Original Research Article Optimization and production of Polyhydroxybutarate(PHB) by Bacillus subtilis G1S1from soil (original) (raw)
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Polyhydroxyalkanoates (PHAs), which is produced by several bacteria, is a biodegradable polymer that has many industrial and medical applications. The present study deals with the isolation, and characterization of biopolymer (PHB) producing bacteria from the agricultural soil. The biopolymer producing bacteria was isolated from soil samples like crop, nursery, and fodder fields. The bacteria were screened by morphological and biochemical characteristics. The bacteria were further identified by Sudan black- B staining and molecular characterization. High and efficient PHB accumulation bacteria was selected by quantification and extraction method. Further bacteria were confirmed by FTIR analysis to know the functional groups present in the bacteria. A total of six bacillus species were isolated, the strain BBKGBS12 and BBKGBS6 are gram positive and other isolated bacteria were gram negative. Black colour granulesof bacteria BBKGBS6 were seen by Sudan Black-B staining. Also granules o...
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Poly-β-hydroxybutyrate (PHB) is the intracellular lipid reserve accumulated by many bacteria. The most potent terrestrial bacterium Bacillus cereus SE-1 showed more PHB accumulating cells (22.1 and 40% after 48 and 72 h) than that of the marine Bacillus sp. CS-605 (5 and 33% after 48 and 72 h). Both the isolates harbored phbB gene and the characteristics C=O peak was observed in the extracted PHB by Fourier transformed infrared spectroscopy analysis. Maltose was found to be the most suitable carbon source for the accumulation of PHB in B. cereus SE-1. The extracted PHB sample from B. cereus SE-1 was blended with a thermoplastic starch (TS) and an increased thermoplasticity and decreased crystallinity were observed after blending in comparison to the standard PHB. The melting temperature (T m ), melting enthalpy (ΔHf), and crystallinity (X c ) of the blended PHB sample were found to be 109.4°C, 64.58 J/g, and 44.23%, respectively.
Nepal Journal of Biotechnology, 2019
Polyhydroxybutyrates (PHBs) are energy reserves synthesized by different micro-organisms such as Alcaligenes, Pseudomonas, Staphylococcus, Algae, in excess of carbon and limitation of nutrients like nitrogen. These biopolymers are suitable alternate to synthetic carbon-based polymers. However, the high production cost limits their commercialization. The aim of this study was thus, focused on optimization of culture condition for maximum PHB production in an attempt to reduce the production cost. The micro-organisms for this purpose were isolated from 4 different soil samples and screened for PHB production. Culture conditions for these organisms were optimized by changing the parameters, viz., incubation time, pH, carbon source and NaCl concentration. Thus, optimized culture condition was used to culture the isolates for extraction of PHB and its analysis. The extracted compounds on FTIR-analysis gave characteristic C=O peak of PHB, thus, confirming the seven isolates to be PHB prod...
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Plastics produced from petrochemical sources and known as polypropylene are now accumulating in our environment at rates of millions of tons per year creating severe problems. The present study aims to the production and isolation of PHB (polyhydroxy-buty rate), a biodegradable plastic, from agro-industrial waste products (whey and date molasses) due to its high economic and industrial importance , taking into consideration many points that lead to produce PHB on large scale. The methodology of this study includes screening study for the isolation of a promising microbial producer of PHB , and optimization experiments to evaluate the best environmental and physiological factors that lead to maximum PHB production. Under the optimized conditions, Lactobacillus acidophilus has shown maximum production when grown for 4 days on date molasses supplemented with NB yielding 0.412g/50ml of PHB, followed by Bacillus thuringiensis (0.367g/50ml) grown for 4 days on the same medium, and Bacillus subtilis (0.337g/50ml) grown for 6 days on whey supplemented with glucose, yeast extract, and peptone. Eleven nutritional factors were examined for their significance on PHB production using a statistical design known as Plackette-Burman. Maximum PHB output of 43.1 g/l produced by Lactobacillus acidophilus was revealed by the statistical design, which represents about 7.04 fold increase in PHB production. Fedbatch fermentation was carried out using the optimized fermentation medium and PHB production has been increased to 27.5% as compared with batch closed process. PHB was detected by transmission electron microscopy and monitoring UV spectra of the sample by scanning the samples between 220 and 300nm compared with standard PHB. Lactobacillus acidophilus can be used for PHB production on large industrial scale, solving by this one of the problems of solid waste management that results from the accumulation of plastics and saving the environment from additional air pollution caused by its recycling.