Effect of Creatine on Rat Inner Ear Stem Cell Culture (original) (raw)

Otolaryngology -- Head and Neck Surgery, 2012

Abstract

Objective: Investigate whether creatine might enhance the long lasting propagation and differentiation of inner ear stem cells. Method: Rat inner ear stem cells were propagated in basal medium. Creatine supplementation was at third passage. Self-renewal capacity initiated measured by the spheres number in each passage. Cultures was grown for 2 weeks in creatine absence or presence, and neuronal and hair cell numbers were analyzed by beta-III- tubulin and myosin VIIa immunohistochemistry, respectively. Results: Stem cells were propagated for 11 passages for utricle and organ of Corti and 6 for spiral ganglion derived spheres. Creatine significantly enhanced the number of propagated spheres from P3 onward. Creatine promoted the spiral ganglia- derived spheres number and significantly increased the yield of neuronal cells of differentiated progenitors by 40%. It had no effect on differentiation in cultures derived from utricle and organ of Corti. Conclusion: Creatine supplementation promoted both the propagation of rat spiral ganglia, utricle, and organ of Corti, as well as neural differentiation of spiral ganglia stem cells.

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