Bifidobacterium breve and Streptococcus thermophilus secretion products enhance T helper 1 immune response and intestinal barrier in mice (original) (raw)
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Advances in Animal and Veterinary Sciences, 2016
L actobacillus acidophilus (LBA) selected as a probiotic bacteria because criteria that: suitable and are originate from human and other mammals; can resist bile, acid, enzyme and oxygen; with high affinity to intestinal mucosa adherence ; can easily colonize potential in the gastrointestinal tract; and can produce antimicrobial substances (Lee , 2009). In addition to the immunity stimulation, including activation of phagocytosis and production of antibodies or cytokines (Galdeano and Perdigon, 2006), different strains of LBA in experiments exert a beneficial effects on the gastrointestinal homeostasis (Alqayim and Jabbar, 2015) and in healing inflammatory bowel disease (Javed et al., 2016; Nova et al., 2016). Ulcerative colitis is one of two types of inflammatory bowel diseases that cause inflammation and ulcers in the colon resulting in disruption of normal colonic function, such as maintaining of fluid and electrolytes leads to defect in absorption and motility. The specific clinical signs of ulcerative colitis is diarrhea mixed with blood, even some times it become asymptomatic. Experimentally ulcerative colitis can be successfully induced by different models among them, intrarectal administration of diluted acetic acid (Low et al., 2013). Immunologically UC associated with abnormally responses, including increased production of pro-inflammatory cytokines particularly IL-6 and IL-8 and decreased others research Article Abstract | Inflammatory bowel diseases of two forms, Ulcerative colitis and Cron's disaes cause inflammation and ulcers in the colon resulting in disruption of normal colonic function and altered physiological aspects. The present study aimed to investigate the beneficial effects of Lactobacillus acidophilus (LBA) as a probiotics in reducing the severity of this disease. Forty eight male rats were distributed into four groups, each of 12. 1 st consider as control (C), 2 nd and 4 th (T1&T3) have colitis, 3 rd and 4 th (T2 &T3) administered Lactobacillus acidophilus in a dosage of (5 × 10 8 CFU). Colitis induced experimentally by intrarectal infusion of acetic acid. Food intake, body weight gain and food conversion ratio (FCR) recorded for 5 th and 25 the days of colitis Blood samples were collected from experimental animals at the days 1, 5 & 25 of experiment. Food intake and bodyweight gain were reduced in treated animals but FCR was improved by the administration of lactobacillus acidophilus improved FCR in spite of reduction of food intake and bodyweight gain. Total protein and globulins were increased by L. Acidophilus in normal and acetic acid colitis rats. Total WBCs lymphocytes, and platelets showed a gradual increase by L. acidophilus while neutrophils increased in acetic acid induced colitis. Immunologically, Leptin increased sharply during the 1 st in colitis followed by then followed by gradually decrease gradual decrease while in increased. Il-6 increased sharply in colitis and lactobasillus during 1 st day Administration of L. Acidophilus results in beneficial effects on the alteration of some physiological measurements of acetic acid induced colitis and make balance between proinflammatory and anti-inflammatory response.
Interaction of lactic acid bacteria with the gut immune system
European Journal of Clinical Nutrition, 2002
Health claims of lactic acid bacteria (LAB) used in functional foods and pharmaceutical preparations are based on the capacity of these microorganisms to stimulate the host immune system. In this study, the antigenic effect of LAB (Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus) on the gut immune system of BALB=c mice was evaluated. A dose-dependent increase of the Bcl2 protein was observed with all LAB assayed. Furthermore, the analysis of cytokine-producing cells in the lamina propria of gut showed that TNFa and INFg values, determined in macrophages cultured from Peyer patches, were enhanced for all the LAB assayed. An important increase of interleukins IL-10 and IL-4 was observed mainly in mice fed with Lactobacillus delbrueckii ssp. bulgaricus or Lactobacillus casei, while a significant induction of IL-2 and IL-12 was only observed with L. acidophilus (P < 0.01). These effects were dose dependent. The role of produced cytokines in the balance Th1=Th2 was determined by a systemic antibody response against parenterally injected ovoalbumin. L. casei, L. delbrueckii ssp. bulgaricus and L. acidophilus enhanced the IgG1 response favouring Th2 balance, while L. acidophilus also increased the IgG2a response inducing Th1 balance. S. thermophilus did not influence the balance Th1=Th2. Our studies showed that lactic acid bacteria induce distinct mucosal cytokine profiles showing different adjuvant capacity among them. Thus, selection of probiotic strain with immunological properties must be well defined to influence cytokine expression that favour the claimed immune response.
Applied and Environmental Microbiology, 2013
The present study was carried out to test the colonic mucosal response of rats to oral supplementation with Lactobacillus fermentum BGHI14 and to correlate the tissue reaction to trinitrobenzenesulfonate (TNBS)-induced colitis with mucosal barrier alterations caused by bacterial ingestion. An immune cell-mediated reaction of healthy colonic tissue was noticed after bacterial feeding. After prolonged bacterial treatment, the observed reaction had retreated to normality, but the mRNA levels of proinflammatory cytokines interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-␣) remained elevated. These data point to the chronic low-grade inflammation that could be caused by long-term probiotic consumption. Although no detrimental effects of bacterial pretreatment were noticed in colitic rats, at least in the acute state of disease, the results obtained in our study point to the necessity of reassessment of existing data on the safety of probiotic preparations. Additionally, probiotic effects in experimental colitis models might depend on time coordination of disease induction with treatment duration.
Journal of Food Science and Engineering, 2012
The effect of two fermented milks (FM 1 , FM 2) with lactic acid bacteria and Bifidobacteria on the intestine mucosa was studied. BALB/c mice were divided in groups of non-sensitized or sensitized by oral route to cow's milk or colonized by FM 1 [10 7 cfu/mL of Bifidobacterium longum (BfI) and 10 8 cfu/mL of Streptococcus thermophillus (StI)] and sensitized by oral route to cow's milk or colonized by FM 2 [10 7 cfu/mL of BfI and 10 7 cfu/mL of Lactobacillus plantarum (LbO)] and sensitized to cow's milk. Blood was sampled and the amount of anti-β-Lactoglobulin (β-Lg) IgG was measured. Mice were sacrificed, fragments of their intestines were isolated to inspect the structural changes of intestinal mucosa. A significant anti-β-Lg response was elicited by oral sensitization in positive control compared to other groups. Inspection of villi structural changes reveal signs of inflammation in challenged group compared with FM 1 and FM 2 groups, which conserved long villi characteristic of negative controls. The colonization of intestines by BfI-StI and BfI-LbO, and the evaluation of the residual antigenicity of β-Lg in mice sensitized to bovine milk by oral route followed by histological studies, revealed that FM 1 and FM 2 play protective role and reduce the histological lesions typical of bovine milk allergy.
Correlation between in vitro and in vivo immunomodulatory properties of lactic acid bacteria
World Journal of Gastroenterology, 2007
To investigate the correlation between in vitro and in vivo immunomodulation potential of the probiotic strain and its ability to prevent experimental colitis in mice. In vitro immunomodulation was assessed by measuring interleukin (IL)-12p70, IL-10, tumor necrosis factor alpha (TNFalpha) and interferon gamma (IFNgamma) release by human peripheral blood mononuclear cells (PBMCs) after 24 h stimulation with 13 live bacterial strains. A murine model of acute TNBS-colitis was next used to evaluate the prophylactic protective capacity of the same set of strains. A strain-specific in vivo protection was observed. The strains displaying an in vitro potential to induce higher levels of the anti-inflammatory cytokine IL-10 and lower levels of the inflammatory cytokine IL-12, offered the best protection in the in vivo colitis model. In contrast, strains leading to a low IL-10/IL-12 cytokine ratio could not significantly attenuate colitis symptoms. These results show that we could predict the in vivo protective capacity of the studied lactic acid bacteria (LAB) based on the cytokine profile we established in vitro. The PBMC-based assay we used may thus serve as a useful primary indicator to narrow down the number of candidate strains to be tested in murine models for their anti-inflammatory potential.
Selecting Lactic Acid Bacteria for Their Safety and Functionality by Use of a Mouse Colitis Model
Applied and Environmental Microbiology, 2006
Studies showed that specific probiotics might provide therapeutic benefits in inflammatory bowel disease. However, a rigorous screening of new probiotics is needed to study possible adverse interactions with the host, particularly when intended for administration to individuals with certain health risks. In this context, the objective of this study was to investigate the role of three lactobacilli (LAB) on intestinal inflammation and bacterial translocation using variations of the mouse model of 2,4,6-trinitrobenzene sulfonic acid (TNBS)induced acute colitis. We first compared the in vitro ability of LAB to survive gastrointestinal tract (GIT) conditions and their ability to persist in the GIT of mice following daily oral administration. As a control, we included a nonprobiotic Lactobacillus paracasei strain, previously isolated from an endocarditis patient. Feeding high doses of LAB strains to healthy and to TNBS-treated mice did not induce any detrimental effect or abnormal translocation of the bacteria. Oral administration of Lactobacillus salivarius Ls-33 had a significant preventive effect on colitis in mice, while Lactobacillus plantarum Lp-115 and Lactobacillus acidophilus NCFM did not. None of the three selected LAB strains translocated to extraintestinal organs of TNBS-treated mice. In contrast, L. paracasei exacerbated colitis under severe inflammatory conditions and translocated to extraintestinal organs. This study showed that evaluations of the safety and functionality of new probiotics are recommended. We conclude that not all lactobacilli have similar effects on intestinal inflammation and that selected probiotics such as L. salivarius Ls-33 may be considered in the prevention or treatment of intestinal inflammation.
The Immunostimulatory Effect of Lactic Acid Bacteria in a Rat Model
Iranian journal of immunology : IJI, 2016
BACKGROUND Probiotics are "live", beneficial microbes that provide important health benefits in their hosts. There is significant interest in the modulation and regulation of the immune function by probiotics. OBJECTIVE To investigate the immunomodulatory effects of a probiotic mixture, including Lactobacillus and Bifidobacterium species, by detecting serum cytokine and immunoglobulin levels. METHODS The rats were randomly divided into 4 groups. The first group was "Control group" and other 3 groups were probiotic application groups who received different doses of probiotics. The probiotic mixture included 12 probiotic bacteria, mostly Lactobacillus and Bifidobacterium strains. Probiotic mixture was administered to rats for 12 consecutive days. TNF-α, TGF-β, IL-1-β, IL-6, and IL-10 levels as well as serum IgG and IgA concentrations were detected in the sera after 12 days. RESULTS Probiotics led to a decrease in the levels of TNF-α, IL-6 and TGF-β; however, they l...
Clinical and Developmental Immunology, 2011
Studies showed that specific probiotics provide therapeutic benefits in inflammatory bowel disease.In vitroevidence suggested thatLactobacillus paracaseialso called ST11 (CNCM I-2116) is a potent strain with immune modulation properties. However, little is known about its capacity to alleviate inflammatory symptomsin vivoIn this context, the main objective of this study was to investigate the role of ST11 on intestinal inflammation using the adoptive transfer mouse model of experimental colitis. Rag2-/-recipient mice were fed with ST11 (109CFU/day)a month prior toinduce colitis by adoptive transfer of naive T cells. One month later, in clear contrast to nonfed mice, weight loss was significantly reduced by 50% in ST11-fed mice. Further analysis of colon specimens revealed a significant reduction neutrophil infiltration and mucosal expression of IL1β, IL-6, and IL12 proinflammatory cytokines, whereas no consistent differences in expression of antibacterial peptides or tight junction ...