Pathways of reducing equivalents in hepatocytes from starved, ethanol-induced, and hyperthyroid rats during ethanol and xylitol metabolism (original) (raw)

The metabolism of xylitol was studied in the absence or presence of ethanol in cells from 24-h-starved rats, either untreated, Ts treated, or ethanol induced. The relative yields of tritium in water, glucose, and lactate were determined. A simple metabolic model is proposed, from which the specific radioactivity of cytosolic NADH, flux through lactate dehydrogenase, and the relative yield of tritium in water and glucose can be calculated. The model assumes one cytosolic NAD pool and metabolic and isotopic steady state. The measured incorporation of tritium into glucose indicate that the 4A-and 4Bhydrogens of NADH are not fully equilibrated.