Enhanced albumin affinity of silicone rubber (original) (raw)

ASAIO transactions / American Society for Artificial Internal Organs

Abstract

The authors have developed a method to increase the albumin affinity of silicone rubber-containing medical devices. A vinyl-methyl silicone co-monomer was hydroxylated via an oxymercuration-demercuration reaction and then film-coated on a silicone rubber sheet. C16 acylation of the -OH coated sheet was carried out by an esterification reaction catalyzed by 4-DMAP. FTIR spectroscopy confirmed the presence of -OH groups following the hydroxylation reaction, and (CH2)n groups following the acylation reaction. 125I-labeled albumin binding studies indicated graded enhancements, according to reagent concentration, with maximal 2.7X and 2.5X increases for 20% -OH coated and 20% -OH/C16 acylated surfaces, respectively. Incubation with a protein denaturant, sodium dodecyl sulfate (SDS), removed 73% of adsorbed albumin from unmodified controls, with only 25 and 34% removal from 20% -OH coated and 20% -OH/C16 acylated surfaces, respectively. These results suggest that -OH and C16 modified surf...

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