NNP010 Effects of exogenously induced fever and hyperthermia on brain damage in the immature rat (original) (raw)
2007, European Journal of Paediatric Neurology
Supraphysiological oxygen concentrations exert significant toxicity to the developing lungs and retina. However, little information is known about the effects of supraphysiological oxygen concentrations on the developing brain. The aim of this study is to investigate the effects of hyperoxia on prefrontal cortex of the developing brain. Effects of hyperoxia were studied by exposing Wistar pups (n = 8) from birth until postnatal day 5 to 80% oxygen in a chamber controlled by an oximeter. Control animals (n = 8) were maintained in room air (21% oxygen). Cell death was assessed with ELISA cell death assay, neuron counting, and terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) reaction. There was a statistically significant difference in regard to the neuronal density of the prefrontal cortex between the study and control groups (20.37±1.40 versus 26.25±2.37; p = 0.001). There was also a significant increase in ELISA cell death assay in the prefrontal cortex after five day exposure to hyperoxia (10.40±0.83 versus 9.19±0.48 absorbance gram protein; p = 0.001). Our results show that exposure of the developing brain to high concentrations of oxygen causes neuronal loss and an apoptotic neurodegenerative reaction in the prefrontal cortex of the rats.
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