Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands (original) (raw)
Related papers
Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., Isolated from Sea Water
PLoS ONE, 2014
Two non-pigmented, motile, Gram-negative marine bacteria designated R9SW1 T and A3d10 T were isolated from sea water samples collected from Chazhma Bay, Gulf of Peter the Great, Sea of Japan, Pacific Ocean, Russia and St. Kilda Beach, Port Phillip Bay, the Tasman Sea, Pacific Ocean, respectively. Both organisms were found to grow between 4uC and 40uC, between pH 6 to 9, and are moderately halophilic, tolerating up to 20% (w/v) NaCl. Both strains were found to be able to degrade Tween 40 and 80, but only strain R9SW1 T was found to be able to degrade starch. The major fatty acids were characteristic for the genus Marinobacter including C 16:0 , C 16:1 v7c, C 18:1 v9c and C 18:1 v7c. The G+C content of the DNA for strains R9SW1 T and A3d10 T were determined to be 57.1 mol% and 57.6 mol%, respectively. The two new strains share 97.6% of their 16S rRNA gene sequences, with 82.3% similarity in the average nucleotide identity (ANI), 19.8% similarity in the in silico genome-to-genome distance (GGD), 68.1% similarity in the average amino acid identity (AAI) of all conserved proteincoding genes, and 31 of the Karlin's genomic signature dissimilarity. A phylogenetic analysis showed that R9SW1 T clusters with M. algicola DG893 T sharing 99.40%, and A3d10 T clusters with M. sediminum R65 T sharing 99.53% of 16S rRNA gene sequence similarities. The results of the genomic and polyphasic taxonomic study, including genomic, genetic, phenotypic, chemotaxonomic and phylogenetic analyses based on the 16S rRNA, gyrB and rpoD gene sequence similarities, the analysis of the protein profiles generated using MALDI-TOF mass spectrometry, and DNA-DNA relatedness data, indicated that strains R9SW1 T and A3d10 T represent two novel species of the genus Marinobacter. The names Marinobacter salarius sp. nov., with the type strain R9SW1 T ( = LMG 27497 T = JCM 19399 T = CIP 110588 T = KMM 7502 T ) and Marinobacter similis sp. nov., with the type strain A3d10 T ( = JCM 19398 T = CIP 110589 T = KMM 7501 T ), are proposed. Citation: Ng HJ, Ló pez-Pérez M, Webb HK, Gomez D, Sawabe T, et al. (2014) Marinobacter salarius sp. nov. and Marinobacter similis sp. nov., Isolated from Sea Water. PLoS ONE 9(9): e106514.
Marinobacterium aestuariivivens sp. nov., isolated from a tidal flat
International Journal of Systematic and Evolutionary Microbiology, 2016
A Gram-stain-negative, motile, aerobic, rod-or ovoid-shaped bacterium, designated DB-1 T , was isolated from a tidal flat on the Yellow Sea in South Korea and subjected to a taxonomic study using a polyphasic approach. Strain DB-1 T grew optimally at 30 8C, at pH 7.0-8.0 and in the presence of 0.5-2.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain DB-1 T falls within the clade comprising species of the genus Marinobacterium, clustering coherently with the type strain of Marinobacterium nitratireducens and showing a sequence similarity value of 98.4 %. The novel strain exhibited 16S rRNA gene sequence similarities of 91.5-94.4 % to the type strains of other species of the genus Marinobacterium. Strain DB-1 T contained Q-8 as the predominant ubiquinone and C 18 : 1 v7c, summed feature 3 (C 16 : 1 v7c and/or C 16 : 1 v6c) and C 16 : 0 as the major fatty acids. The major polar lipids detected in strain DB-1 T were phosphatidylethanolamine, phosphatidylglycerol, one unidentified aminolipid, one unidentified glycolipid, one unidentified phospholipid and two unidentified lipids. The DNA G+C content of strain DB-1 T was 62.3 mol% and the mean DNA-DNA relatedness value with the type strain of M. nitratireducens was 21¡4.6 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain DB-1 T is separated from recognized species of the genus Marinobacterium. On the basis of the data presented, strain DB-1 T is considered to represent a novel species of the genus Marinobacterium, for which the name Marinobacterium aestuariivivens sp. nov. is proposed. The type strain is DB-1 T (5KCTC 42778 T 5NBRC 111756 T).
Marinobacter guineae sp. nov., a novel moderately halophilic bacterium from an Antarctic environment
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008
Two Gram-negative, cold-adapted, moderately halophilic, aerobic bacteria, designated strains M3B T and M3T, were isolated from marine sediment collected from the South Shetland Islands, Antarctica. The organisms were rod-shaped, catalase-and oxidase-positive, and motile by means of polar flagella. These two psychrotolerant strains required Na + and grew at NaCl concentrations of 1-15 % and temperatures between 4 and 42 6C. 16S rRNA gene sequence analysis placed strains M3B T and M3T within the genus Marinobacter. DNA-DNA hybridization experiments between the Antarctic isolate M3B T and type strains of phylogenetically related species, namely Marinobacter lipolyticus, Marinobacter flavimaris, Marinobacter sediminum, Marinobacter algicola, Marinobacter maritimus and Marinobacter koreensis, revealed levels of relatedness lower than 32 %. Strain M3T showed 99 % DNA relatedness to strain M3B T . The DNA G+C contents of M3B T and M3T were 57.1 and 57.4 mol%, respectively, and their major isoprenoid quinone was ubiquinone-9. Several phenotypic characteristics, together with data on cellular fatty acid composition, served to differentiate strains M3B T and M3T from strains of related Marinobacter species. On the basis of the polyphasic taxonomic evidence presented in this study, it can be concluded that strains M3B T and M3T belong to the same genospecies and represent a novel species of the genus Marinobacter, for which the name Marinobacter guineae sp. nov. is proposed. The type strain is M3B T (5LMG 24048 T 5CECT 7243 T ).
Marinobacter goseongensis sp. nov., from seawater
International journal of systematic and evolutionary microbiology, 2008
A Gram-negative marine bacterium, designated strain En6 T , was isolated from seawater of the East Sea of Korea. The organism grew in 1-25 % (w/v) NaCl and at 10-37 6C and pH 5.3-9.3, with optimal growth occurring in 4-5 % NaCl and at 25-30 6C and pH 7.5. Phylogenetic analysis of the 16S rRNA gene sequence of strain En6 T placed this bacterium in the clade Marinobacter within the class Gammaproteobacteria. The 16S rRNA gene sequence similarity between strain En6 T and Marinobacter lipolyticus SM19 T , the most closely related species, was 98.4 %, and the level of DNA-DNA relatedness between the two strains was 22 %. On the basis of the phylogenetic analysis and phenotypic and chemotaxonomic data, strain En6 T is considered to represent a novel species of the genus Marinobacter. The name Marinobacter goseongensis sp. nov. is proposed, with strain En6 T (5KCTC 12515 T 5DSM 19471 T ) as the type strain.
Marinobacter excellens sp. nov., isolated from sediments of the Sea of Japan
International Journal of Systematic and Evolutionary Microbiology, 2003
Five strains of halophilic, Gram-negative marine bacteria (KMM 3809 T , KMM 3814, KMM 3815, KMM 3817 and KMM 3818) were isolated from sediments collected from Chazhma Bay, Sea of Japan. Phylogenetic 16S rRNA gene sequence-based analysis placed these bacteria in a clade within the genus Marinobacter in the c-Proteobacteria. KMM 3809 T showed highest 16S rRNA gene sequence similarity of 97?3 % to Marinobacter litoralis and 96?9 % to Marinobacter hydrocarbonoclasticus and Marinobacter aquaeolei. DNA-DNA hybridization between the five isolates was at the conspecific level (94-96 %) and that among the closest phylogenetic neighbours ranged from 45?0 to 62?5 %. The new organisms were susceptible to polymyxin. Predominant fatty acids were C 16 : 0 , C 16 : 1 v9c, C 16 : 1 v7c and C 18 : 1 v9c. Phylogenetic evidence, along with phenotypic and genotypic characteristics, showed that the bacteria constituted a novel species of the genus Marinobacter. The name Marinobacter excellens sp. nov. is proposed for this species, with the type strain KMM 3809 T (=CIP 107686 T ).
International Journal of Systematic and Evolutionary Microbiology, 2003
A Gram-negative, motile, non-spore-forming and moderately halophilic rod-shaped strain, SW-45 T , was isolated from sea water of the East Sea in Korea. The organism grew optimally at 30-37˚C and grew at 4 and 46˚C. It grew in the presence of 0?5-18 % (v/w) NaCl, with an optimum of 2-7 % NaCl. Strain SW-45 T was chemotaxonomically characterized by having ubiquinone-9 (Q-9) as the major respiratory lipoquinone and C 16 : 0 , C 18 : 1 v9c and C 16 : 1 v9c as the predominant fatty acids. The DNA G+C content was found to be 55 mol%. Phylogenetic analysis based on 16S rDNA sequences showed that strain SW-45 T forms a coherent cluster with the clade comprising the two Marinobacter species. 16S rDNA sequence similarities between strain SW-45 T and the Marinobacter species was 94?9 % to Marinobacter hydrocarbonoclasticus DSM 8798 T and 95?3 % to Marinobacter aquaeolei DSM 11845 T. Levels of DNA-DNA relatedness between strain SW-45 T and the type strains of M. hydrocarbonoclasticus and M. aquaeolei were respectively 4?3 and 5?5 %. On the basis of phenotypic properties, phylogeny and genomic data, strain SW-45 T (=KCCM 41591 T =JCM 11547 T) should be placed in the genus Marinobacter as a member of a novel species, for which the name Marinobacter litoralis sp. nov. is proposed. As part of this study, the major respiratory lipoquinone of M. hydrocarbonoclasticus and M. aquaeolei was also found to be Q-9. Abbreviations: FAME, fatty acid methyl ester; MA, marine agar 2216; MB, marine broth 2216. The GenBank accession number for the 16S rDNA sequence of strain SW-45 T is AF479689.
Marinobacterium halophilum sp. nov., a marine bacterium isolated from the Yellow Sea
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2007
A moderately halophilic, aerobic, Gram-negative bacterium was isolated from a tidal flat area of Dae-Chun, Chung-Nam, Korea. The strain, designated mano11 T , comprised rod-shaped cells that were motile by means of polar flagella. It grew with 3-12 % NaCl and at 4-37 6C and pH 5.3-9.3. The predominant menaquinone present in this strain was MK-7 and diaminopimelic acid was not found in the cell-wall peptidoglycan. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain mano11 T belongs to the genus Marinobacterium. Strain mano11 T exhibited 92.8-98.3 % 16S rRNA gene sequence similarity when compared with the type strains of three other species of the genus Marinobacterium. DNA-DNA hybridization between strain mano11 T and Marinobacterium georgiense DSM 11526 T , its closest relative in terms of 16S rRNA gene sequence similarity, was 13 %. On the basis of the phenotypic, genetic and phylogenetic data, strain mano11 T represents a novel species of the genus Marinobacterium, for which the name Marinobacterium halophilum sp. nov. is proposed. The type strain is mano11 T (=KCTC 12240 T =DSM 17586 T ).
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2006
Two aerobic, psychrohalotolerant, motile bacterial isolates, CK13 T and CK16, isolated from sea-water samples collected off the subantarctic Kerguelen island, were characterized by using a polyphasic taxonomic approach. On the basis of 16S rRNA gene sequence data, the strains were 99?6 % similar and exhibited 93-97 % similarity with the seven recognized species of Marinomonas. The most closely related species were Marinomonas pontica and Marinomonas primoryensis, with 97 and 96 % similarity at the 16S rRNA gene sequence level, respectively. DNA-DNA hybridization values between strain CK13 T and M. pontica and M. primoryensis were only 58 and 40 %, respectively. The major fatty acids present in strain CK13 T were iso-C 16 : 0 , C 16 : 0 , C 16 : 1 v7c and C 18 : 1 v7c. The DNA G+C content of strain CK13 T was 41?2 mol%. Phosphatidylethanolamine and phosphatidylglycerol were identified as the predominant phospholipids. All the above characteristics support the affiliation of strain CK13 T to the genus Marinomonas. Phylogenetic analysis and phenotypic and genotypic distinctiveness confirmed that strains CK13 T and CK16 are members of a novel species of the genus Marinomonas, for which the name Marinomonas polaris sp. nov. is proposed. The type strain is CK13 T (=MTCC 6645 T =DSM 16579 T =JCM 12522 T ).
Antonie van Leeuwenhoek, 2009
A novel Gram-negative, catalase-and oxidase-positive, non-sporulating, rod-shaped, aerobic bacterium, designated strain JSM 078120 T , was isolated from sea water collected from a tidal flat of Naozhou Island, South China Sea. Growth occurred with 1-15% (w/v) total salts (optimum, 2-4%), at pH 6.0-10.0 (optimum, pH 7.5) and at 4-35°C (optimum, 25-30°C). The major cellular fatty acids were C 18:1 x9c, C 16:0 , C 12:0 3-OH and C 16:1 x7c. The predominant respiratory quinone was ubiquinone Q-9, and the genomic DNA G ? C content was 60.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain JSM 078120 T should be assigned to the genus Marinobacter, being related most closely to the type strains of Marinobacter segnicrescens (sequence similarity 98.2%), Marinobacter bryozoorum (97.9%) and Marinobacter gudaonensis (97.6%). The sequence similarities between the novel isolate and the type strains of other recognized Marinobacter species ranged from 96.7 (with Marinobacter salsuginis) to 93.3% (with Marinobacter litoralis). The levels of DNA-DNA relatedness between strain JSM 078120 T and the type strains of M. segnicrescens, M. bryozoorum and M. gudaonensis were 25.3, 20.6 and 18.8%, respectively. The combination of phylogenetic analysis, DNA-DNA relatedness, phenotypic characteristics and chemotaxonomic data supported the view that strain JSM 078120 T represents a novel species of the genus Marinobacter, for which the name Marinobacter zhanjiangensis sp. nov. is proposed. The type strain is JSM 078120 T (= CCTCC AB 208029 T = DSM 21077 T = KCTC 22280 T). Keywords Marinobacter zhanjiangensis sp. nov. Á South China Sea The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain JSM 078120 T is FJ425903.
1999
Several strains of moderately halophilic and mesophil ic bacteria were isolated a t the head of an oil-producing well on an offshore platform in southern Vietnam. Cells were Gram-negative, non-spore-forming, rod-shaped and motile by means of a polar f lagellum. Growth occurred a t NaCl concentrations between 0 and 20%; the optimum was 5% NaCl. One strain, which was designated VT8l, could degrade n-hexadecane, pristane and some crude oil components. It grew anaerobically in the presence of nitrate on succinate, citrate or acetate, but not on glucose. Several organic acids and amino acids were utilized as sole carbon and energy sources. The major components of its cellular fatty acids were Clzr0 3-OH, c 1 6 : 1 09c, c16:o and C18:1 w9c. The DNA G+C content was 557 mol0/o. 165 rDNA sequence analysis indicated that strain VT8T was closely related to Marinobacter sp. strain CAB (99.8% similarity) and
Loading Preview
Sorry, preview is currently unavailable. You can download the paper by clicking the button above.