One-pot labeling and purification of peptides and proteins with fluorescein maleimide (original) (raw)

A systematic study of protein labeling by fluorogenic probes using cysteine targeting vinyl sulfone-cyclooctyne tags

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Organic and Biomolecular Chemistry, 2016

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A fast and inexpensive method for N-terminal fluorescein-labeling of peptides

Gerd Folkers

Bioorganic & Medicinal Chemistry Letters, 1998

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Fluorescent labeling of tetracysteine-tagged proteins in intact cells

Mark Ellisman

Nature Protocols, 2010

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Efficient site-specific labeling of proteins via cysteines

Natalie Gassman

Bioconjugate chemistry, 2008

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Site-specific Fluorescent Labeling of Poly-histidine Sequences Using a Metal-chelating Cysteine

Lila Gierasch

Chemical Biology & Drug Design, 2007

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A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification†Electronic supplementary information (ESI) available: LC-MS, ES-MS, deconvoluted spectra and fluorescence emission spectra for all reactions with proteins described herein. Fluorescence emi...

Vijay Chudasama

Chemical science (Royal Society of Chemistry : 2010), 2013

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Rapid and high-yielding cysteine labelling of peptides with N-succinimidyl 4-[18F]fluorobenzoate

Santiago Rojas

Chemical Communications, 2012

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Single-label fluorescent derivatization of peptides

Michael Harvey

Analytica Chimica Acta, 1997

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Site-specific near-infrared fluorescent labelling of proteins on cysteine residues withmeso-chloro-substituted heptamethine cyanine dyes

Franck Denat

Organic & Biomolecular Chemistry, 2018

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Methods for the carboxyl-terminal fluorescent labeling of peptides using solid phase peptide synthesis

William Stern

Tetrahedron Letters, 1989

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Saturation fluorescence labeling of proteins for proteomic analyses

John E Wiktorowicz

Analytical Biochemistry, 2008

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Thiol-reactive dyes for fluorescence labeling of proteomic samples

John E Wiktorowicz

ELECTROPHORESIS, 2003

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Purification of Tetracysteine-Tagged Proteins by Affinity Chromatography Using a Non-Fluorescent, Photochemically Stable Bisarsenical Affinity Ligand

Bruce Branchaud

Bioconjugate Chemistry, 2011

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Fluorescence labeling, purification, and immobilization of a double cysteine mutant calmodulin fusion protein for single-molecule experiments

Todd D Williams

Analytical Biochemistry, 2004

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Probing the cysteine 34 residue in human serum albumin using fluorescence techniques

Toru Maruyama

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1997

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Novel Peptide Sequence (“IQ-tag”) with High Affinity for NIR Fluorochromes Allows Protein and Cell Specific Labeling for In Vivo Imaging

Jonathan Carson

PLOS One, 2007

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A facile method to prepare C-terminal fluorescently labelled peptides by an Fmoc strategy

Michael Pennington

Letters in Peptide Science, 1994

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Fluorescent Labeling Techniques in Biomolecules: A Flashback

Harekrushna Sahoo

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Efficient cysteine labelling of peptides with N-succinimidyl 4-[ 18F]fluorobenzoate: Stability study and in vivo biodistribution in rats by positron emission tomography (PET)

Santiago Rojas

2013

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Engineered fluorescence tags for in vivo protein labelling

Xiaozhe Ding

RSC Advances, 2014

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Fluorescent probes and bioconjugation chemistries for single-molecule fluorescence analysis of biomolecules

Shimon Weiss

The Journal of Chemical Physics, 2002

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Heterogeneity of protein labeling with a fluorogenic reagent, 3-(2-furoyl)quinoline-2-carboxaldehyde

Hossein Ahmadzadeh

Journal of Chromatography B, 2002

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An Affinity-Based Method for the Purification of Fluorescently-Labeled Biomolecules

trung nguyen

Bioconjugate Chemistry, 2006

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Site-Specific Incorporation of Fluorescent Probes into Protein: Hexahistidine-Tag-Mediated Fluorescent Labeling with (Ni 2+ :Nitrilotriacetic Acid) n −Fluorochrome Conjugates

Richard Ebright

Journal of the American Chemical Society, 2001

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Cysteine-reactive covalent capture tags for enrichment of cysteine-containing peptides

Loïc Dayon, Nikolett Mihala

Rapid Communications in Mass Spectrometry, 2009

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Fluorescent Amino Acids: Modular Building Blocks for the Assembly of New Tools for Chemical Biology

Andrew Krueger

ChemBioChem, 2013

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