Presence and distribution of serotonin immunoreactivity in the cyprids of the barnacle Balanus amphitrite (original) (raw)
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Microscopy Research and Technique, 2009
The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barnacle Balanus amphitrite were investigated using immunohistochemical methods. Barnacles are considered to be one of the most important constituents of animal fouling communities, and the cyprid stage is specialized for settlement and metamorphosis in to the sessile adult condition. FLPs immunoreactive (IR) neuronal cell bodies were detected in both the central and the peripheral nervous system. One bilateral group of neurons somata was immunodetected in the brain, and IR nerve fibers were observed in the neuropil area and optic lobes. Intense immunostaining was also observed in the frontal filament complex: frontal filament tracts leaving the optic lobes and projecting towards the compound eyes, swollen nerve endings in the frontal filament vesicles, and thin nerve endings in the external frontal filament. Thin IR nerve fibers were also present in the cement glands. Two pairs of neuronal cell bodies were immunodetected in the posterior ganglion; some of their axons appear to project to the cirri. FLPs IR neuronal cell bodies were also localized in the wall of the dilated midgut and in the narrow hindgut; their processes surround the gut wall and allow gut neurons to synapse with one another. Our data demonstrated the presence of FLPs IR substances in the barnacle cyprid. We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role. Microsc. Res. Tech., 2009. © 2008 Wiley-Liss, Inc.
Distribution and characterization of neuropeptide Y in the brain of an elasmobranch fish
Brain Research, 1988
Monoclonal antibodies against neuropeptide FF were produced and characterized. The antibodies are directed and highly specific to neuropeptide FF, and reactivity requires the C-terminal dipeptide of neuropeptide FF (Arg-Phe-NH,). Tissue extracts from bovine spinal cord, rat spinal cord and hypothalamus were analysed by high-pressure liquid chromatography coupled with radioimmunoassay using the characterized monoclonal antibody. Only one immunoreactive peptide was detected and it coeluted with authentic neuropeptide FF. Using this highly specific monoclonal antibody, the distribution of neuropeptide FF-like immunoreactivity was further studied by indirect immunohistochemistry. lmmunoreactivity was seen in two major cell groups in the rat brain. The largest cell group was located in the medial hypothalamus between the dorsomedial and ventromedial nuclei. The other one was found in the nucleus of the solitary tract. Fibres immunoreactive for neuropeptide FF were located in the lateral septa1 nucleus, amygdala, different hypothalamic areas, nucleus of the solitary tract, ventral medulla, trigeminal complex and the dorsal horn of the spinal cord. Spinal and sympathetic ganglia were non-reactive. No neuropeptide FF irnmunoreactivity was seen in the gut autonomic nervous system or endocrine cells. The results show that neuropeptide FF-like immunoreactivity has a clearly more limited distribution in the nervous system than typical brain-gut peptides.
Cell & Tissue Research, 1995
The distribution and morphology of crustacean cardioactive peptide-immunoreactive neurons in the brain of the locust Locusta migratoria has been determined. Of more than 500 immunoreactive neurons in total, about 380 are interneurons in the optic lobes. These neurons invade several layers of the medulla and distal parts of the lobula. In addition, a small group of neurons projects into the accessory medulla, the lamina, and to several areas in the median protocerebrum. In the midbrain, 12 groups or individual neurons have been reconstructed. Four groups innervate areas of the superior lateral and ventral lateral protocerebrum and the lateral horn. Two cell groups have bilateral arborizations anterior and posterior to the central body or in the superior median protocerebrum. Ramifications in subunits of the central body and in the lateral and the median accessory lobes arise from four additional cell groups. Two local interneurons innervate the antennal lobe. A tritocerebral cell projects contralaterally into the frontal ganglion and appears to give rise to fibers in the recurrent nerve, and in the hypocerebral and ingluvial ganglia. Varicose fibers in the nervi corporis cardiaci III and the corpora cardiaca, and terminals on pharyngeal dilator muscles arise from two subesophageal neurons. Some of the locust neurons closely resemble immunopositive neurons in a beetle and a moth. Our results suggest that the peptide may be (1) a modulatory substance produced by many brain interneurons, and (2) a neurohormone released from subesophageal neurosecretory cells.
Histochemistry, 1988
The presence and distribution of bombesin-, enkephalin-, gastrin/cholecystokinin-, neuropeptide Y-, neurotensin-, somatostatin-, substance P-, and VIP-like immunoreactivities in gut nerves of representatives of nineteen cyclostome, elasmobranch and teleost species have been studied.
Immunohistochemical staining revealed proctolin-like immunoreactivity in nerve endings associated with the hindgut of the crayfish Procambarus clarkii. Proctolin-like bioactivity, detected using both locust oviducts and crayfish hindguts for bioassays, co-eluted with authentic proctolin through five consecutive reversed-phase high-performance liquid chromatography (RP-HPLC) systems. This strongly suggests that proctolin (or a peptide very similar to it) is contained in nerve endings on the crayfish hindgut. RFamide-like immunoreactivity (RFLI) was extracted from the hindguts and intestinal nerves of crayfish and separated using RP-HPLC. Initial separation on a C18 column gave a broad peak of RFLI, and these fractions were bioactive on the crayfish hindgut. Subsequently, RFLI was separated on two additional RP-HPLC systems. The predominant peak could be distinguished from FMRFamide and several known FMRFamide-like peptides on the basis of elution times. Partial sequence analysis indicated the presence of a decapeptide having some sequence homology with leucomyosuppressin and SchistoFLRFamide. These results support earlier evidence that extended RFamide peptides may function as neurotransmitters or neuromodulators on the crayfish hindgut, and suggest a similar role for proctolin or a closely related peptide.
Annals of the New York Academy of Sciences, 1990
During the last few years an increasing number of investigations into insect neurohormones have been performed and it now appears that neuropeptides are involved in most insect regulatory processes. Several insect neuropeptides such as bombyxin, locustasulfakinin and leucosulfakinin, the locust diuretic hormone and the locustatachykinins exhibit sequence homologies with vertebrate hormones, insulin, gastridcholecystokinin, arginine-vasotocin and tachykinins respectively. ' We have demonstrated the presence of substances related to the vertebrate 36 amino acid peptide NPY (neuropeptide tyrosine) in two insect species, Locusta migratoria and Sarcophaga bullata. NPY has a widespread distribution and is present in extremely high concentrations in the brain of vertebrates. The distribution of NPY-like substances in the nervous system and the midgut of the migratory locust, and in the brain of the fleshfly was determined by immunocytochemistry using an antiserum directed against synthetic porcine NPY. By means of the peroxidase-antiperoxidase method, NPY-immunoreactive perikarya were detected in the brain , optic lobes, corpora cardiaca, suboesophageal ganglion and ventral nerve cord of the locust and in the brain, optic lobes and suboesophageal ganglion of the fleshfly.