Expression of beta-tubulin during dormancy induction and release in apical and axillary buds of five woody species (original) (raw)

Cell cycle activity was studied in apical and axillary buds of growing plant material, whereas in dormant buds the accumu-Norway maple (Acer platanoides L.), apple (Malus 'M9'), lation was much lower or below detection level. It was obpedunculate oak (Quercus robur L.), Scots pine (Pinus syl7es-served for all species that during dormancy induction the tris L.) and rose (Rosa corymbifera 'Laxa') during dormancy amount of beta-tubulin decreased, while during dormancy release a fast accumulation of beta-tubulin occurred. The induction and release. Flow cytometric analyses revealed that in dormant buds, cells mainly were quiescent at the G 0 /G 1 dynamics of the beta-tubulin accumulation reflected the dormancy status of tree buds of the five species studied suggesting phase, while in non-dormant buds, a significantly higher frequency of G 2 cells was found in all species. In western blots that the beta-tubulin level might be useful as a marker for the dormancy status in buds of temperate woody species. accumulation of 55 kDa beta-tubulin was found in active