Analysis of associative learning in the terrestrial mollusc Limax maximus. II. Appetitive learning (original) (raw)
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An analysis of associative learning in a terrestrial mollusc
Journal of Comparative Physiology ? A, 1981
We have recently demonstrated that associative learning can play an important role in the regulation of food selection behavior of Limax maxirnus, a terrestrial mollusc. The tendency of Limax to approach a normally attractive odor generated by a food source such as carrot or potato can be markedly reduced if exposure to that odor is paired with exposure to a bitter taste (quinidine sulfate). We now report that variables known to influence associative learning by vertebrates (the operations of a secondorder conditioning procedure, blocking and US-preexposure) similarly influence associative learning by Limax.
One-trial associative learning modifies food odor preferences of a terrestrial mollusc
Proceedings of the National Academy of Sciences, 1981
We present evidence of rapid and reliable associative learning by the terrestrial mollusc, Limax maximus. Slugs were exposed once to a pairing ofa highly attractive food odor (potato or carrot) and a saturated solution of quinidine sulfate, a bitter-tasting plant substance. In comparison with control slugs, the exposed slugs subsequently displayed a markedly reduced preference for the odor paired with quinidine. This reduced odor preference was limited to the specific odor paired with quinidine and did not generalize to other food odors.
Physiology & …, 1997
Elemental and configural learning and the perception of odorant mixtures by the spiny lobster Panulirus argus. PHYSIOL BEHAV 62 (1) 169-174, 1997.-The present study used a conditioning assay to investigate if the type of learning task that spiny lobsters (Panulirus argus) were required to perform influenced the way that they perceived odorant mixtures. Mixtures were composed of 2 food-related compounds (adenosine-5-monophosphate, betaine, or L-glutamate) at concentrations that produced the same duration of searching behavior in unconditioned animals. Aversive conditioning of search behavior coupled with generalization testing was used to evaluate perceptual similarity between related mixtures. When animals were conditioned to stop searching to a binary mixture AX, they did not generalize significantly from this mixture to either of its components (A or X) , or to a binary mixture containing one novel component (AY). However, when lobsters were conditioned to avoid AX but to continue responding to AY, they generalized between AX and X and between AY and Y. The results support the hypothesis that altering the salience of a mixture's components by giving them different reinforcement contingencies changed the way that the mixtures were perceived. As a result of such conditioning, animals perceived the mixture's components as separate elements, rather than as a configuration, and, as a consequence, animals generalized between binary mixtures and their most salient or predictive components.
Learning & Memory, 1998
We compared behaviorally and physiologically the olfactory responses of slugs (Limax marginatus) that had been subjected to aversive, appetitive, or unpaired training with food odors (carrot or cucumber). In the aversive training, the slugs were exposed to the food odor as a conditioned stimulus (CS), and then quinidine sulfate solution as an unconditioned stimulus (UCS) was immediately applied to the lip of the slugs. This training caused a decrease in preference level for the CS. The unpaired training, in which the CS and the UCS were presented to the slugs with a 5-min interval, induced no change in the preference level for the CS. In the appetitive training, the slugs were allowed to eat the CS odor source without UCS application. When we used nonstarved slugs, it was found that the preference level for the CS increased upon the appetitive training. These results indicate that each training changed the preference for the odors in a characteristic manner. In the physiological exp...
Pavlovian appetitive discriminative conditioning inAplysia californica
Learning & behavior, 1997
Three experiments demonstrated Pavlovian appetitive discrimination learning in the marine mollusc, Aplysia californica. In each experiment, subjects were exposed to two conditioned stimuli; one stimulus (CS+) was paired with food presentations and the other stimulus (CS-) was never followed by food. In Experiments 1 and 3 different chemosensory stimuli were used, and in Experiment 2 different tactile stimuli were used. For both types of conditioned stimuli, bite responses occurred significantly more often to the CS+ than to the CS-. Experiment 2 also showed that Aplysia could learn a reversal of this discrimination. Experiment 3 showed that nonreinforced presentations of CS+ resulted in a decline in the frequency of conditioned biting. The implications of these results for neurobiological analyses of learning are discussed.
Taste discrimination in conditioned taste aversion of the pond snail Lymnaea stagnalis
Journal of Experimental Biology, 2006
Lymnaea stagnalis has been widely used as a model for gaining an understanding of the molecular and behavioral mechanisms underlying learning and memory. At the behavioral level, however, it is still unclear how taste discrimination and CTA interact. We thus examined how CTA to one taste affected the feeding response induced by another appetitive food stimulus. We first demonstrated that snails have the capacity to recognize sucrose and carrot juice as distinct appetitive stimuli. We then found that snails can become conditioned (i.e. CTA) to avoid one of the stimuli and not the other. These results show that snails can distinguish between appetitive stimuli during CTA, suggesting that taste discrimination is processed upstream of the site where memory consolidation in the snail brain occurs. Moreover, we examined second-order conditioning with two appetitive stimuli and one aversive stimulus. Snails acquired second-order conditioning and were still able to distinguish between the different stimuli. Finally, we repeatedly presented the conditional stimulus alone to the conditioned snails, but this procedure did not extinguish the long-term memory of CTA in the snails. Taken together, our data suggest that CTA causes specific, irreversible and rigid changes from appetitive stimuli to aversive ones in the conditioning procedure.
Appetitive and Aversive Learning in Spodoptera littoralis Larvae
Chemical Senses, 2011
Adult Lepidoptera are capable of associative learning. This helps them to forage flowers or to find suitable oviposition sites. Larval learning has never been seriously considered because they have limited foraging capabilities and usually depend on adults as concerns their food choices. We tested if Spodoptera littoralis larvae can learn to associate an odor with a tastant using a new classical conditioning paradigm. Groups of larvae were exposed to an unconditioned stimulus (US: fructose or quinine mixed with agar) paired with a conditioned stimulus (CS: hexanol, geraniol or pentyl acetate) in a petri dish. Their reaction to CS was subsequently tested in a petri dish at different time intervals after conditioning. Trained larvae showed a significant preference or avoidance to CS when paired with US depending on the reinforcer used. The training was more efficient when larvae were given a choice between an area where CS-US was paired and an area with no CS (or another odor). In these conditions, the memory formed could be recalled at least 24 h after pairing with an aversive stimulus and only 5 min after pairing with an appetitive stimulus. This learning was specific to CS because trained larvae were able to discriminate CS from another odor that was present during the training but unrewarded. These results suggest that Lepidoptera larvae exhibit more behavioral plasticity than previously appreciated.
Associative learning between odorants and mechanosensory punishment in larval Drosophila
Journal of Experimental Biology, 2011
We tested whether Drosophila larvae can associate odours with a mechanosensory disturbance as a punishment, using substrate vibration conveyed by a loudspeaker (buzz: ). One odour (A) was presented with the buzz, while another odour (B) was presented without the buzz (A/B training). Then, animals were offered the choice between A and B. After reciprocal training (A/B), a second experimental group was tested in the same way. We found that larvae show conditioned escape from the previously punished odour. We further report an increase of associative performance scores with the number of punishments, and an increase according to the number of training cycles. Within the range tested (between 50 and 200Hz), however, the pitch of the buzz does not apparently impact associative success. Last, but not least, we characterized odour-buzz memories with regard to the conditions under which they are behaviourally expressed -or not. In accordance with what has previously been found for associative learning between odours and bad taste (such as high concentration salt or quinine), we report that conditioned escape after odour-buzz learning is disabled if escape is not warranted, i.e. if no punishment to escape from is present during testing. Together with the already established paradigms for the association of odour and bad taste, the present assay offers the prospect of analysing how a relatively simple brain orchestrates memory and behaviour with regard to different kinds of ʻbadʼ events.