Growth of osteoblasts on porous calcium phosphate ceramic: an in vitro model for biocompatibility study (original) (raw)
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Journal of Bioscience and Bioengineering, 2000
The effects of biocompatible ceramics on the growth and adhesion of osteoblast-rich rat calvarial cell cultures were investigated. Osteoblast-lie cells and mouse fibroblast-like L-929 cells were cultured on composite sinters of hydroxyapatite (HAP) and @ricalcium phosphate (TCP) culture carriers, whose Ca/P molar ratios were adjusted to values of 1.50,1.55,1.60,1.64 and 1.67. The growth rates of both cell types were accelerated on the TCP-HAP ceramics as compared to those on polystyrene plastic (LUX) or bioinert zirconia ceramics. The population of osteoblast-like cells reached a density of 2.28 X 105 cells/cm2 on 100% HAP (Ca/P ratio 1.67) at 9 d of culture, while the corresponding cell density was 1.66 X lo5 cells/cm2 on LUX and 1.26 X 105 cells/cm2 on zirconia. Adhesion of the osteohlast-lie cells on TCP-I-L4P ceramics was similarly increased as compared with that on LUX or zirconia ceramics. The adhesion of L-929 cells on TCP-HAP ceramics was found to be weaker than that on cultures on LUX or zirconia ceramics. The time-dependent variations in the alkaline phosphatase activity of the osteoblast-like cells showed that the osteoblastic phenotype was potentiated by culturing the cells in calcium-rich media. The surface analyses of the Ca/P ratio and the microstructure by XRD and J3TIR suggest that the Ca-rich surface was newly formed by recombination on the surface layer in the culture medium containing fetal bovine serum. These results suggest that the surface of TCP-HAP ceramics, especially that of 100% HAP ceramics, are effective for accelerating growth and differentiation of osteoblast-like cells. This is most probably due to the chemical and physical instability and composition of 100% HAP, which promote the formation of a Ca-rich layer at the cell-material interface and provision of Ca ions to the osteoblast-like cells.
Trends in Biomaterials and Artificial Organs
We studied the in vitro levels of calcium, phosphate, and alkaline phosphate (ALP) activity in media of rat osteoblasts grown in the presence of various implant materials. Osteoblast cells from newborn Sprague-Dawley rats were harvested from calvaria. Cells were cultivated in the presence of Cobalt-Chrome (Co-Cr), cp Titanium (Ti), polymethylmethacrylate (PMMA), and wood in an artificial medium in a humidified atmosphere (5% CO 2) at 37 0 C. Cellular function was monitored over two weeks. Statistical tests (2-way ANOVA) of the results indicated that changing the disc material did not have a significant effect (p>0.05) on the amounts of Ca 2+ and PO 4 2-producedbut it did on the level of ALP activity in the medium (p<0.05). The culture time impacted significantly (p<0.05) on the levels of all 3 markers. Further, the interaction effect between the disc material and culture time was significant (p<0.05) for all markers. Wood produced the highest activity for ALP. Electron M...
Biomaterials, 2004
Calcium phosphate ceramics with different hydroxyapatite (HA) and tricalcium phosphate (TCP) ratios have different chemical properties. Does the difference in phase composition affect osteoblast behavior? In this study, osteoblasts were cultured on 4 kinds of calcium phosphate ceramics, i.e. pure (HA), HT1 (HA/TCP, 70/30), HT2 (HA/TCP, 35/65), and pure TCP. Cell proliferation of SaOS-2 cells together with bone-related genes' mRNA expression and protein production in osteoblasts cultured on different calcium phosphate ceramics were detected at different time points. Data suggested that cell proliferation rate on TCP ceramics was lower than that on the other substrates tested. Generally, mRNA expressions for ostenectin and osteocalcin were similar among the four kinds of ceramics in most circumstances, whereas at six days, alkaline phosphatase mRNA expression was higher on HA and HT1 surfaces than on the other two materials. Collagen I mRNA expression was also affected by the phase composition of substrates. Osteocalcin and bone sialoprotein production in SaOS-2 cells was very similar no matter which ceramic surface the cells were grown upon. This study revealed that calcium phosphate ceramics substrate could support osteoblast growth and bone-related gene expression and its gene expression pattern explained the basis of the biocompatibility and bioactivity for calcium phosphate ceramics. r
Osteoblast Cell Response to Naturally Derived Calcium Phosphate-Based Materials
Materials (Basel, Switzerland), 2018
The demand of calcium phosphate bioceramics for biomedical applications is constantly increasing. Efficient and cost-effective production can be achieved using naturally derived materials. In this work, calcium phosphate powders, obtained from dolomitic marble and seashells by a previously reported and improved Rathje method were used to fabricate microporous pellets through cold isostatic pressing followed by sintering at 1200 °C. The interaction of the developed materials with MC3T3-E1 pre-osteoblasts was explored in terms of cell adhesion, morphology, viability, proliferation, and differentiation to evaluate their potential for bone regeneration. Results showed appropriate cell adhesion and high viability without distinguishable differences in the morphological features. Likewise, the pre-osteoblast proliferation overtime on both naturally derived calcium phosphate materials showed a statistically significant increase comparable to that of commercial hydroxyapatite, used as r...
OR 67 Osteoconduction of calcium phosphate thin film on porous implants in rabbit
Journal of Endodontics, 1997
The author has granted a nonexclusive licence allowing the National Library of Canada to reproduce, loan, distri'bire or sell copies of this thesis in microform, paper or electronic formats. The author retains ownership of the copyright in this thesis. Neither the thesis nor substantial extracts fiom it rnay be printed or otherwise reproduced without the author's permission. the implant surface and thicker in the neck regions (in excess of 1.5 CM). This CaP layer, being thicker than the intended 0.3 Fm, often resulted in surface cracking and delamination in the neck regions. When the extent of bone ingrowth into the neck regions of both implant types in Experiments 1 & II were investigated, the Ca-Pcoated implants exhibited sigoificantiy @ < 0.0001) greater bone ingrowth into these neck regions than the control implants suggesùng a superior three-dimensional bone / implant interlock. The slight differences between group I & II sumzest the need for M e r investigation includine a thorough characterization of the sol-gel coating. Nevertbeless, the results indicate that an ultrathin laver of CaP may be capable of ~romotine; osteoconduction and the sol-gel technicrue of CaP a~~lication is suitable for Doroussurfaced imdant without occluding the pores.
Osteoinduction by calcium phosphate biomaterials
Journal of Materials Science-materials in Medicine, 1998
Different materials were implanted in muscles of dogs to study the osteoinduction of calcium phosphate biomaterials. Bone formation was only seen in calcium phosphate biomaterials with micropores, and could be found in hydroxyapatite (HA) ceramic, tricalcium phosphate/hydroxyapatite ceramic (BCP), β-TCP ceramic and calcium phosphate cement. The osteoinductive potential was different in different materials. The results indicate that osteoinduction can be a property of calcium phosphate biomaterials when they exhibit specific chemical and structural characteristics. © 1998 Kluwer Academic Publishers
Dental Materials Journal, 2005
Effects of functionally gradient calcium phosphate consisting of hydroxyapatite (HAP) and a-tricalcium phosphate (a-TCP) on proliferation and differentiation of osteoblasts were evaluated using MC3T3-E1 cells. There were no significant differences in the proliferation of MC3T3-E1 cells among HAP-a-TCP functionally gradient calcium phosphate, pure HAP, and cell culture plastic wells. mRNA expressions of type I collagen, alkaline phosphate, and osteocalcine were evaluated as indexes of initial, mid-stage, and late-stage osteoblastic differentiation. Basically, HAP-a-TCP functionally gradient calcium phosphate and pure HAP enhanced the expressions of the three markers when compared with that of cell culture plastic wells. For type I collagen and alkaline phosphate expressions, HAP-a-TCP functionally gradient calcium phosphate showed the same expression level as pure HAP. For osteocalcine expression, HAP-a-TCP functionally gradient calcium phosphate showed a higher level than pure HAP. We concluded, therefore, HAP-a-TCP functionally gradient calcium phosphate has good potential to be a bone filler material with high osteoconductivity.
Osseous substance formation induced in porous calcium phosphate ceramics in soft tissues
Biomaterials, 1994
Porous calcium phosphate ceramics prepared according to the methods of laboratories in Leiden were implanted subcutaneously in rats for 3, 6 and 12~. No bone formation could be observed. Porous calcium phosphate ceramics prepared according to the methods of laboratories in Sichuan were implanted sub~utaneously/intramusculariy in dogs for 1, 3, 5 and 7 months. In particular, ceramics containing cr-tricalcium phosphate evoked bone formation in the pores of the ceramics. The amount of bone was similar at the different time periods.
Acta veterinaria, 2008
Calcium phosphate ceramics are among the more commonly used and biocompatible ceramics. Recently, we have synthesized a new calcium phosphate ceramic, alpha tricalcium phosphate (aTCP). The aim of this study was to assess the biocompatibility of this original, in our laboratory modified, newly synthesized aTCP ceramic, by carefully evaluating the inflammatory reaction of soft tissue in response to its subcutaneous implantation, and by comparing this result with the results of already widely used virtually non-toxic, non-immunogenic, and almost chemically inert dental (DC) and hydroxy apatite (HAP) ceramics. Implants (diameter 5x2 mm) of aTCP, DC and HAP were implanted into 12 adult male rats subcutaneously. At 2 weeks and 12 weeks post-implantation, the animals were euthanized and the tissueimplant reactions were analyzed histologically. Evaluation of routine stained sections (5 mm, hematoxylin & eosin) of the cutis and subcutis surrounding the aTCP, DC and HAP ceramics revealed the following: 1) all the ceramic devices had fibrous connective tissue capsules; 2) there were significant differences in the tissue-implant reactions based on the estimated time, while there were no differences in the tissue-implant reaction based on the type of ceramic material; 3) two weeks after implantation hyperemia and cellular proliferation were the most expressive results, while twelve weeks after implantation extensive angiogenesis and collagen fibers production were the prominent findings for all types of implanted calcium phosphate ceramics.
Osteoblast and Fibroblast Culture Proliferation on Injectable Calcium Ceramics Polymer Composite
Bioceramics Development and Applications, 2011
Considerable demand for the repair of bone defects cannot be met solely by using biological donor materials. Hence, the use of biocomposites will most likely be increased in reconstructive surgery in future. Metallic implants cannot be used in all cases, because the defects in the skeleton vary greatly, differing from each other in their shape, size, anatomic location, and physiological weight-bearing in the anatomic location. Therefore, more sophisticated orthopaedic materials (e.g. non-metallic fibre-reinforced composites and particulate filler composites) should be available for clinical practice. Our studies have focused on the development of injectable composites of biostable bone cements, i.e. in situ curable resin systems containing impregnated calcium ceramics particles. The properties of the bone cement composites aspire to simulate as closely as possible the mechanical and structural properties of bone. The purpose of this study was to evaluate the in vitro cell proliferation on the experimental injectable biostable polymer modified with calcium ceramics. In the course of proliferation, the cell activity on the calcium ceramics containing biostable polymer composite increased throughout the experiment. As a conclusion, this cell proliferation study indicated that the studied biocomposite has a good potential to promote cell interaction.