Marinobacter maritimus sp. nov., a psychrotolerant strain isolated from sea water off the subantarctic Kerguelen islands (original) (raw)

A psychrotolerant, Gram-negative, motile bacterium, designated CK 47 T , was isolated from sea water off the subantarctic Kerguelen islands (506 409 S 686 259 E). The isolate grew optimally at 22 6C and minimum and maximum temperature of growth were 4 and 37 6C, respectively. It required Na + for growth and exhibited optimum growth at pH 8?5 and 4 % NaCl. It utilized hexane, heptane and petroleum ether as sole sources of carbon. Strain CK 47 T had Q9 as the major respiratory quinone and C 16 : 0 (21?7 %), C 17 : 0 (21?3 %), C 18 : 0 (5?7 %), C 18 : 1 v7c (9?0 %) and C 18 : 1 v9c (31?4 %) as predominant fatty acids. The G+C content of the DNA was 58 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that CK 47 T formed a coherent cluster within the genus Marinobacter. It exhibited highest 16S rRNA gene sequence similarity of 96?8 % with Marinobacter lipolyticus. However, the level of DNA-DNA relatedness between strain CK47 T and M. lipolyticus was only 55 %. On the basis of phenotypic characteristics, and phylogenetic and genotypic distinctiveness, strain CK 47 T is considered to represent a novel species of the genus Marinobacter. The name Marinobacter maritimus sp. nov. is proposed, with CK 47 T (=JCM 12521 T =MTCC 6519 T ) as the type strain.