Novel Real-Time PCR Assays Using TaqMan Minor Groove Binder Probes for Identification of Fecal Carriage of Streptococcus bovis/Streptococcus equinus Complex from Rectal Swab Specimens (original) (raw)
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Zahedan Journal of Research in Medical Sciences, 2021
Objective: Different types of Streptococcus gallolyticus are associated with malignant colorectal cancer. This study aimed at scrutinizing the S. gallolyticus screening using polymerase chain reaction (PCR). Methods: A descriptive study was conducted to detect S. gallolyticus in 55 patients with colon diseases referring to hospitals in Babol and Chalus, Iran. PCR and culture techniques were performed. Detection of S. gallolyticus after DNA extraction from designed primers (PCO3 and PCO4) was done for SODA gene. Fisher's exact test was used for the analysis of PCR results. A P < 0.05 was considered significant. Results: Of 55 biopsy samples of patients with colon diseases, 3 samples (5.5%) with 95% confidence interval and 52 samples (94.5%) were reported negative in terms of DNA of S. gallolyticus. In the analysis of PCR gels, the remaining 406 bp band after performing PCR showed that all samples were in good condition considering DNA quality. Conclusion: Specific probes have ...
2010
Streptococcus bovis is a member of group D streptococcicommon inhabitants of the intestine and causative agents of endocarditis. A well-recognized association has been established between S. bovis endocarditis and colon carcinoma, which led to great interest in the identification of this organism [1]. The S. bovis group is divided into 3 biotypes according to their biochemical characteristics: biotype I (mannitol fermentation positive), biotype II/1 (mannitol negative and b-glucuronidase negative), and biotype II/2 (mannitol negative and b-glucuronidase positive) [2]. Each biotype has somewhat different pathogenicity. S. bovis biotype I has been documented to be the biotype more likely associated with both endocarditis and malignant or premalignant colonic lesions [3]. The taxonomy of the S. bovis group has been evolving in the last few decades, and a new nomenclature was adopted on the basis of genetic distances and phylogenetic analyses. The new classification lists S. bovis biotypes I, II/1, and II/2 as Streptococcus gallolyticus subsp. gallolyticus, Streptococcus infantarius
International Journal of Cancer Management, 2020
Background: Different types of Streptococcus gallolyticus are associated with malignant bowel cancer. Objectives: The aim of this study was to compare two culture and molecular methods in identifying Streptococcus gallolyticus in patients with colon diseases. Methods: A descriptive study was conducted to detect Streptococcus gallolyticus in 55 patients with colon diseases referring to hospitals in Babol and Chalus, Iran. A polymerase chain reaction and culture technique were performed. Detection of Streptococcus gallolyticus after deoxyribonucleic acid (DNA) extraction from designed primers (PCO3, PCO4) was used for SODA gene. From the general culture medium, brain heart infusion (BHI) broth and specific medium for bacterial growth and detection were used. Then, the characteristics of the two methods were evaluated. Results: Of 55 biopsy samples of patients with colon diseases, 3 samples (5.5%) with 95% confidence interval were positive and 52 (94.5%) were reported negative in terms...
FEMS Microbiology Letters, 2000
Streptococcus bovis has been identified as a causative agent in humans for a variety of diseases, including endocarditis, meningitis, and septicemia. Identification of S. bovis strains of human origin in clinical settings has been problematic due to variations in biochemical tests as compared to ruminal strains of S. bovis, and other streptococcal species. DNA-DNA hybridization with chromosomal DNA from various S. bovis strains indicates that strains of human origin are different from those of ruminal origin. Specific probes have been designed from S. bovis 16S rDNA gene sequences that differentiate strains of human and ruminal origin by direct hybridization and PCR analyses. These techniques now allow for rapid identification of S. bovis strains for clinical and other scientific investigations. ß
Journal of Clinical Microbiology, 2001
We characterized 22 human clinical strains of Streptococcus bovis by genotypic (16S rRNA gene sequence analysis [MicroSeq]; Applied Biosystems, Foster City, Calif.) and phenotypic (API 20 Strep and Rapid ID32 Strep systems (bioMerieux Vitek, Hazelton, Mo.) methods. The strains, isolated from blood, cerebrospinal fluid (CSF), and urine, formed two distinct 16S ribosomal DNA sequence clusters. Three strains which were associated with endocarditis urinary tract infection (UTI), and sepsis clustered with the S. bovis type strain ATCC 33317 (cluster 1); other closely related type strains were S. equinus and S. infantarius. Nineteen strains clustered at a distance of about 2.5% dissimilarity to the S. bovis type strain (cluster 2) and were associated with central nervous system (CNS) disease in addition to endocarditis, UTI, and sepsis. All strains were distinct from S. gallolyticus. Within cluster 2, a single strain grouped with ATCC strain 43143 (cluster 2a) and may be phenotypically distinct. All the other strains formed a second subgroup (cluster 2b) that was biochemically similar to S. bovis biotype II/2 (mannitol negative and beta galactosidase, alpha galactosidase, beta glucuronidase, and trehalose positive). The API 20 Strep system identified isolates of cluster 2b as S. bovis biotype II/2, those of cluster 1 as S. bovis biotype II/1, and that of cluster 2a as S. bovis biotype I. There was an excellent correlation of biotype and genotype: S. bovis biotype II/2 isolates form a separate genospecies distinct from the S. bovis, S. gallolyticus, and S. infantarius type strains and are the most common isolates in adult males.
Association between Streptococcus bovis and colorectal cancer among Libyan patients
Mediterr J Pharm Pharm Sci, 2021
Streptococcus bovis/gallolyticus was considered as lower grade pathogen involved in endocarditis. Recent accumulating evidence has suggested that Streptococcus bovis/gallolyticus plays an important role in the initiation and development of colorectal cancer. This study was aimed to address the association between Streptococcus bovis/gallolyticus and colorectal cancer among Libyan patients, to determine the dominant biotype of Streptococcus bovis/gallolyticus associated with colorectal cancer patients and to evaluate the antibiotic susceptibly patterns of isolated strains. The study involved 20 colorectal cancer patients, 20 patients with large adenomas polyp and 20 people with healthy colonic mucosa as a control group. All the candidates were subjected to colonoscopy and histopathological examinations and conformation. The suspected and normal cases were then diagnosed according to the protocol used by the medicine specialist and grouped in accord with the study aim. All biopsies were handled and treated to isolate the suspected positive colonies of Streptococcus bovis/gallolyticus. Of the 20 colorectal cancer patients, seven patients (35%) were found to be Streptococcus bovis/gallolyticus carriers, (95% CI 1.22-1.72, p < 0.05). Nine patients (45%) were diagnosed colorectal cancer positive at age above 60 years old, eight patients (40%) at age 40-60 years old and three patients (15%) at age bellow 40 years old. Also, nine out of 20 patients (45%) with large adenomas polyp were found to be Streptococcus bovis/gallolyticus carriers, (95% CI 2.16-2.71, p < 0.05). Of the 20 healthy people with colonic mucosa, Streptococcus bovis/gallolyticus never been isolated. Streptococcus. bovis biotype II/I and Streptococcus. equinis were both susceptible to the above mentioned antibiotic classes, although Streptococcus bovis biotype I was highly resistance to most clinically used antibiotics. However, there was strong relationship between Streptococcus bovis/gallolyticus and colorectal cancer or large polyp formation. However, it is not well understood whether the bacterium has a pathogenic role in the initiation and progression of neoplasia or just an epiphenomenon of colorectal neoplasms.
Journal of clinical microbiology, 1998
Strains formerly identified as Streptococcus bovis were allotted to two groups by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of whole-cell proteins. Strains from humans with infections, mostly patients with endocarditis, and strains from pigeons with septicemia clustered with the recently described species Streptococcus gallolyticus. The original S. bovis type strain and strains exclusively from ruminants formed the second cluster. The findings indicate that S. gallolyticus is more likely to be involved in human and animal infections than S. bovis. Growth characteristics and several biochemical reactions were found to be useful in the differentiation of S. gallolyticus from S. bovis.
Journal of Clinical Microbiology, 1998
Strains formerly identified as Streptococcus bovis were allotted to two groups by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of whole-cell proteins. Strains from humans with infections, mostly patients with endocarditis, and strains from pigeons with septicemia clustered with the recently described species Streptococcus gallolyticus. The original S. bovis type strain and strains exclusively from ruminants formed the second cluster. The findings indicate that S. gallolyticus is more likely to be involved in human and animal infections than S. bovis. Growth characteristics and several biochemical reactions were found to be useful in the differentiation of S. gallolyticus from S. bovis.
Streptococcus Bovis Fecal Carriage is Not Associated with an Increased Risk of Colorectal Neoplasm
2008
Purpose. The aim of the study is to evaluate the association between fecal isolates of Streptococcus bovis and colorectal neoplasms. Methods. From Dec 1993 to Dec 1995, 1,121 stool samples were obtained from 1,048 recruits including 62 with newly diagnosed colorectal neoplasms. Fecal isolates of S. bovis were identified with API 20 Strep System for both healthy (average risk) and colorectal cancer group. The demographic features, S. bovis fecal carriage status, colonoscopy and pathological findings of colonic lesions, if any, were collected. Medical records of the 986 average risk group subjects were retrieved to evaluate the incidence of colorectal neoplasm during the one-decade interval. Results. The S. bovis fecal carriage rate of the colorectal cancer group was significant higher than that of the average risk group (25.8% vs. 14.5%, p=0.015). The distribution of S. bovis biotypes did not show a statistical difference between the two groups (χ^2 test, p=0.774). Full colonoscopy w...