Screening of δ-aminolevulinic acid dehydratase from Pseudomonas strains as biosensor for lead and some other metals contamination (original) (raw)

Bio-Monitoring of Heavy Metal Resistance in Pseudomonas and Pseudomonas Related Genus

2013

The aim of present study was to determine the level of heavy metal resistance patterns and to determine if there is a relationship between heavy metal resistance and plasmid presence. From 28 identified strains, 39.3% corresponded to Stenotrophomonas maltophilia, 17.9% to Chryseomonas luteola, 14.3% to Pseudomonas fluorescens, 10.7% to Pseudomonas putida, 7.1% to Sphingomonas paucimobilis, 7.1% to Methylobacterium mesophilicum and 3.6% Pseudomonas stutzeri. The resistance of these Gramnegative bacteria to 8 heavy metals, was investigated by agar dilution method. Most isolates showed tolerance to different concentrations of heavy metals, and minimal inhibition concentrations ranged from 0,005 mmol-20 mmol. All strains displayed high resistance to zinc and lead (100% and 96,4% respectively) and high susceptibility to silver, cobalt and mercury (92.9%, 92.9% and 96.4% respectively). M. mesophilicum strains were determined as the most resistant strains to studied heavy metals. Isolated ...

Trends in Heavy Metals Tolerance and Uptake by Pseudomonas aeruginosa

Clarivate , 2019

Pseudomonas aeruginosa is considered as the most potent bacterial strain for solving heavy metals pollution problems. Pollution is the most of problems in our world which causing a lot of risks to human, animal, plant and ecosystem. Heavy metals pollution is an ever-increasing problem in developing nations. Release of heavy metals into the environment has increased in the recent years at an alarming rate. To remove heavy metals from environment, there are different methods such as physical, chemical and biological. The biological method includes microorganisms and plant which recorded high heavy metals removal, safe and low-cost method. Microorganisms remove heavy metals from environment by different mechanisms according to their types. Thus, microbes are used as potential candidates of bioremediation that can adapt quickly to the changing noxious environment and be utilized for toxic metal remediation. In bacterial uptake and tolerance to heavy metals, Pseudomonas aeruginosa recorded potential role in bioremediation of different heavy metals with high removal percentage comparison with other bacterial strains. Chapter discusses the roles and trends of Pseudomonas aeruginosa in heavy metals tolerance and uptake as potential bacterial strain.

Heavy Metal Tolerance Pattern of Pseudomonas putida Isolated from Heavy Metal Contaminated Soil of Zawar, Udaipur (India)

Heavy metal pollution has become one of the most serious environmental problems. The pollution of environment with toxic heavy metals is spreading throughout the world along with industrial progress. Developing bioremediation processes for soils and effluents contaminated by heavy metals is one of the most prime concern. For the same, extensive screening for the bacteria having tolerance to heavy metals such as zinc, cadmium, cobalt, nickel, copper and lead was attempted. During this isolate HMR37 was recovered from heavy metal polluted tailing site of Zawar, Udaipur on heavy metal amended nutrient agar medium having multiple metal tolerance. Based on the morphological, cultural and biochemical characterization, the isolate HMR37 presumptively identified as Pseudomonas putida. Tolerance of HMR37 strain based on their maximum tolerable concentrations (MTCs), was in the order of Zn>Cd=Co>Ni>Cu>Pb. Maximum growth of this isolate at threshold concentration of respective metal was found at pH 7.0 and temperature 37°C. This isolate can further be successfully exploited biotechnologically for the bioremediation of heavy metal contaminated environment.