Inmunoexpresión de p53 en Carcinomas de Células Escamosas Oralesde Sitios Anatómicos Distintos: Un Estudio Comparativo (original) (raw)
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The Journal of Laryngology & Otology, 1999
The TNM staging system is helpful but not enough to determine prognosis of the patients with squamous cell carcinoma of the oral tongue. T-stage alone is not suggestive for prediction of occult nodal metastases. For this reason, histopathological examination of 70 patients with squamous cell carcinoma of the oral tongue was done retrospectively. The histological differentiation, tumour thickness, perineural and lymphovascular space invasions, the amount of lymphocyte infiltration and pattern of tumour invasion were examined. Immunohistochemical examination was used to determine p53 immunoreactivity as well. The effect of these histopathological parameters and p53 immunoreactivity on nodal metastases and locoregional recurrence were analyzed using the chi-squared test. In terms of nodal metastases the only statistically significant difference between the two groups was tumour thickness, either <9 mm or >9 mm (p<0.05, \ 2 = 17.182). Tumour thickness, perineural invasion, lymphovascular space invasion, the amount of lymphocyte infiltration all correlated statistically with locoregional recurrence (/?<0.05, x 2 = 6.293 for tumour thickness; p<0.06, p = 0.054 for perineural invasion; p<0.05, \ 2 = 8.689 for lymphovascular space invasion; p<0.05, x 2 = 5.320 for lymphocyte infiltration). The immunoreactivity of p53 correlated significantly with larger primary tumour size (p<0.05, x = 5.440, lymph node metastases (p<0.05, X = 4.093) and with pathological tumour stage (p<0.05, x 2 = 5.713).
Journal of medical sciences, 2022
Objective: To assess the immunohistochemical expression of p53 in tissue samples of oral squamous cell carcinoma (OSCC), oral epithelial precursor lesions, and normal oral mucosa. Material & Methods: A comparative cross-sectional study was jointly conducted at the Departments of Pathology and Oral and Maxillofacial Surgery of various medical and dental institutes of the country from April 2016 to March 2017. A total of 180 subjects were included in the study. Oral tissue specimens were collected for laboratory investigations after obtaining written consent from all subjects. p53 was assessed using immunohistochemistry in tissue samples of 60 cases of OSCC, 60 cases of epithelial precursor lesions, and normal oral mucosal samples of 60 healthy individuals. Data were recorded, evaluated, and analyzed by SPSS-20. Results: p53 protein expression was noted in 85% OSCC and 73% oral epithelial precursor lesions. Among healthy individuals, one subject showed p53 immunoreactivity in the normal oral mucosa. Conclusion: Raised p53 overexpression in OSCC and oral precursor lesions, compared to normal oral mucosa make it a probable candidate for a potential predictive biomarker in oral premalignancy and malignancy.
Significance of p53 expression in non-tumoral epithelium adjacent to oral squamous cell carcinomas
The Journal of Laryngology & Otology, 2002
Alterations of the p53 gene are the most frequently documented genetic abnormalities in human cancer. The aim of the present study was to analyse if this alteration is an early event in oral tumorigenesis and if the suprabasal expression of p53 is a marker of the presence and severity of epithelial dysplasia. Immunohistochemical p53 expression in 78 specimens of oral squamous cell carcinoma and non-tumoral adjacent epithelium was analysed. Non-tumoral epithelium was observed in 53 cases (67.9 per cent), being normal in six cases (7.6 per cent), hyperplastic in 24 cases (30.7 per cent) and dysplastic in 48 cases (61.5 per cent). Epithelial dysplasia was mild (23 cases, 47.9 per cent); moderate (23 cases, 47.9 per cent) and severe (two cases, 4.1 per cent). Twenty-one cases of the dysplasias (43.8 per cent) expressed p53. No p53 expression appeared in any normal epithelium. Basal p53 expression always appeared in mild dysplasias (two cases). Suprabasal p53 expression appeared in mild and moderate dysplasias in nine cases and in one severe dysplasia. No statistical correlation was observed between suprabasal expression of p53 and the presence or severity of the dysplasia. The expression of p53 is an early event in oral tumorigenesis but it does not behave as an objective marker of the presence or severity of epithelial dysplasia.
Immune-Expression of P53 in Oral Preneoplastic and Neoplastic Squamous Lesions
2019
Objective: This study aimed to detect p53 immuno-expression in oral preneoplastic and neoplastic squamous lesions and to evaluate the correlation between these lesions and p53. Methodology: Hundred cases of Oral Squamous Cell Carcinoma (OSCC) and 50 cases of Oral Premalignant Lesions (OPL) were included. Selected tissues were stained with Hemotoxylin & Eosin (H&E), followed by immunohistochemistry for p53. Results: Nearly half (48%) of OSCC were well differentiated, 37% moderately, and 15% were poorly differentiated. Majority (74%) of the presented cases were in advanced stages. In OPL cases, 50% presented as mild dysplasia, 32% as moderate, and 18 % as severe dysplasia. p53 nuclear staining was positive in 70 (70 %) cases of OSCC and 27 (54 %) of OPL. Correlation of p53 with tumor histological grade was significant (P<0.05). Conclusion: High frequency and strong positivity of p53 tumor suppressor gene in oral dysplasia and OSCC have been implicated as an early indicator in oral ...
Asian Pacific Journal of Cancer Prevention, 2016
Oral tongue squamous cell carcinoma (OTSCC) is the most common oral cancer subtype with a maximum propensity for regional spread. Our objective was to study if p53 expression might have any correlation with aggressive patterns of invasion within oral tongue cancers as well as with the histologically identified degree of oral tongue dysplasia. p53 immunoexpression was studied using immunohistochemistry in early staged OTSCCs (n=155), oral tongue dysplasias, (n=29) and oral tongue normal specimens (n=10) and evaluated for correlations with histological and clinicopathological parameters. Our study (n=194) showed a pattern of p53 expression increasing with different grades of tongue dysplasia to different grades of invasive OTSCC (p=0.000). Among the OTSCC tumours, positive p53 expression was seen in 43.2% (67/155) and a higher p53 labelling index was significantly associated with increased Bryne's grade of the tumour invasive front (p=0.039) and increased tumour depth (p=0.018). Among the OTSCC patients with tobacco habits, (n=91), a higher p53 labelling index was significantly associated with increased risk of local recurrence (p=0.025) and with lymphovascular space involvement (p=0.014). Evaluation of p53 through varying degrees of dysplasia to oral tongue cancer indicates that p53 expression is linked to aggressive features of oral tongue cancers and tongue precancers entailing a closer monitoring in positive cases. Among the OTSCCs, p53 expression is associated with tumour aggressiveness correlating with increased grading of invasive tumour front and tumour depth.
Oral Oncology, 1997
The relationship between the histological grading of malignancy and ~53 protein expression was studied in 40 biopsies of oral squamous cell carcinomas. An immunohistochemical analysis was carried out using the steptavidin method preceded by a treatment with citric acid in a microwave oven. All cases were classified according to the histological malignancy grading system proposed by Anneroth et al. (Scandinavian Journal of Dental Research 1987, 95, 229-249). The expression of ~53 was found in 62.5% of the carcinomas studied. Positivity of ~53 staining showed a correlation with the histological grade of malignancy and with the degree of keratinisation, nuclear polymorphism and number of mitoses. .
IP Innovative Publication Pvt. Ltd., 2017
Introduction: Oral squamous cell carcinoma (OSCC) is the sixth most common cancer in the world. It usually develops in males in the 6th and 7th decade and is caused by tobacco and alcohol consumption. Materials and Method: This study was performed on 40 histopathologically proven cases of squamous cell carcinoma of oral region in Department of Pathology SGRDIMSR, Amritsar, Punjab. Tissue were stained for H & E and further immunostained for p53 receptor. p53 expression was correlated with histological grading, age, sex, vascular, perineural, muscle invasion and lymph node metastasis. Results: In our study, maximum incidence was seen in age group of 41-60 years with a male:female ratio of 7:1. A statistically significant correlation between p53 positivity with grade of carcinoma and cases presenting with nodal metastasis was seen. However, no such correlation was seen with age, sex, vascular, neural and muscle invasion. Conclusion: p53 positivity shows a direct correlation with grade and lymph node metastasis of SCC in head and neck. Thus, p53 has an influence on prognosis.
P53, p16 and Ki67 immunoexpression in oral squamous carcinomas
Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie, 2012
In this study, we have analyzed clinically, histopathologically and immunohistochemically a total of 34 cases of oral squamous carcinoma in 11 of the cases being identified adjacent epithelial dysplastic lesions. Carcinomas were diagnosed in patients aged 40-60 years, males, with chronic exposure to tobacco and/or alcohol, being located especially on the lips. Well-differentiated carcinomas have been predominant (52.9%) in stage I/II tumoral (88.3%). Immunoexpression analysis of p53, p16 and Ki67 did not reveal statistically significant differences between the expression of markers and clinical or histopathological parameters, except Ki67 whose increased expression was associated to the decrease of the degree of tumoral differentiation and with high degree dysplasia. The positivity index and the intensity of reaction were increased at the level of dysplasic epithelium for p16 and at the level of tumoral invasion front for the p53 and Ki67. The study highlights the value of the immun...
European Journal of Translational Myology
Mutations in tumor suppressor p53 protein can occur at different phases of malignant transformation and affect the patient's prognosis. This study aimed to evaluate the expression of mutant p53 protein in Iranian patients with the primary and recurrence oral squamous cell carcinoma (OSCC). This retrospective cross-sectional study conducted on a group of patients with the primary OSCC (n=122) and the control subjects with oral noncancerous reactive lesions (n=80). Immunohistochemistry was performed with the DO-7 monoclonal antibody against p53 protein, and samples with ≥10% immunostaining were considered positive. Statistical analyses were carried out using SPSS. Positive staining for p53 was observed in none of the control subjects and 57.4% (70 of 122) of the primary OSCC patients (p<0.0001, OR=107.69, 95%CI=6.49-179.0). The p53 immunopositivity had no significant differences between males and females (54.2% vs. 62%, p=0.390), but significantly different between those aged b...