Retention of the differentiated state by larvalXenopus liver cells in primary culture (original) (raw)

Electron microscopic analysis of primary cultures derived from larval X e n o p u s liver has shown that these cells, although they form only two-dimensional aggregates, retain and presumably also develop structural characteristics typical of liver parenchyma cells, such as bile canaliculi with microvilli and epithelial junctional complexes. As judged from structural criteria, primary cultures contain 80 90% hepatocytes. In contrast to the intact tissue, primary cultures showed excessive development of microfilaments, however.