Structural relationships between the RNA of mammary tumor virus and those of other RNA tumor viruses (original) (raw)
Related papers
Physical properties of Rous Sarcoma Virus RNA
Proceedings of the National Academy of Sciences of the United States of America, 1968
The RNA's of several RNA tumor viruses have recently been isolated.1 Two major single-stranded RNA components were obtained, a fast-sedimenting RNA component with a sedimentation coefficient (820,w) of approximately 70S and a slowly sedimenting component of approximately 4S. Since the reported st9 as well as the corresponding molecular weights of the tumor virus RNA's are much larger than those of most other known viral RNA's, a determination of the structure and molecular weight of tumor virus RNA's is of great interest. Knowledge of the structure of tumor virus RNA's may also help to elucidate their replication, which is interrupted by inhibitors of DNA-dependent RNA synthesis such as actinomycin D;2 in addition, no virus-specific double-stranded RNA's have been isolated from infected or transformed cells.
Isolation of the nucleic acid of mouse mammary tumor virus (MTV)
Proceedings of the National Academy of Sciences, 1966
The mouse mammary tumor virus (MTV) was one of the first tumorigenic viruses found in mammals.1' 2 Nevertheless, little progress has been made on the elucidation of the molecular structure of the virus.3 The absence of a rapid bioassay has hindered progress in virus purification, and the difficulty in growing the virus in tissue culture has made use of radioisotopes impractical.4 MTV is normally transmitted thrQugh the mother's milk. This establishes a lifelong infection, and virus can be recovered from tissue or milk at any time. The ultimate development of a mammary tumor in an infected female is dependent not only upon the presence of the virus but also upon genetic and hormonal factors.
Properties and location of poly(A) in Rous sarcoma virus RNA
Journal of Virology
The poly(A) sequence of 30 to 40S Rous sarcoma virus RNA, prepared by digestion of the RNA with RNase T, showed a rather homogenous electrophoretic distribution in formamide-polyacrylamide gels. Its size was estimated to be about 200 AMP residues. The poly(A) appears to be located at or near the 3' end of the 30 to 40S RNA because: (i) it contained one adenosine per 180 AMP residues, and because (ii) incubation of 30 to 40S RNA with bacterial RNase H in the presence of poly(dT) removed its poly(A) without significantly affecting its hydrodynamic or electrophoretic properties in denaturing solvents. The viral 60 to 70S RNA complex was found to consist of 30 to 40S subunits both with (65%) and without (approximately 30%) poly(A). The heteropolymeric sequences of these two species of 30 to 40S subunits have the same RNase T,-resistant oligonucleotide composition. Some, perhaps all, RNase T,-resistant oligonucleotides of 30 to 40S Rous sarcoma virus RNA appear to have a unique location relative to the poly(A) sequence, because the complexity of poly(A)-tagged fragments of 30 to 40S RNA decreased with decreasing size of the fragment. Two RNase T,-resistant oligonucleotides which distinguish sarcoma virus Prague B RNA from that of a transformation-defective deletion mutant of the same virus appear to be associated with an 11S poly(A)-tagged fragment of Prague B RNA. Thus RNA sequences concerned with cell transformation seem to be located within 5 to 10% of the 3' terminus of Prague B RNA.
Molecular weight of RNA subunits of Rous sarcoma virus determined by electron microscopy
Journal of Virology, 1975
Secondary cultures of chicken embryo fibroblasts were infected with the Schmidt Ruppin strain of Rous sarcoma virus (RSV). Five days after infection, the medium was replaced at 2-h intervals with phosphate-free Eagle medium containing 50 muCi of [32P]orthophosphate per ml. Virus was collected by centrifugation, and the RNA was extracted and denatured with dimethyl sulfoxide, and the 33S subunit RNA was isolated by sucrose gradient centrifugation. The molecular weight of the RSV subunit RNA was determined by length measurement in the electron microscope, by using bacteriophage MS2 RNA as a length marker. Molecules of between 2.5 and 3.3 mum in length made up over 50% of the subunit RNA preparation. In this paper, we define RSV RNA subunits as that RNA released from the 70S RNA complex by dimethyl sulfoxide treatment, which sediments as a peak at 33S. Assuming the molecular weight of MS2 RNA to be 1.2 times 10-6, we calculate the molecular weight of RSV subunit RNA to be 3.12 times 10...
Comparative analysis of RNA tumor virus genomes
Haematology and blood transfusion, 1979
G 3 2~-~ RNA en in Pedersen and Haseltine, 1978 and diagrammed in ) involve specific cleavage of a small amount of non-radioactive RNA after guanosine residues by ribonuclease Tl followed by 5'-32P-labelling of the Tl resistant oligonucleotides. The mixture of 5'-32P-labelled oligonucleotides is then fractionated by two-dimensional gel electrophoresis. By autoradiography of the gel these oligonucleotides will show a Pattern (termed a RNase Tl fingerprint) which is characteristic for each RNA sample. For further analysis the unique oligonucleotides can be eluted and their nucleotide sequence determined using recently developed methods for RNA sequencing that depend upon partial digestion of 5' end-labelled RNA with ribonucleases that cleave the RNA at specific nucleotides .
Characterization of the Low-Molecular-Weight RNAs Associated with the 70S RNA of Rous Sarcoma Virus
Journal of Virology, 1973
Two low-molecular-weight RNAs are associated with the 70 S RNA complex of Rous sarcoma virus: a previously described 4 S RNA and a newly identified 5 S RNA. The 4 S RNA constitutes 3 to 4% of the 70 S RNA complex or the equivalent of 12 to 20 molecules per 70 S RNA. It exhibits a number of structural properties characteristic of transfer RNA as revealed by two-dimensional electrophoresis of oligonucleotides obtained from a T1 ribonuclease digest of the 4 S RNA species. The 5 S RNA is approximately 120 nucleotides in length, constitutes 1% of the 70 S RNA complex or the equivalent of 3 to 4 molecules per molecules of 70 S RNA, and is identical in nucleotide composition and structure to 5 S RNA from uninfected chicken embryo fibroblasts. Melting studies indicate that the 5 S RNA is released from the 70 S RNA complex at the same temperature required to dissociate 70 S RNA into its constituent 35 S subunits. In contrast, greater than 80% of the 4 S RNA is released from 70 S RNA prior to...
Structure and Molecular Weight of the 60-70S RNA and the 30-40S RNA of the Rous Sarcoma Virus
Proceedings of the National Academy of Sciences, 1974
The structure and molecular weight of the 60-70S RNA complex and the 30-40S RNA species of Rous sarcoma virus were analyzed in an electron microscope after treatment of the RNAs with the bacteriophage T4 gene-32 protein to stretch out the RNA strands. Although all RNA preparations treated with gene-32 protein showed considerable heterogeneity in length, a significant fraction
Journal of virology, 1972
Cells producing Rous sarcoma virus contain virus-specific ribonucleic acid (RNA) which can be identified by hybridization to single-stranded deoxyribonucleic acid (DNA) synthesized with RNA-directed DNA polymerase. Hybridization was detected by either fractionation on hydroxyapatite or hydrolysis with single strand-specific nucleases. Similar results were obtained with both procedures. The hybrids formed between enzymatically synthesized DNA and viral RNA have a high order of thermal stability, with only minor evidence of mismatched nucleotide sequences. Virus-specific RNA is present in both nuclei and cytoplasm of infected cells. This RNA is remarkably heterogeneous in size, including molecules which are probably restricted to the nucleus and which sediment in their native state more rapidly than the viral genome. The nature of the RNA found in cytoplasmic fractions varies from preparation to preparation, but heterogeneous RNA (ca. 4-50S), smaller than the viral genome, is always p...