A recombinant fungal chitin deacetylase produces fully defined chitosan oligomers with novel patterns of acetylation (original) (raw)

Applied and environmental microbiology, 2016

Abstract

Partially acetylated chitosan oligosaccharides (paCOS) are potent biologics with many potential applications, and their bioactivities are believed to be dependent on their structure, i.e. their degrees of polymerization and acetylation as well as their pattern of acetylation. However, paCOS generated via chemical N-acetylation or de-N-acetylation of GlcN or GlcNAc oligomers, respectively, typically display random patterns of acetylation, making it difficult to control and predict their bioactivities. In contrast, paCOS produced from chitin deacetylases (CDA) acting on chitin oligomer substrates may have specific patterns of acetylation, as shown for some bacterial CDA. However, compared to bacterial CDA, we know little about the ability of fungal CDA to produce defined paCOS with known patterns of acetylation. Therefore, we optimised the expression of a chitin deacetylase from the fungus Puccinia graminis f.sp. tritici in E. coli The best yield of functional enzyme was obtained as a...

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