Development and Validation of RP-LC Method for Simultaneous Estimation of Amlodipine Besylate and Valsartan in Bulk and Its Pharmaceutical Formulations (original) (raw)

The developed new method for the simultaneous estimation of Amoldipine besylate and Valsartan in tablet formulations is simple, rapid, selective, precise and accurate .The present work has been developed by non isocratic reverse phase high performance liquid Chromatography assay method. The separation was carried out by using column Symmetry C18, (150 x 4.6 mm, 3.5 µ) (Make: Waters), in mobile phase consisted of Acetonitrile and pH 3.0 triethylamine buffer. The flow rate was 1.0 mL/min, column oven temperature 35 oC, the injection volume was 10 μL, and detection was performed at 237 nm using a photodiode array detector (PDA), Run time 20 min. The retention time of amlodipine besylate and valsartan, was noted to be 5.2 min and 8.5min respectively, indicative of rather shorter analysis time. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent. Validation of RP-LC Method for Simultaneous Estimation of Amlodipine Besylate and Valsartan in Bulk and Its Pharmaceutical Formulations,