The protective role of pomegranate juice against carbon tetrachloride-induced oxidative stress in rats (original) (raw)
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Antioxidant and antiatherogenic effects of pomegranate
American Journal of Health-System Pharmacy, 2011
Pomegranate molasses (PM) is a large compound of the eastern diets, yet no research has been performed on this product. In this study, we measured the total polyphenols content of PM, compared to fresh pomegranate juice, obtaining 252.28 and 79.49 mg of Gallic Acid equivalent/L respectively. The antioxidant effect of PM and juice was then measured in vitro using electrolysis as a free radical generating system. At the concentrations of 100 to 600 l PM has strong antioxidant properties (4 times more active than juice). Moreover, molasses or juice were added to the drinking water of mice (4 ml/l) during 11 weeks leading to a significant decrease of weight curve compared to control animals; also triglycerides and lipid peroxidation were decreased in the heart, lungs, and the liver, while superoxide dismutase activity increased. In conclusion, Pomegranate molasses possesses a powerful antioxidant activity and a weight loss effect in mice.
Biosciences Biotechnology Research Asia, 2014
Pomegranate (Punica granatum L.) is rich in polyphenolic antioxidant, which includes tannins and anthocynins and flavonoids. Researchers reported that pomegranate exhibits antivirus, anti-proliferative, anti-cancer, antidiarrheal and anti-diabetic activities. The aim of this study was to investigate the antioxidant properties of pomegranate on kidney, liver and testis tissues of male rats. Eighteen rats were divided into three groups; Group 1, received distilled water; animals in the Groups 2 and 3 received hydro-alcoholic extract of pomegranate in doses of 250 and 500 mg kg-1 respectively. The extract was administrated for 8 weeks by gavage, then oxidative stress and antioxidant enzymes were studied in kidney, liver and testis tissues of all groups.-The hydro-alcoholic extract of pomegranate significantly decreased (p<0.05) malondialdehyde (MDA) level, but increased (p<0.05) the superoxidedismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) activity of kidney, liver and testis when compared to control group. In conclusion, hydro-alcoholic extract of pomegranate possesses a powerful antioxidant activity and can increase levels of antioxidant enzymes in some tissues.
Lipid peroxidation is a crucial step in the pathogenesis of free radical-related disease states, including glomerulonephritis, rheumatoid arthritis, gastrointestinal and cardiovascular diseases. Antioxidants play a significant role in protecting living organism from the toxic effect of various chemicals by preventing free radical formation. Pomegranate is a good source of polyphenols and other antioxidants. The purpose of the present study is to evaluate the effect of pomegranates juice and peel administration on antioxidant enzymes activities and lipid profile in carbon tetrachloride-induced hyperlipidemic rats. Hyperlipidemic rats were treated with pomegranates juice and peel for 8 weeks. Results showed that all hyperlipidemic rats administrated juice and peel had significant decrease in TBARS concentration and increase in GSH, GPx, GST, CAT and SOD in plasma compared to CCl4 group. Lipid profile parameters (except HDL-C) decreased significantly with juice and peel administration compared to CCl4 group. In conclusion, consumption of pomegranate juice and peel showed significant improvement of lipid parameters and antioxidant enzyme activities in hyperlipidemic rates suggesting therapeutic potential of using pomegranate as a medicinal plant for managing hyperlipidemia and modifying the risk of CVD.
Antioxidative potential of pomegranate peel extract: In vitro and in vivo studies
Scripta medica, 2023
Background/Aim: Due to the numerous beneficial effects of pomegranate that can be explained through its antioxidative effects, the aim of this study was to determine the antioxidant potential of pomegranate peel extract (PoPEx) prepared from pomegranate that was harvested in the southeast region of Herzegovina (Bosnia and Herzegovina), through in vitro and in vivo studies. Methods: In PoPEx total phenols, flavonoids, flavonols, flavan-3-ols and anthocyanins content was determined, as well as several antioxidative assays, including 2,2 diphenyl-1-picrylhydrazyl assay (DPPH), 2,2'-azino bis(3-ethylbenzothiazoline-6-sulphonic acid) assay (ABTS), iron (III)-2,4,6-tripyridyl-S-triazine complex assay (FRAP), reduction of copper(II) ions (CUPRAC) assay, Briggs-Rauscher oscillatory reactions, neutralisation of OH radicals and lipid peroxidation assay. In vivo studies were performed by administrating 100 mg/ kg of body weight of PoPEx to the rats by gavage for 7 days, after which the rats were euthanised and prooxidative parameters (thiobabrituric acid reactive substances-TBARS as an index of lipid peroxidation, nitrites-NO 2 , hydrogen peroxide-H 2 O 2 and superoxide anion radical O 2-) were determined in plasma, as well as antioxidative parameters (superoxide dismutase-SOD, reduced glutathione-GSH and catalase-CAT) in erythrocyte lysates. Results: High content of phenolic compounds was found in PoPEx, which resulted in high antioxidative potential in all in vitro tests performed. In vivo study showed that PoPEx administration caused a significant decrease in TBARS, NO 2-, as well as an increase in reduced glutathione (p < 0.05) in comparison to the control group, while H 2 O 2 and O 2 * showed a lowering trend and SOD and CAT showed an increasing trend in PoPEx group, but without statistical significance. Conclusion: PoPEx demonstrated high antioxidative capacity measured in vitro and in vivo and can be potentially used as a supplement treatment in the prevention of various inflammatory conditions.
In vitro and In vivo studies on antioxidant potential of methanolic extract of Pomegranate fruits
The present study was aimed at investigating the antioxidant activities of the methanolic extract of pomegranate (Punica granatum) fruit. The antioxidant activities of extract have been evaluated by using in vitro assays and in vivo model. In case of in vitro studies, the antioxidant activity of methanolic extract of pomegranate fruit was 198.31 mg/100g; 192.25 mg/100g; 181.13 mg/100g and 143.24 mg/100g by DPPH, nitric oxide, superoxide radical scavenging assays and ferric reducing antioxidant power assay, respectively. In case of in vivo studies, the levels of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and lipid peroxidation (LPO) were restored towards the normal value in fruit supplemented alloxan induced diabetic rats. The free radical scavenging and antioxidant activities may be attributed to the presence of total poly phenols (148.61 mg/100g), total flavonoids (209.83 mg/100g) and ascorbic acid content (24.21 mg/100g) present in pomegranate fruit. The results obtained in the present study indicate that pomegranate fruit is a potential source of natural antioxidant.
The pomegranate seed oil (PSO) presents a high content of the punicic acid (PA), which is a conjugated isomer of α-linolenic acid (CLNA). Conjugated fatty acids have increasingly attracted scientific interest because of their several potential health benefits, including antioxidant activity. The aim of this study was to evaluate the supplementation effect of PSO on oxidative stress markers in rats subjected to liver injury by carbon tetrachloride (CCl4 ). Animals were supplemented with PSO at concentrations of 2% and 4%, for 21 days, and then subjected to liver injury induced by CCl4 . The PSO supplementation reduced serum aminotransferase enzymes and the percentage of liver damage induced by CCl4 . Conversely, PSO supplementation increased the lipid hydroperoxides levels in the liver. Overall, these results suggest that supplementation with PSO is not capable of attenuating markers of oxidative stress caused by CCl4
International Journal of Research, 2016
Pomegranate peel extracts (PPE) have been shown to possess significant antioxidant activity. This study aimed to evaluate the protective effect of PPE on carbon tetrachloride (CCl4)-induced toxicity in mice. Forty adult mice were divided into four groups of 10 mice each. The first group was kept as a control. The second group was administered orally by PPE (400 mg/kg) for 15 days. The third group was intraperitoneally injected by a mixture of (1ml/kg) of CCl4 and sterile olive oil [1:1(v/v)] two times a week for 15 days. The fourth Group was injected (IP) by a mixture of (1ml/kg) of CCl4 and sterile olive oil [1:1(v/v)] two times a week concurrently with a daily oral dose of PPE (400 mg/kg) for 15 days. The biochemical studies showed that CCl4intoxication induced a significant increase in serum of aspartate amino transferase (AST) and alanine amino transferase (ALT) levels and treatment with PPE showed decreased in these elevated enzymes. The histological examination of liver sectio...
Mixed Pro- and Anti-Oxidative Effects of Pomegranate Polyphenols in Cultured Cells
International Journal of Molecular Sciences, 2014
In recent years, the number of scientific papers concerning pomegranate (Punica granatum L.) and its health properties has increased greatly, and there is great potential for the use of bioactive-rich pomegranate extracts as ingredients in functional foods and nutraceuticals. To translate this potential into effective strategies it is essential to further elucidate the mechanisms of the reported bioactivity. In this study HepG2 cells were supplemented with a pomegranate fruit extract or with the corresponding amount of pure punicalagin, and then subjected to an exogenous oxidative stress. Overall, upon the oxidative stress the gene expression and activity of the main antioxidant enzymes appeared reduced in supplemented cells, which were more prone to the detrimental effects than unsupplemented ones. No differences were detected between cells supplemented with the pomegranate juice or the pure punicalagin. Although further studies are needed due to the gaps existing between in vitro and in vivo studies, our results suggest caution in the administration of high concentrations of nutraceutical molecules, particularly when they are administered in concentrated form.