Study of Some Gonadic Growth Index of Great Sturgeon (Huso Huso) Cultured in Brackish Water Condition (original) (raw)
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2012
A number of biochemical factors of blood (calcium, magnesium, total protein, cholesterol and glucose) were determined with weight and fork length of female fishes, biological characteristics of gonad (e.g. the number of eggs/gram, the number of larvae/gram, fluid egg rate, relative fecundity, gonadosomatic index, hatching success and fertilization rate) in 16 female of the migratory population of Persian sturgeon (Acipenser persicus) in Spring of the 2012. There was an invert correlation between weight and blood Mg +2 (P<0.05). Also, relationship between blood glucose and fluid egg rate, relative fecundity as well a s gonadosomatic index was reverse (p<0.05). These relationship was the same between glucose and the number of eggs/gram of gonad (p<0.05). Cholesterol was positively correlated with weight, fork length, the number of larvae/gram and hatching success but these correlations were not significant.
2008
An experiment was conducted on blood sexual hormone measurement of Great sturgeon, aged 4-5 years, cultured in brackish water in Bafgh-Iran constructions. Hormone levels were measured with Radio Immunoassay (RIA). Gonadic sampling was stained with Hematoxylin & Eosin. Statistical results show significant elevation of sexual hormones in both sexes (p>0.05; more in male). However, only estradiol had significant elevation among seasons (maximum in fall and minimum in winter). Testosterone (T) had significant elevation in relation to gonadic development, but in females, estradiol (E2) and progesterone (P) had not significant elevation. In males, II, II-III, III, IV stages, T level was 5.5, 4.4, 6.3, 2.6 ng/m respectively l. In females, II, II-III stage, T was 0.1, 0.16, E2 was 1.455, 0.81 and P was 0.12, 0.13 ng/ml respectively. We concluded that in females, with parallel to progression of gonadic development, T and P have increased but E2 have decreased. Results were in accordance t...
Bulgarian Journal of Agricultural Science
An experiment was conducted on blood sexual hormone measurement of Great sturgeon, aged 4-5 years, cultured in brackish water in Bafgh-Iran constructions. Hormone levels were measured with Radio Immunoas-say (RIA). Gonadic sampling was stained with Hematoxylin & Eosin. Statistical results show significant eleva-tion of sexual hormones in both sexes (p>0.05; more in male). However, only estradiol had significant elevation among seasons (maximum in fall and minimum in winter). Testosterone (T) had significant elevation in relation to gonadic development, but in females, estradiol (E2) and progesterone (P) had not significant elevation. In males, II, II-III, III, IV stages, T level was 5.5, 4.4, 6.3, 2.6 ng/m respectively l. In females, II, II-III stage, T was 0.1, 0.16, E2 was 1.455, 0.81 and P was 0.12, 0.13 ng/ml respectively. We concluded that in females, with parallel to progression of gonadic development, T and P have increased but E2 have decreased. Results were in accordance...
Twenty four farmed great sturgeon, Huso huso (including 8 males and 16 females) over 6 years old were used to develop a method for determination of sex and maturity stages. Seasonal gonadal tissue and blood samples were collected from farmed great sturgeon for three years. The sex and stages of maturity were determined by histology and laparoscopy at the beginning and end of experiment. Plasma sex steroid hormone levels [testosterone (T), 17β-estradiol (E2) and progesterone (P)] were measured by radioimmunoassay, and plasma calcium ion levels were measured by spectrophotometer. Mean concentrations of testosterone, progesterone and estradiol in blood plasma of H. huso at maturity stages II, III and IV were 10.86±1.63, 54.14±3.1, 112.41±7.4; 0.84±0.12, 15.66±2.18, 50.75±3.63 ng/ml in males and 9.0±1.39, 6.51± 0.64, 2.95±2.29, 5.45±0.29, 9.47±0.97 and 4.15±0.7 ng/ml in females, respectively. Testosterone and estradiol levels showed significant differences at various stages. Calcium level at stages II, III and IV of sexual maturity in females (8.05 ± 0.09, 10.4 ± 0.34 and 9.6 ± 0.6 mg/dl) was more than males (7.73 ± 0.16, 8.58 ± 0.13 and 8.76 ± 0.11 mg/dl). Results showed that steroid hormone concentration and calcium level of blood plasma in males and females vary between different stages of sexual maturity. Therefore it can be used to determine the stages of sexual maturity in farmed H. huso.
Iranian Journal of Fisheries Sciences, 2020
Gender and sexual maturity stages of great sturgeon (Huso huso) were studied using blood plasma sex steroids hormones ratios (17β-estradiol (E2), testosterone (T) and 11ketotestostrone (11KT)}. One hundred sixty reared great sturgeon aged (1-12 years) with weight and length 0.45 to 49.65 kg and 36-198 cm, respectively. For each gender, 80 individuals (20 fish in each maturity stage) were selected out of 650 fish. Hormone concentrations measured with commercial kits. All samples analyzed in duplicate and a separate standard curve run for each ELISA plate. The findings of this study showed that it is possible to separate male and female fish especially from the second sex maturation stage using T/E2 and 11KT/E2 hormone ratios. The results showed that the use of E2/T, E2/11KT ratios are very useful indices for sex determination and sexual maturity stages assessment in male reared great sturgeon, but T/11KT and 11KT/T ratios were not functional. Based on the results, the use of T/E2 and...
Сoncentration of Steriod Hormones and Ultrasonography of Pathologies of the Internal Organs of Females of the Siberian sturgeon Acipenser baerii (Acipenseridae) Reared at Constant Presence of Grower Feed and High Stocking Density, 2023
The hormonal and ultrasound screening have been performed for the females of Siberian sturgeon Acipenser baerii reared under continuous grower feed and high stocking density in a recirculating aquaculture system. Infertile females represented 33.3% of total number; they did not mature even at the age of 10 years, carrying the ovaries at the gonad maturation stage IIb (lipid) at the time of the study. The pathologies in liver were presented in 42.8% of such females together with abnormally high concentrations of androgens in the blood serum compared to normally maturing fish. Presumably, high stocking density (40 kg/m 2) and longterm supply with grower feed (protein content 45%, fat content 15%) were the main reasons reducing the reproductive characteristics of spawners and increasing the share of infertile and obese females. For the first time, the concentration of dehydroepiandrosterone sulfate (DHEA-S) has been used for screening of sturgeon breeding stocks; DHEA-S is a popular marker in medicine for the diagnosis of human female infertility against the background of high concentrations of androgens. Testing of immature Siberian sturgeon females using this hormone testified to a higher sensitivity of this method compared to testosterone measuring.
A number of biological parameters of gonad (e.g. the number of egg in one gram, relative fecundity, gonadosomatic index and absolute fecundity) and weight and total length of female fishes were determined with serum steroid hormones, testosterone (T), 17β-estradiol (E2) and progesterone (P) in 12 female of the migratory population of Common carp (Cyprinus carpio) in southeast of Caspian Sea during year from May 2011 and May 2012. A negative correlation of 17-β estradiol, progesterone and testosterone with absolute fecundity, relative fecundity and gonadosomatic index was observed in spring season but these correlates were not significant. Also in summer and autumn season a negative correlation of 17-β estradiol, progesterone and testosterone with relative fecundity and gonadosomatic index and a positive correlation with total weight and total length was observed. In autumn season there was a significant correlation between 17-β estradiol and total weight (P<0.05). Also in winter season, relationship between progesterone and total length was positive (P<0.05).
Aquaculture
In this study we investigated the effects of different times of reproductive migration on biochemical compounds fluctuation (calcium, magnesium, glucose, cholesterol, total protein, sodium and potassium) and on the percentage of haematocrit of coelomic fluid and on the fertilization rate of 14 Persian sturgeon brood stocks, during the spawning season (in March of 2008 until May of 2008). For this purpose, the period of sampling was divided to three groups, beginning (March), middle (April) and end (May) of spawning season of these fishes. The average of fork length and total weight of fishes before extraction of eggs of Persian sturgeon brood stocks in treatments 1, 2 and 3 were 166.5 ± 6.36, 156.71 ± 8.69 and 174.5 ± 2.12 cm, also 33.0 ± 1.41, 30.1 ± 7.19 and 34.5 ± 0.70 kg, respectively. On the other hand, the average of percentage of fertilization rate in treatments 1, 2 and 3 were respectively 166.5 ± 6.36, 156.71 ± 8.69 and 174.5 ± 2.12 cm, 33.0 ± 1.41, 30.1 ± 7.19 and 34.5 ± 0.70 kg , 67.5 ± 6.36, 68.3 ± 17.86 and 49.5 ± 3.53 percent. Our results showed that there were significant differences in calcium (5.41±0.51 -1.65±0.03 and 11.99±1.41 mg dL -1 ), glucose (37.99±15.10 -23.61±6.45 and 11.3±3.26 mg dL -1 ), cholesterol (15.55±3.79 -8.26±2.25 and 10.33±1.88 mg dL -1 ) and sodium (185.94±38.86 -103.98±31.78 and 180.75±6.47 mM L -1 ) between these treatments (P<0.05) but in the percentage of fertilization rate, haematocrit and other calculated biochemical compounds, there were no significant differences (P>0.05). Overall, significant and insignificant fluctuations in coelomic fluid in spawning season of these fishes demonstrated that ovarian fluid in treatments number 1 (March) and 3 (May) had better characteristics for sperm mobility compared to treatments 2 (April).
Danubian Animal Genetic Resources
Determination of the gender in sturgeon is very important in fish farmers, as sex is one of the main factors that determine aim of cultivating them. One of the method for maturation monitoring of sturgeons is steroid hormone analysis. On this base the current study used estradiol and testosterone hormones to determine the gender of 2-3-year-old Siberian sturgeon (Acipenser baerii) cultivated in sturgeon broodstock farm located in the Zhrebchevo Dam lake, Bulgaria. The serum concentrations of circulating reproductive hormones found in the both sex were similar to the hormonal profiles in previously reports. The results were confirmed also by histological examination, which showed pre-vitellogenic stage (Stage I) in female and maturity (Stage V) at the male individuals. The findings indicate the force of current steroid hormones as a method for sex determination. In conclusion, analysis of circulating reproductive hormones may be a useful method in early determining the sex of Siberia...
Effects of age and body size on gonadal development of Atlantic sturgeon
Journal of Fish Biology, 1998
Three hundred and five Atlantic sturgeon caught in the Hudson River and Hudson Bight were examined for age, body size and gonadal development (histology). Sampled fish ranged in age from 1·5 to 43 years and fork length (L F ) 48-244 cm, and included 144 females and 161 males. All young sturgeon (age 1·5-4 years) caught in the river had sexually differentiated gonads. Age and size distributions of subadults and adults at different stages of gametogenesis were sex-specific and conformed with von Bertalanffy growth curves. Males mature faster and at a younger age compared to the females. Females with mid-vitellogenic ovaries were not found and their absence may be related to fishing gear selectivity or out-migration into the ocean during ovarian recrudescence. Mature males and females caught during the spring spawning migration into the Hudson River, ranged in age from 12 to 19 and from 14 to 43 years, and L F 117-185 and 173-244 cm, respectively. Individual fecundity and diameter of fully grown ovarian follicles increased with age and body size, but this increase was dampened in older females. Three hermaphroditic fish were found and all exhibited sparse pre-vitellogenic ovarian follicles embedded in normally developing testicular tissue. 1998 The Fisheries Society of the British Isles