Corresponding Detection of human cytomegalovirus by using PCR in immunocompromised persons (original) (raw)
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─ BACKGROUND AND OBJECTIVES: Human cytomegalovirus (HCMV) is a member of the family herpes viruses and early recognition of the importance of this virus is quite a lot of people are having is immunodeficiency, because of this, reason the extent of clinical protests infection to this virus in these patients, due reduced levels of their immune, the aim of this study was to detect the CMV virus quickly and check the accuracy of the two methods of Real-time PCR and cytochemical in immunocompromised people for HCMV defects. METHODS: 100 blood samples were collected from immunocompromised persons in Tehran. Real-time PCR and cytochemical staining two methods for the detection of HCMV was used. The first method is based on the Taq Man and second method was based is based on the characterization of the cell morphological identification. RESULTS: Those who PCR test results was positive, 40werecase (36%). Whereas, this number in of Cytochemical a total of 35 were cases (21 percent).It was also found that the maximum prevalence in the summer season (34%) and minimum its abundance belong to the autumn season (22%) is. However, a significant relationship was no observed between the prevalence of HCMV and the season. CONCLUSION: Results of this study showed that with greater accuracy Real-time PCR able to recognize HCMV infection in individuals is the immunocompromised. but cytochemical stains method can be used along with this method for the final confirmation and very increase the accuracy of diagnosis, Was also found that the highest rate of HCMV infection either latent or active form, is the related to the fall season, but this association were not significant.
HCMV research modifiedبعد التعديل
د. آالء زنزل رعد الدوري فرع األحياء المجهرية / كلية الطب / جامعة تكريت / العراق 2 Estimation of some immunological biomarkers in aborted women infected with human cytomegalovirus (HCMV) in Salah Al-deen province Abstract:
Journal of clinical microbiology, 2000
In the present prospective study, five blood tests for detection of human cytomegalovirus (HCMV), nucleic acid sequence-based amplification (NASBA) for detection of early (immediate-early antigen) and late (pp67) mRNA, PCR for detection of HCMV DNA (DNA PCR), culture, and pp65 antigenemia assay, and culture and DNA PCR of urine and throat swab specimens were compared for their abilities to predict the development of disease caused by HCMV (HCMV disease). Of 101 human immunodeficiency virus (HIV)-infected patients with </=100 CD4(+) lymphocytes per mm(3), 25 patients developed HCMV disease. The pp65 antigenemia assay (sensitivity, 50%; specificity, 89%) and DNA PCR of blood (sensitivity, 69%; specificity, 75%) were most accurate in predicting the development of HCMV disease within the next 12 months. Both blood culture and late pp67 mRNA NASBA had high specificities (91 and 90%, respectively) but low sensitivities (25 and 13%, respectively). The sensitivities of urine culture, DNA...
Assessment of human cytomegalovirus co-infection in Egyptian chronic HCV patients
Virology Journal, 2011
Human cytomegalovirus (HCMV) is the most common cause of severe morbidity and mortality in immune- compromised individuals. This study was conducted to determine the incidence of HCMV infection in HCV patients who either spontaneously cleared the virus or progressed to chronic HCV infection. The study included a total of eighty four cases (48 females and 36 males) that were referred to blood banks for blood donation with an age range of 18-64 years (mean age 37.62 ± 10.03 years). Hepatitis C virus RNA and HCMV DNA were detected in sera by RT-nested PCR and nested PCR respectively in all subjects. Immunoglobulin G levels for HCV and HCMV were determined. Besides, IgM antibodies for HCMV infection were also determined in subjects' sera. Fifty three out of 84 cases (63%) were positive for HCV-RNA while 31 (37%) cases had negative HCV RNA. Forty six (87%) and 13 (25%) cases out of 53 HCV RNA positive patients were positive for HCMV IgG and IgM antibodies respectively. While 20 of 53 cases (38%) had detectable HCMV DNA. To examine the role of HCMV infection in HCV spontaneous resolution, two groups of HCV patients, group 1) chronic HCV infection (positive HCV RNA and positive IgG antibodies) vs group 2) spontaneous resolution (negative HCV RNA and positive IgG antibodies) were compared. The percentages of positive CMV IgG and IgM results is higher in chronic HCV patient than those in spontaneously cleared HCV patients and the difference is highly statistically significant (P value < 0.001). Also, there is a general trend towards elevated levels of CMV IgG antibodies in HCV chronic patients than those in spontaneously cleared HCV patients (P value < 0.02). HCMV DNA detection in group 1 was more than twice the value observed in group 2 (38% vs 14.3%, P value < 0.001). Moreover, levels of liver enzymes were significantly higher in HCV RNA positive cases co-infected with HCMV DNA than HCMV negative cases (P value < 0.001). The results indicate the role of HCMV in the liver pathogenesis. We conclude that chronic HCV patients co-infected with HCMV infection can be regarded as high risk groups for liver disease progression where they should be monitored for the long term outcome of the disease.
Journal of Virological Methods, 2014
In order to determine the effect of the increase in sensitivity of HCMV detection in whole blood compared to plasma on reproductive rate (R o) measurement, an optimized human cytomegalovirus (HCMV) quantitative PCR assay was developed. The results presented in this study are summarized by the following three methodological improvements: (i) at values below the limit of quantitation (LOQ) of 60 copies/ml, determination of HCMV load was more sensitive with whole blood than plasma, (ii) for the determination of viral load, whole blood was more sensitive than plasma below 1000 copies/ml but little difference was observed above 1000 copies/ml and (iii) the measurement of "Reproductive Rate" can be affected by imprecise measurement of HCMV viral load in either plasma or whole blood compartments depending on whether samples were taken from patients on antiviral treatment or from patients where HCMV load was rising. Taken together this study provides methodological improvements suggesting that below HCMV viral load levels of 1000 copies/ml (1640 IU/ml) both plasma and whole blood should be tested.
PCR activity of CMV in healthy CMV-seropositive individuals: does latency need redefinition?
Research in Virology, 1996
To demonstrate a possible association between stress factors and the presence of human cytomegalovirus (HCMV) DNA in leukocytes and in cell-free body fluids, at 2-week intervals over a bmonth period, specimens were taken for HCMV DNA testing from 11 healthy CMV-seropositive individuals who were also surveyed for stress-producing events occurring during the previous week. A positive polymerase chain reaction (PCR) signal was given in 104/127 (81.9%) urines, 73/127 (57.3%) throat washings and 68/127 (53.6%) leukocyte samples. An association was found between HCMV DNA in urine and a stress-producing event at work (p < 0.04). An association was also found between detection of HCMV DNA in throat washings and alcohol ingestion (P < 0.0061 and between the presence of oral herpes lesions and the detection of HCMV DNA in leukocytes (p c 0.0019). The results suggest that viral reactivation is more common than previously thought and that stress may be a triggering event.
Journal of Clinical Microbiology
The presence of human cytomegalovirus (HCMV) DNA in blood was investigated by the polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised patients. Of the 86 patients, 23 underwent clinical and virologic follow-up for HCMV infection. In parallel, blood samples were examined for viremia and antigenemia. Concordant results between PCR and assays for viremia and antigenemia were obtained on 124 positive and 110 negative samples, with an overall concordance of 79.8%, while 59 samples (most from patients with HCMV infection) were positive by PCR alone. PCR is a new powerful tool for detection of HCMV infections in blood samples from immunocompromised patients. However, its clinical significance appears to be restricted to the indication of a risk of reactivation of HCMV infection.