFLP markers amplified by 7 AFLP primer pair combinations. All the 412 genetic loci were polymorphic across the 1,644 accessions and clones. The analysis was done with distance (PCoAand NJ) methods, and the STRUCTURE (Bayesian) model. Both PCoA and NJ analysis clustered 1,644 tea accessions and clones into six major groups with one group in each, constituted mostly by China hybrid, Assam China hybrid and Assam hybrid morphotypes, of distinct genetic identity.No group was exclusive for any particular morphotype. The accessions and clones belonging to morphotypes, Assam type, Assam hybrid, China hybrid and China Cambod were distributed in all the groups. It is the Assam type/ Assam hybrid morphotypes which exhibit much broader genetic variability than in China type/China hybrid/Cambod type/Cambod hybrid morphotypes. The STRUCTURE analysis inferred 16 populations (K = 16), for which the greatest values of probability were obtained.Nine of the16 clusterswere constituted by the tea accessions and clones of ‘pure’ ancestry. The remaining clusters were of ‘mixed’ ancestry. This analysis provides evidence that the accessions and clones of the same morphotype are not always of same genetic ancestry structure. The tea accessions and clones obtained from outside North-East India shared the same groups (distance method) and clusters (STRUCTURE model) which were constituted by North-East India accessions. The present study also demonstrates very narrow genetic diversity in the commercial tea clones vis-a `-vis the profound genetic diversity existing in the tea accessions. These clones were distributed in hardly two of the six groups in NJ tree. The identified 105 core accessions and clones, capturing 98% diversity, have their origin from almost all groups/subgroups of NJ tree.
Pakistan Journal of Agricultural Research, 2021
T ea (Camellia sinensis L.) is one of the important healthy beverages used globally and being cultivated in more than thirty countries in different tropical and subtropical zones of the World (Adnan et al., 2013) having above eighty two genetically distinct species (Sultana et al., 2008). Tea cultivation contributes manifolds in improving the economy and job opportunities status in various Asian and African Abstract | The present study was conducted at the tea nursery farm of PARC-National Tea and High Value Crops Research Institute, Shinkiari, Mansehra, Pakistan during the years 2014-16 to assess genetic variability, heritability and genetic advance as percent of mean for various growth attributing traits of fifteen early flushing tea (Camellia sinensis L) clones. The experiment was laid out in randomized complete block design (RCBD) with three replication and 100 plants per replication. Data were recorded on plant height (PH), number of branches per plant (NBP), number of leaves per plant (NLP), stem diameter (SD), main root length (MRL), root diameter (RD), number of lateral roots per plant (NLRP), dry shoot weight (DRW) and dry root weight (DRW). The highest plant height (78.66 cm) was produced by clone BP 2-2, followed by Clones BP 1-4, BP 1-3, BP 3-1 and BP 2-5 with plant height of 70.46, 67.60, 67.20 and 66.36 cm respectively. Clone BP 2-2 also produced highest number of leaves per plant (34.66), stem diameter (6.53 mm), root length (35.10 cm), root diameter (6.55 mm), number of lateral roots per plant (6.35), Dry shoot weight (17.56 g) and dry root weight (11.57 g). Number of branches per plant in the early flushing tea clones ranged from 1.33 (BP 2-4) to 6.00 (BP 6-1). The phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV), but the difference between PCV and GCV was minimal for most of the traits revealing little influence of the environment in the expression of these traits.
Biochemical Genetics, 2008
To revive cultivation of the tea unique to the western Himalayan region, it is important to evaluate the seed-derived bushes available in the area’s abandoned gardens. This study used quantitative leaf characters, catechin content, and AFLP markers to assess these China cultivar type bushes. Compared with other China cultivar germplasm, these accessions showed a higher level of diversity among themselves. Among the quantitative morphological characters, leaf length is important in distinguishing the accessions studied, with a high loading value in the principal component analysis. The catechins and AFLP markers displayed the genetic makeup of the accessions. Other than total catechins, the trihydroxylated catechins showed a high loading value in differentiating the accessions. The genetic control of the ratio of dihydroxylated and trihydroxylated catechins is found to be based on a correlation with AFLP markers. The genetic similarity between Kangra Asha and Kangra Jat suggests that Kangra Jat must be descended from Kangra Asha. Kangra Jat is well adapted to local environmental conditions, as is evident from its high catechin content.
Variability analysis of some genotypes in Nigeria tea (Camellia sinensis) germplasm
World Journal of Advanced Research and Reviews, 2021
Thirty four tea clones were sourced from Cocoa Research Institute of Nigeria tea germplasm and raised through stem cuttings for 10 months in the nursery. The experiment was laid out in a randomized complete block design (RCBD) with 3 replications in 2016. Agronomic and yield data were collected and subjected to analysis of variance. Single linkage cluster analysis (SLCA), principal component analysis (PCA) and FATCLUS analysis were employed to analyse the data. ANOVA showed considerable significant variation p<0.05 among the 34 tea genotypes. The PCA showed that Plant Height (PH) 0.39, Number of Leaves (NL) 0.38, Number of Branches (NB) 0.37, Harvestable Points (HP) 0.31, Stem Diameter 0.39 and Leaf Breadth 0.30 accounted for most of the variations observed. Axes 1, 2 and 3 of the PCA accounted for 37.23%, 15.48% and 10.75% variability respectively with cumulative value of 63.47%. The genotypes were clustered into 7 groups by FASTCLUS Analysis. The dendrogram showed that the firs...
Genetic base of tea (Camellia sinensis L.) cultivars in Sri Lanka as revealed by pedigree analysis
Journal of Applied Genetics, 2007
An understanding of genetic diversity and relationships among breeding materials is a prerequisite for crop improvement. Coefficient of parentage (COP) can be used to measure the genetic diversity among genotypes on the basis of pedigree information. In the present study, COP was estimated for 56 cultivars, including commercial tea cultivars developed by the Tea Research Institute of Sri Lanka and their parental lines. Mean COP of the 56 accessions studied was 0.097 and the value was raised up to 0.272 when non-related pair-wise comparisons were excluded. A single cultivar (Assam/Cambod introduction) was the nucleus of the commercial cultivars. Group mean COP of the cultivars derived from Assam/Cambod parentage was 0.17. Thirty-three percent of the pair-wise comparisons had 0.00 COP, highlighting that many cultivars were unrelated. Within the pedigree, 2 major COP clusters were identified: Assam/Cambod open-pollinated half-sib progenies, and full-sib progenies derived from crosses between Assam/Cambod and other parental lines. The elite groups within the pedigree, where Assam/Cambod parentage was concentrated, were also identified. Information generated in this study should be useful for effective utilization of available diversity in future breeding programmes as well as for proper conservation of genetic diversity in the adapted germplasm. This is the first report on estimates of genetic diversity based on COP in a woody perennial crop, such as tea.
TAG Theoretical and Applied Genetics, 1997
AFLP markers were successfully employed to detect diversity and genetic differentiation among Indian and Kenyan populations of tea (Camellia sinensis (L.) O. Kuntze). Shannon's index of diversity was used to partition the total phenotypic variation into between and within population components. On average, most of the diversity was detected within populations, with 79% of the variation being within and 21% being between populations of Indian and Kenyan tea. A dendrogram constructed on the basis of band sharing distinctly separated the three populations of tea into China type (sinensis), Assam type (assamica) and Cambod type (assamica ssp. lasiocalyx) in a manner consistent with the present taxonomy of tea, the known pedigree of some of the genotypes and their geographical origin. Principal coordinate (PCO) analysis grouped Assam genotypes both from India and Kenya supporting the suggestion that the Kenyan clones have been derived from collections made in this region. The China types were more dispersed on the PCO plot which is a reflection of wider genetic variation. As would be expected, clones collected from the same region exhibited less overall genetic variation. AFLP analysis discriminated all of the tested genotypes from India and Kenya, even those which cannot be distinguished on the basis of morphological and phenotypic traits.
Assessment of Genetic Relationship and Hybrid Evaluation Studies in Tea (Camellia sp.) by RAPD
The genetic relationships among 12 tea accessions representing three species in the genus Camellia were studied using random amplified polymorphic DNA (RAPD) markers. The genetic distance matrix based on Euclidian Distances showed a minimum genetic distance of 2.24 between 'UPASI-2' and 'UPASI-3' clones and the maximum was 4.47 between 'TRF-1' and 'TRI-2025'. The dendrogram based on Ward's method of cluster analysis clearly characterized all 12 tea varieties into three clusters based on their types namely China, Assam and Cambod. Pair-wise genetic similarity index between parent and hybrid clones generated showed a highest mean of 0.59 between 'TRI-2025' and 'BSS-1' and a lowest of 0.34 between 'UPASI-10' and 'BSS-1'. This study revealed that all the varieties analysed fall the present taxonomic framework of Camellia species and that the hybrid is of Cambod type. RAPD markers can thus be successfully applied in this taxon for the study of relationships and to confirm hybrid origin. The study offers a sound platform for future tea breeding programmes in tea as well as evolution of hybrids in the commercially important tea varieties.
Nucleation and Atmospheric Aerosols, 2019
Tea is a cross-pollinated and self-incompatible plant. Consequently, it has a very high genetic diversity. This research was aimed to estimate the variability of morphological and molecular characteristics of five accessions which exploited to develop high quality and quantity yield at PT. Pagilaran and compare the efficiency and accuracy of the use of morphological and molecular markers in tea characterization. The accessions consist of three C. assamica tea accessions (TRI 2025, Cinyiruan 143, and Kiara 8), and two C. sinensis tea accessions (Tambi and Tambi Jingga). Morphological observation includes leaf length, leaf width, stalk length, total pekoe, fresh leaf weight, stalk fresh weight, fresh pekoe weight, dry leaf weight, stalk dry weight, and pekoe dry weight, while the molecular observation was performed using SSR markers. Morphological data were analysed using 5 % ANOVA with Tukey-Kramer test and PCA Biplot using SAS 9.4 and R software, whereas the molecular binary data were analysed using the GenAlEx 6 software to estimate variance components, the percentage of polymorphism, and the total number of alleles and specific loci. The dendrogram was created using the Cluster procedure in SAS 9.4. The results showed that molecular characterization provides SSRs markers are more effective for putative genetic markers characterization, and every accession has its own morphological and SSR putative markers.
morphological characters in tea diversity
Morphological diversity of three Camellia (Theaceae) taxa conserved in an ex situ gene bank was studied and the importance of different descriptors in categorising accessions into distinct groups was also determined. Twelve accessions were characterised using 15 morphological descriptors of IPGRI guidelines. The results of principal component analysis (PCA) on morphological characters showed that the first two principal components accounted for 44.77 % of the total variance. In the evaluated quantitative characters, all three taxa had a coefficient of variation (CV) greater than 24.85%, and within the taxon the CV was greater than 9.59%. The qualitative characters showed a wide range of variations and yielded significant differences (p<0.05). Phenotypic data had high contributing component loadings from characters such as leaf area, weight of harvested shoots, stem colour, leaf pubescence and young shoot colour. Cluster analysis delineated the accessions into three groups. The implications of our results hold promise for assessing genetic diversity in germplasm collections, which is a prerequisite for their utilisation, effective management and crop improvement.
Plant Science, 2003
Amplified Fragment Length DNA Polymorphism (AFLP) analysis of 49 tea cultivars from south India produced a total number of 1555 unambiguous polymorphic amplified DNA fragments. The dendrogram derived by unweighted pair group method with arithmetic mean algorithm (UPGMA) analysis and the PCO plot drawn using principal component analysis revealed that all these tea cultivars could be clearly distinguished into three distinct groups viz., Assam, China and Cambod as well as an intermediate. Among the populations characterized, the Chinary type showed a maximum diversity index of 0.612 and the minimum of 0.285 was observed within the Assam type. Genetic distance was maximum (0.946), between Assam and Cambod and minimum (0.852) between Assam and China. More than 90% similarity as observed between the cultivars UPASI-22 and UPASI-23. Affinity of each cultivar towards the populations was determined using the similarity index. Analysis and comparison of AFLP fragments revealed distinct segregation of all the cultivars into their respective groups, except UPASI-18 and UPASI-24. Studies on diversity assessment of south Indian tea cultivars using AFLP fingerprinting revealed that the present day commonly grown south Indian tea germplasm has narrow genetic diversity (B/37.76) among the cultivars necessitating a sustained effort to preserve tea germplasm resources and the development of superior varietal material through wide genetic crosses.