Highperformance liquid chromatographic analysis of trimethoprim and sulfamethoxazole in microliter volumes of chinchilla ear effusion and serum (original) (raw)

Journal of Chromatography A

Abstract

Simple, sensitive and reliable high-performance liquid chromatographic methods are reported for the determination of pindolol enantiomers in human serum and urine. The methods involved a solid-phase extraction of serum and a direct injection of urine samples. The separation of R(+)- and S(-)-pindolol was accomplished on a reversed-phase cellulose-based chiral column with a mobile phase of 40:60 (v/v) acetonitrile-0.3 M aqueous sodium perchlorate at a flow-rate of 0.5 ml/min. The detection was achieved by monitoring the fluorescence emission of pindolol enantiomers at 310 nm with excitation at 270 nm. The limits of detection were 1.2 ng/ml of R(+)- and 4.3 ng/ml of S(-)-pindolol in serum, and 21 ng/ml of R(+)- and 76 ng/ml of S(-)-pindolol in urine. The external standard method was used for quantitation. The methods have been applied to the analysis of human serum and urine samples in a pharmacokinetic study.

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