Staphylococcus aureus protein A induced inflammatory response in human corneal epithelial cells (original) (raw)
2007, Biochemical and Biophysical Research Communications
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Investigative ophthalmology & visual science, 2015
Purpose: To investigate the protective mechanisms evoked by TLR2 and MyD88 signaling in bacterial endophthalmitis in vivo. Methods: Endophthalmitis was induced in wild-type (WT), TLR2-/-, MyD88-/- , and Cnlp-/- mice by intravitreal injections of a laboratory (RN6390) and two endophthalmitis isolates of Staphylococcus aureus. Disease progression was monitored by assessing corneal and vitreous haze, bacterial burden, and retina tissue damage. The levels of inflammatory cytokines/chemokines were determined using qRT PCR and ELISA. Flowcytometry was used to assess neutrophil infiltration. CRAMP expression was determined by Immunostaining and dot blot. Results: Eyes infected with either laboratory or clinical isolates exhibited higher levels of inflammatory mediators at the early stages of infection (≤24h) in WT mice as compared to TLR2-/- or MyD88-/- mice. However, their levels surpassed that of WT mice at the later stages of infection (>48h), coinciding with increased bacterial burd...
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