Structure and Expression of a Novel Member, FGF-16, of the Fibroblast Growth Factor Family (original) (raw)

1998, Biochemical and Biophysical Research Communications

FGF family now consists of fifteen members (10)(11) 18). We have isolated cDNA encoding a novel member These FGFs also appear to play important roles as pep-(207 amino acids) of the FGF family from the rat heart tide growth factors in both developing and adult tisby homology-based polymerase chain reaction. As this sues. FGF-3 (int-2), FGF-4 (hst/kFGF), FGF-5 and protein is the 16th documented member of the FGF FGF-6 were identified as oncogene products (5-8). FGFfamily, we tentatively term it FGF-16. Among FGF fam-7 (KGF) was isolated as a mitogen for cultured keraily members, FGF-16 is most similar (73% amino acid tinocytes (9). FGF-8 and FGF-9 were isolated as an identity) to FGF-9. We have also determined the strucandrogen-induced growth factor and a glia-activating ture of human FGF-16 with high amino acid sequence factor from mouse mammary carcinoma cells and huidentity (98.6%) to rat FGF-16. Although the predicted man glioma cells, respectively (3, 4). FGF-10 was iden-FGF-16 amino acid sequence lacks a typical signal setified from rat embryos by homology-based polymerase quence, recombinant rat FGF-16 was efficiently sechain reaction (PCR) (10). These FGFs are predomicreted by Sf9 insect cells infected with recombinant nantly expressed during embryonic development and baculovirus containing the cDNA. FGF-16 mRNA was in restricted adult tissues. Furthermore, four novel predominantly expressed in the rat heart among the members, FGF homologous factors (FHFs), FHF-1 to adult major tissues examined. The expression profile of FGF-16 mRNA was quite different from those of FHF-4, were identified from the human retina by a other members of the FGF family. In rat embryos, FGF-combination of random cDNA sequencing, data base 16 mRNA was predominantly expressed in the brown searches and homology-based PCR (11). FHFs are preadipose tissue. However, the expression decreased dominantly expressed in the developing and adult nergreatly after birth. These results indicate that FGF-16 vous system. Coulier et al. proposed that FHF-1, FHFin embryos might play a role in development of the 2, FHF-3, and FHF-4 should be designated FGF-12, brown adipose tissue. ᭧