Isolation and Nucleotide Sequence of the PSA1 Gene from the Yeast Kluyveromyces Lactis (original) (raw)

Abstract

Isolation of Kluyveromyces lactis PSA1 gene was attempted using three different approaches, the successful one being complementation. A genomic clone of about 2.8 kb, containing the putative KlPSA1 gene, complemented the hypoosmotic instability of Saccharomyces cerevisiae srb1 mutant. Sequencing of the isolated genomic fragment revealed that it contains an ORF of 1083 bp, with 84% homology to ScPSA1 gene coding for a predicted polypeptide, Kl Psa1p, of 361 amino acids. Kl Psa1p showed high homology to yeasts’ GDP-mannose pyrophosphorylases. The nucleotide sequence of KlPSA1 has been deposited in the EMBL data bank under accession No. AJ551274.

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