Green Synthesis of Silver Nanoparticles using Psidium Guajava Leaf Extract and their Characterization (original) (raw)
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Aqueous Stable Silver Nanoparticles Derived from Nimble Leaves of Psidium Guajava
Integrative Journal of Conference Proceedings
Here, we report the synthesis of silver nanoparticles (AgNPs) by utilizing extract of Psidium guajava. In this study leaf was with extricated alcohol, further this extract is utilized for the preparation of nanoparticles followed by treatment with silver nitrate solution. Color change from colorless to dark-yellow and SPR band (UV-Vis) 420nm which indicates the synthesis of plant reduced silver nanoparticles. The presences of proteins as capping agent, which increase the stability of AgNPs in the colloids, are characterized by Fourier transform infrared spectroscopy (FTIR). Transmission electron microscopy (TEM) demonstrated the presence at spherical AgNPs in the range of 15-30nm. The bactericidal activity of standard antibiotics was essentially expanded in the presence of AgNPs against pathogenic bacteria, S Aureus, Staphylococcus, E Coli, P Putida and fungus A Niger.
Article, 2022
In this Research work, Silver Nanoparticles were green synthesized from Psidium guajava leaves and different Characterization techniques including UV-Visible, FT-IR, SEM and XRD were all employed to ascertain the absorption peaks, functional group, surface morphology and crystalline size of the nanoparticles respectively. These nanoparticles green synthesized were applied against four different pathogens namely, S. aureus (gram-positive bacteria), E. coli (gram-negative bacteria), C. albicans (gram-positive fungus) and Aspergillus niger and the investigation showed that the Silver nanoparticles synthesized were potent against the selected pathogens. From the UV-Vis spectral analysis, it was observed that highest absorption peaks appeared at 400nm and 500nm reflecting the surface plasmon resonance of silver Nanoparticles from guava leaves which is characteristic of Silver Nanoparticles. From the FT-IR spectrum of the sample under studied, the peaks 3416.85 cm-1 , 2923.51 cm-1 , 1618.95 cm-1 , 1384.49 cm-1 and 1033.63 cm-1 were observed where the absorption band at 3416.85 cm-1 corresponds to the stretching due to N-H, while the band at 2923.51 cm-1 is associated with C-H stretch of alkane and O-H stretching. The peak at 1618.95 cm-1 shows C=C stretching, 1384.49 cm-1 reveals the existence of C-H bending and 1033.63 cm-1 depicted CO stretching. The SEM analysis revealed the shape of the nanoparticles as being spherical while XRD result admits that the average size of the green synthesized Ag NPs was 45.5 nm using the Scherer's formula. Augmentin was used as control at concentration of 300μg/L throughout antimicrobial studies. Different concentrations of 100, 200, 300, 400 and 500μg/L of Silver Nanoparticles were tested against each pathogen. It was discovered that with increase in concentrations of Silver Nanoparticles of all the pathogens, there generally appeared to be increase in inhibition zone. At higher concentration of 500μg/L, the zones of inhibition were in the following order; 22.50 mm, 17.00mm, 15.44mm, and 13.23mm for E. Coli, S. aureus, C. albicans and Aspergillus niger respectively. For each concentration investigated, E. coli, demonstrated higher zone of inhibition as opposed to all other pathogens investigated in this research.
Online journal of chemistry, 2022
In this Research work, Silver Nanoparticles were green synthesized from Psidium guajava leaves and different Characterization techniques including UV-Visible, FT-IR, SEM and XRD were all employed to ascertain the absorption peaks, functional group, surface morphology and crystalline size of the nanoparticles respectively. These nanoparticles green synthesized were applied against four different pathogens namely, S. aureus (gram-positive bacteria), E. coli (gram-negative bacteria), C. albicans (gram-positive fungus) and Aspergillus niger and the investigation showed that the Silver nanoparticles synthesized were potent against the selected pathogens. From the UV-Vis spectral analysis, it was observed that highest absorption peaks appeared at 400nm and 500nm reflecting the surface plasmon resonance of silver Nanoparticles from guava leaves which is characteristic of Silver Nanoparticles. From the FT-IR spectrum of the sample under studied, the peaks 3416.85 cm-1 , 2923.51 cm-1 , 1618.95 cm-1 , 1384.49 cm-1 and 1033.63 cm-1 were observed where the absorption band at 3416.85 cm-1 corresponds to the stretching due to N-H, while the band at 2923.51 cm-1 is associated with C-H stretch of alkane and O-H stretching. The peak at 1618.95 cm-1 shows C=C stretching, 1384.49 cm-1 reveals the existence of C-H bending and 1033.63 cm-1 depicted CO stretching. The SEM analysis revealed the shape of the nanoparticles as being spherical while XRD result admits that the average size of the green synthesized Ag NPs was 45.5 nm using the Scherer's formula. Augmentin was used as control at concentration of 300μg/L throughout antimicrobial studies. Different concentrations of 100, 200, 300, 400 and 500μg/L of Silver Nanoparticles were tested against each pathogen. It was discovered that with increase in concentrations of Silver Nanoparticles of all the pathogens, there generally appeared to be increase in inhibition zone. At higher concentration of 500μg/L, the zones of inhibition were in the following order; 22.50 mm, 17.00mm, 15.44mm, and 13.23mm for E. Coli, S. aureus, C. albicans and Aspergillus niger respectively. For each concentration investigated, E. coli, demonstrated higher zone of inhibition as opposed to all other pathogens investigated in this research.
Green Synthesis of Silver Nanoparticles by Papaya Peel Extract
2021
This study was to investigate an efficient, cost effective and sustainable route of silver nanoparticles (AgNP) from silver nitrate. In this natural green synthesis, papaya peel extract which act as natural reducing agent to synthesize silver nanoparticles. The characteristic color is changed to reddish brown in the reaction due to reduction. The AgNPs were characterized by UV-visible (vis) spectrophotometer, FTIR and scanning electron microscopy (SEM) with EDAX. The synthesized silver nanoparticles (AgNPs) were visually confirmed with surface plasmon resonance peak at 440 nm upon UV–Vis spectroscopy analysis. Fourier transform infrared spectroscopy (FT-IR) affirmed the role of fruit extract as a reducing and capping agent of silver ions. Furthermore, the nanoparticles formation were characterized with different pH, temperature and stability of the nanoparticles. AgNPs displayed strong antibacterial potentials against Gram-positive and Gram-negative bacteria. Papaya peel -AgNPs also...
INVESTIGATION OF BIOGENIC SILVER NANOPARTICLES GREEN SYNTHESIZED FROM CARICA PAPAYA
This work mainly focuses on the investigation of optical, structural and morphological characteristics of Silver nanoparticles (Ag-NPs) synthesized using Papaya (Carica Papaya) leaf extract and to study the mechanisms involved in the formation.Papaya is a good reducing agent for the synthesis of Ag-NPs. Ag-NPs were synthesized using colloidal method from silver nitrate using aqueous leaf extract of Papaya as reducing agent. Spectral analysis of Ag-NPs was done using UV-Vis spectroscopy and optical characteristics were studied. Fourier Transform Infrared (FTIR) analysis was done to investigate the mechanisms involved in the reaction. A detailed study of structural and morphological properties was done using X-Ray Diffraction (XRD) and Atomic Force Microscopy (AFM).Spherical shaped Ag-NPs of Face Centered Cubic (FCC) structure were formed and the average particle size was in the range 25-35 nm. Since it is a plant with medicinal values, thus synthesized Ag-NPs can be used for medicinal implications.
Interest in the biosynthesis of nanoparticles has increased in the last era by researchers. Nanoparticles have several applications in different fields like optoelectronics, magnetic devices, drug delivery, and sensors. Nanoparticle synthesis by green methods is safe for the environment and should be explored and encouraged popularly since various plants' have the high extent to form these nanoparticles. Worldwide, UV spectroscopy, Xray diffraction, Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) besides Fourier Transform Infrared Spectroscopy (FTIR) are used in many ways for characterize nanoparticles. The most advantageous use of AgNPs is their great attribution to be used as antimicrobial agents. Finally, concept of AgNPs synthesis is deserved to be the modern technical and medical concern. The current review shows a complete comprehensive and analytical survey of the biosynthesis of AgNPs with a particular focus on their activities as antimicrobials and the possible theories of their effect on the microbial cell and all influenced secondary metabolites.
Green Synthesis of Silver Nanoparticles Using Alagaw Leaf Extract
Green Synthesis of Silver Nanoparticles Using Alagaw (Premna odorata) Leaf Extract, 2019
Abstract. There is a worldwide interest in silver nanoparticles (AgNPs) synthesize by various chemical reactions for use in applications. Silver nanoparticles have gained significant interest due to their unique optical, antimicrobial, electrical, physical properties and their possible application. However, it is necessary to develop environmental friendly methods for their syntheses. To avoid chemically toxicity, biosynthesis of metal silver nanoparticles is proposed as a cost-effective and environmental friendly alternative. This study aimed to find out whether Alagaw plant can potentially act as a reducing agent for the biosynthesis of silver nanoparticles and whether the concentration of the leaf extract can affect the absorbance spectrum, size and shape of the synthesized silver nanoparticles. The synthesized silver nanoparticles were characterized using the UV-vis spectroscopy for its absorbance spectrum and Transmission Electron Microscope Analysis for its morphology and size. The experimental method of research was used using three treatments and replicates of the different concentrations of Alagaw leaf extract: Treatment A (0.2 g/mL), Treatment B (0.4 g/mL) and Treatment C (0.6 g/mL) with 10 minutes and 60 minutes interval of observation under UV-vis spectrophotometer. Based on the findings of the study, Alagaw plant can potentially act as a good reducing agent for the biosynthesis of silver nanoparticles. The results recorded from UV-vis spectrophotometer support the biosynthesis and characterization of silver nanoparticles that as the concentration of the leaf extract increases it significantly affect the wavelength peaks and absorbance peaks of the synthesized silver nanoparticles. Using the high-resolution Transmission Electron Microscopy, the size of silver nanoparticles measured 50 nm – 100 nm having near-spherical in shape. Keywords: Nanoparticles, Biosynthesis, Spectroscopy, Absorbance Spectrum
INVESTIGATION OF BIOGENIC SILVER NANOPARTICLES GREEN SYNTHESIZED FROM CARICA PAPAYA Original Article
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: This work mainly focuses on the investigation of optical, structural and morphological characteristics of Silver nanoparticles (Ag-NPs) synthesized using Papaya (Carica Papaya) leaf extract and to study the mechanisms involved in the formation. Methods: Ag-NPs were synthesized using colloidal method from silver nitrate using aqueous leaf extract of Papaya as reducing agent. Spectral analysis of Ag-NPs was done using UV-Vis spectroscopy and optical characteristics were studied. Fourier Transform Infrared (FTIR) analysis was done to investigate the mechanisms involved in the reaction. A detailed study of structural and morphological properties was done using X-Ray Diffraction (XRD) and Atomic Force Microscopy (AFM). Results: Spherical shaped Ag-NPs of Face Centered Cubic (FCC) structure were formed and the average particle size was in the range 25-35 nm. Conclusion: Papaya is a good reducing agent for the synthesis of Ag-NPs. Since it is a plant with medicinal values, thus synthesized Ag-NPs can be used for medicinal implications.
Antibacterial and Cytotoxic Potential of Biosynthesized Silver Nanoparticles by Some Plant Extracts
Nanomaterials
The provision of nanoparticles using biogenic material as a part of green chemistry is an attractive nanotechnology. The current research aimed to test the antimicrobial and cytotoxic efficacy of silver nanoparticles synthesized by extracts of Phoenix dactylifera, Ferula asafetida, and Acacia nilotica as reductant and stabilizing agents in silver nanoparticle formation. Synthesized nanoparticles were evaluated for their antimicrobial activity against Staphylococcus aureus (Gram-positive) and Pseudomonas aeruginosa and Escherichia coli (Gram-negative) using an agar well diffusion assay. Furthermore, cytotoxic ability was investigated against LoVo cells. The potential phyto-constituents of plant extracts were identified by Fourier-transform infrared spectroscopy (FT-IR) techniques. Field emission scanning electron microscopy (FE-SEM), transmission electron microscope (TEM), and zeta potential analyzed the size and morphology of the biogenic nanoparticles. The current study revealed the ability of the tested plant extract to convert silver ions to silver nanoparticles with an average size that ranged between 67.8 ± 0.3 and 155.7 ± 1.5 nm in diameter. Biogenic AgNPs showed significant antibacterial ability (10 to 32 mm diameter) and anticancer ability against a LoVo cell with IC50 ranged between 35.15-56.73 µg/mL. The innovation of the present study is that the green synthesis of NPs, which is simple and cost effective, provides stable nano-materials and can be an alternative for the large-scale synthesis of silver nanoparticles.