Hematology of juvenile pacu, piaractus mesopotamicus (Holmberg, 1887) fed graded levels of mannan oligosaccharides (MOS) (original) (raw)
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Hematology of juvenile pacu, Piaractus mesopotamicus (Holmberg, 1887) fed graded levels of mannan
Latin American Journal of Aquatic Research, 2014
Intensification of aquaculture production systems exposes fish to numerous stressors, which may negatively affect their health. This study determined the effects of increasing levels of dietary mannan oligosaccharides (ActiveMOS ® -Biorigin) on biochemical and hematological parameters of juvenile pacu, Piaractus mesopotamicus. Fish (44.04 ± 5.27 g) were randomly distributed into 24 tanks (500 L; 10 fishes per tank) and fed during 63 days with a commercial diet supplemented with 0.0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.5 and 2.0% MOS. Blood samples were collected at 42 and 63 trial. Red blood cell count (RBC) and total plasmatic protein were affected by dietary MOS levels (P < 0.05). Fish fed 1.0% dietary MOS presented higher neutrophils numbers when compared to fish fed control diet and fish fed 1.5% MOS for 42 days presented significant higher granulocytic cell numbers. During trial fish presented increased (P < 0.05) hematocrit, mean corpuscular volume, mean corpuscular hemoglobin and plasmatic glucose concentrations and decreased (P < 0.05) RBC, hemoglobin concentration, mean corpuscular hemoglobin concentration, white blood cell and differential leukocyte count. Dietary MOS levels did not present prebiotic effects for pacu and did not minimize stress effects on hematological and biochemical parameters for the species.
Journal of The World Aquaculture Society, 2008
Impaired immune system by environmental stressors can lead fishes to be more susceptible to diseases that limit the economic development of aquaculture systems. This study was set out to determine the effect of six levels of mannan oligosaccharides (MOS; ActiveMOS®; Biorigin, Lençois Paulista, São Paulo, Brazil) on the performance index and hematology of Nile tilapia, Oreochromis niloticus juveniles. Fish (13.62 g) were randomly distributed into 18 plastic aquaria (300 L; 20 fishes per aquarium) and fed during 45 d with a commercial diet supplemented with 0, 0.2, 0.4, 0.6, 0.8, and 1% dietary MOS, in a totally randomized design trial (n = 3); biometrical and hematological data were collected and analyzed. There were no significant differences in hematological parameters between fish fed control and MOS supplementation diets, and daily feed consumption (FC) decreased (P < 0.05) with increasing levels of dietary MOS. Dietary MOS did not increase leukocyte count and presented negative effects on FC of Nile tilapia. At 0.4% MOS supplementation, the individual weight gain was higher in absolute values but not different (P > 0.05) compared to control diet.
Effect of dietary mannan oligosaccharide (MOS; activeMOS ® ) on growth, survival, body composition and some hematological parameters in carp (Cyprinus carpio) juvenile were investigated for 45 days. Basal diet were supplemented with 0 (control), 1.0, 2.0 and 3.0 g kg -1 MOS in a totally randomized design trial in triplicate groups. The experiment carried out in 100 liters PVC tanks. 20 juvenile carp with initially average weight 1.3 ± 0.17 g were stocked in tanks and fed up a day. There were no significant differences in growth and feeding parameters between fish fed control and MOS supplementation diets (P>0.05). The highest and the lowest growth performance were observed in 1.0 g kg -1 MOS and control treatment, respectively. There were no significant differences in survival rate and body composition among experimental groups (P>0.05), but in group treated with 1.0 g kg -1 MOS showed higher protein carcass (P>0.05) than other group. An elevation of hematocrit, lymphocyte (P<0.05), WBC, RBC, Hb and eosinophil (P>0.05) were found in the fish fed diet 1.0 g kg -1 MOS. The result indicated that 1.0 g kg -1 MOS can improved growth performance, survival, final production and some blood parameters of carp juvenile and it is appropriate for supplementation in the diet of cultured juvenile carp.
Aquaculture International, 2007
The objective of these experimental trials was to determine the effect of a mannan oligosaccharide (MOS) derived from the outer cell wall of Saccharomyces cerevisiae strain 1026 on the growth performance and immune status of rainbow trout. Two experiments were conducted, one with eight net cages and the other with eight raceways. The net cage experiment (42 days) involved 14,400 fish with an initial average weight of 30 g. The raceway experiment (90 days) was conduced with 40,000 fish with an initial average weight of 101 g. Both experiments compared a commercial extruded diet with and without 2,000 ppm MOS supplementation. The calculated daily feed was supplied in six equal rations. Body weight, feed intake, and mortality were recorded and samples were taken for analysis of indicators of immune status. All data were subjected to ANOVA, with a net cage or raceway regarded as an experimental unit. Significantly improved performance and immune status were observed in the net cage trial-improved weight gain of 13.7% (P < 0.01), reduced feed conversion ratio (FCR) (P < 0.05), reduced mortality (P < 0.01), and improved indicators of immune status (P < 0.01) for fish fed the MOS supplement compared with controls. Similar significantly improved performance was observed for the MOS-treated groups in the raceway trial-9.97% improved weight gain (P < 0.01), lower FCR (P < 0.01), and reduced mortality compared with the control treatment. In the raceway trials, however, only the indicators of immune status lysozyme concentration, APCA, and CPCA were significantly improved by MOS treatment (P < 0.05). These experimental trials demonstrated the ability of MOS to improve the growth performance, survival, and immune status of rainbow trout produced in net cages or raceways.
Journal of Aquatic Animal Health, 2012
A feeding trial was performed to assess the potential beneficial effect of two levels of mannanoligosaccarides (MOS) on the growth performance, feed utilization, hematological parameters, and liver histopathology of gilthead seabream Sparus auratus (also known as gilthead bream). Mannanoligosaccarides were added at the rates of 2 and 4 g/kg to a fish-meal-based control diet, and each diet was given (twice daily [midmorning and midafternoon] to apparent satiation) to triplicate groups of gilthead seabream growers (mean weight = approximately 170 g) in sea cages. The trial lasted 12 weeks, and the average ambient water temperature ranged from 19.6°C to 24.7°C during the experimental period. At the end of the experiment, fish attained market size (350–450 g) and their health status was evaluated by blood analysis and liver histology. There were no differences in survival rates among fish fed experimental diets. However, there were significant improvements in both growth and feed utilization among fish fed diets supplemented with MOS. Hemoglobin (Hb) and hematocrit (Ht) levels and erythrocyte, leukocyte, and thrombocyte (Thr) counts were unaffected by any dietary MOS. The levels of Hb (g/dL; mean ± SD) and Ht (%; mean ± SD) were 11.0 ± 2.5 and 45.6 ± 6.7 for the control group, 11.1 ± 1.7 and 39.3 ± 8.0 for the 2-g/kg group, and 11.2 ± 1.9 and 40.2 ± 8.4 for the 4-g/kg group. The mean Thr count ranged from 47.6 to 53.8 × 103/mm3. Despite the apparently higher Thr counts for fish fed diets supplemented with MOS, these differences were not significant. Moreover, no histopathological differences were observed in liver tissue cross sections between control and treatment groups. These results suggest that supplementation of diets with MOS had no significant effects on general fish health.Received February 20, 2011; accepted September 12, 2011
Aquaculture Research, 2015
A feeding trial was conducted to study the effect of mannan oligosaccharide (MOS) supplementation on growth, body composition, haematology, biochemical parameters and histology of Asian seabass (Lates calcarifer) fingerlings (8.13 AE 0.06 g average weight). Mannan oligosaccharide was supplemented at five different concentrations viz., 0%, 0.5%, 1%, 1.5% and 2% levels in the diet (40% protein and 9% lipid) of L. calcarifer. The results of the 60 days feeding trial showed that there was a significant (P < 0.05) increase in the final body weight, weight gain (WG), WG%, average daily gain, survival, specific growth rate, daily growth coefficient, hepatosomatic index and viscerosomatic index in the fish fed diet containing 1% MOS. Whole body composition of post-fed animals showed non-significant differences (P > 0.05) among the various treatment groups. The analysis of haematological parameters showed that there was no significant (P > 0.05) differences among different treatments but for the haemoglobin content which was significantly (P < 0.05) higher in the fish fed 1% MOS. Biochemical parameters revealed that glucose, urea, cholesterol and triglyceride content showed significant (P < 0.05) difference between control and MOS-supplemented group. Histological observations of post-fed animals revealed that MOS supplementation resulted in increased absorptive surface area of the intestine and increased glycogen deposition in liver. The result of this experiment infers that MOS supplementation has got a beneficial effect in the diet of seabass and supplementation at 1% level is optimal for improving the growth.
The Israeli journal of aquaculture = Bamidgeh
A 90-days growth trial was conducted to determine the effects of prebiotic mannanoligosaccharides (MOS) on the histology and biochemical blood parameters of the gilthead seabream (Sparus aurata). Two experimental diets were formulated to contain MOS (Bio-Mos ®) levels ranging 0 and 2 g kg -1 . The fish averaging 172.11±13.19 g were reared in six octagonal net cages (763 m 3). Fish were fed twice daily to apparent satiation. Individual body weight, weight gain, survival rate and FCR were measured during the experiment. There were significant effects of diet on the differences between the groups in body weights and weight gain (P<0.05) but an insignificant effect on the survival rates and FCR (P>0.05). At the end of the experimental period, biochemical blood parameters were analyzed. Blood urea level was significantly affected in the MOS group (P<0.05). No remarkable alterations in histological examinations were found. This study highlighted the positive effects of prebiotic ...
Turkish Journal of Fisheries and Aquatic Sciences, 2018
The study investigated the effect of increasing dietary inclusion of M annan oligosaccharide (M OS) on growth and blood parameters of sea bream (Sparus aurata). Varying concentrations of M OS were added to commercial sea bream feed at 0‰ (control), 1, 2, 3 and 4‰. At the end of the 15 week trial, control group had the highest final weight, specific growth rate, feed conversion ratio and protein efficiency ratio (P<0.05), although these did not differ among the M OS containing groups (P>0.05). Survival rates were higher in the M OS treatment groups. Blood parameters, did not differ among treatment groups (P>0.05). The lowest alkaline phosphatase (ALP) value was measured in the control group whereas the highest was recorded in the 4‰ M OS group. The lowest level of triglycerides was measured in the 4‰ M OS group, while the highest level of triglycerides was measured in the control group. Cholesterol level was lowest in the 2‰ M OS group (P<0.05). The study showed that the inclusion of dietary MOS has no negative effects on growth and blood parameters in sea breams. However, dietary M OS inclusion led to higher survival rate in trial fish.
In this study, we examined effect of mannan oligosaccharides (MOS) on growth performance and immune response of gibel carp juveniles (Carassius auratus gibelio(. After acclimation, fish (6.87 ± 0.21 g) were allocated into 12 aquariums (20 fish per aquarium). Basal diet were supplemented with 0 (control), 1.5, 3 and 4.5 g kg -1 MOS in a totally randomized design trial in triplicate groups. At the end of the trial (8 weeks), growth factors (final weight, weight gain, specific growth rate (SGR), food conversion ratio (FCR), and condition factor) and immune responses (serum Ig levels, lysozyme activity and alternative complement activity (ACH50)) were assessed. The results showed no significant differences in growth and feeding parameters between control and MOS supplementation diets (P>0.05). The highest and the lowest growth performances were observed in 4.5 g kg -1 MOS and control group (P<0.05). Subsequently, immune responses (Ig levels, lysozyme activity and ACH50) were significantly higher in 4.5 g kg -1 MOS fed fish (P<0.05). The survival rate of control group was lower than the MOS groups, but not significant (P>0.05). These results indicate that MOS at 4.5 g kg -1 of the diet can be considered as a beneficial dietary supplement for improving the growth performance and immune response of gibel carp juveniles.