Estradiol and medroxyprogesterone acetate regulated genes in T47D breast cancer cells (original) (raw)

2005, Molecular and Cellular Endocrinology

Many mammary tumors express estrogen receptors (ER) and progesterone receptors (PR), and there is increasing evidence that progestins influence gene expression of breast tumor cells. To analyse the impact of progestins on breast cancer cells, we compared (a) the expression of two cytokines, involved in tumor progression, and searched (b) for differentially regulated genes by a microarray, containing 2400 genes, on T47D breast cancer cells cultured for 6 days with 17␤-estradiol (E 2 ) or E 2 + medroxyprogesterone acetate (E 2 + MPA). Lower amounts of PDGF and TNF␣ were found in culture supernatants of E 2 + MPA treated T47D cells. MPA addition induced a 2.8-3.5-fold increase of the mRNA expression of (a) tristetraprolin, which is involved in the posttranscriptional regulation of cytokine biosynthesis, and (b) zinc-␣2-glycoprotein and Na, K-ATPase ␣1-subunit, which both resemble differentiation markers of breast epithelium. In contrast, the mRNA expression of lipocalin 2, which promotes matrixmetalloproteinase-9 activity, was decreased five-fold in E 2 + MPA treated cells.