Keratinocyte conditioned medium stimulates type IV collagenase synthesis in cultured human keratinocytes and fibroblasts (original) (raw)
1995, British Journal of Dermatology
We have previously shown that conditioned medium from cultured human keratinocytes stimulates proliferation of a variety of cell types involved in wound heaiing. as well as re-epithelialization of wounds in human skin in vitro. We now present evidence for an autocrine/paracrine control ofthe synthesis of type IV collagenases in human keratinocytes and tihrohlasts. During wound healing, keratinocytes migrate over the wound hed. an activity coupled with lysis of hasement membranes, and hence requiring the presence of collagenases. Collagenases are also needed for the production and remodelling of the granulation tissue. In order to study the autocrine/paracrine control of collagenase production in keratinocy:es and fibroblasts. we stimulated these cells in culture with conditioned medium from cultured keratinocytes. Protease synthesis was determined by affinity labelling with 'H-diisopropyllluorophosphoridate (DFP) and by zymography. Keratinocyte-conditioned medium was found to increase the expression of 72 and 92 kDa type IV coliagenase in human keratinocytes, and the 72 kDa collagenase in human tibroblasts. indicating that an autocrine/ paracrine control mechanism is invoked in collagenase production in these cell types during wound healing. This increased expression of collagenases could he partly responsible for the stimulated healing seen in wounds treated with sheets of cultured keratinocytes.
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