Synergistic Interaction between Hydroalcoholic Extracts of Aerial Parts of Tridex Procumbens L. and Bryophyllum Pinnatum L. Leaves to Promote In Vitro Hypoglycemic Activity (original) (raw)
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International Journal of Carbohydrate Chemistry, 2014
In the present work, we have studied the inhibitory effects of aqueous and alcoholic extracts of six Algerian medicinal plants known by their therapeutic virtues against diabetes. The total phenolic compounds content, assayed using Folin-Ciocalteu’s reagent, of the samples ranged from 0.183 mg/g to 43.088 mg/g and from 1.197 mg/g to 7.445 mg/g, expressed as gallic acid equivalent (GAE), for the, respectively, whereas the total flavonoids concentrations, detected using 2% of the aluminium chloride, ranged from 0.41 mg/g to 11.613 mg/g and from 0.0097 mg/g to 1.591 mg/g, expressed as rutin equivalents (RE), for the aqueous and methanolic extracts, respectively. The major plants were found to inhibit enzymatic activities of Aspergillus oryzae-amylase in a concentration dependent manner. The values of the inhibition constants (Ki) have been determined according to the Dixon and Lineweaver-Burk methods. The results showed that the Ki values were less than 55 ppm for the all extracts. A s...
Pharmaceutical Chemistry Journal
Diabetes mellitus is the metabolic disease leading to elevation of blood glucose, which may be due to the lack of enough insulin or responsible tissue become insulin resistance. The main aim of the present study was to evaluate in-vitro glucose diffusion and alpha-amylase inhibition as well as to determine free radical scavenging effects of selected plant sample. The five different plants i.e., Amomum subulatum Roxb., Choerospondias axillaris (Roxb.) B.L. Burtt, Nephrolepsis cordifolia (L). C. Presl, Musa sp. And Myrica esculenta Buch.-Ham. ex D. Don were collected from different area of Kaski district, Nepal. Myrica esculenta stem bark showed potent DPPH free radical scavenging effect in both solvent which showed IC50 value of 5.920µg/ml. Amomum subulatum, Choerospondias axillaris, Nephrolepsis cordifolia fruit and leaves and Musa species leaves in water have IC50 value of 0.81 mg/ml, 0.69mg/ml, 0.49mg/ml, 0.77mg/ml and 0.61mg/ml showing better alpha amylase inhibition. Glucose diffusion inhibition study revealed that Amomum subulatum seed, Choerospondias axillaries fruit and Musa species. Leaves showed highest GDRI% in water at concentration of 20mg/ml and 40 mg/ml. Thus, Myrica esculenta stem bark, Choerospondias axillaris fruit and Musa species leaves were found potent antioxidant and alpha amylase inhibitors.
Mini Reviews in Medicinal Chemistry, 2010
The inhibition of alpha-glucosidase and alpha-amylase, enzymes involved in the digestion of carbohydrates, can significantly reduce the post-prandial increase of blood glucose and therefore can be an important strategy in the management of blood glucose level in type 2 diabetic and borderline patients. Currently, there is renewed interest in plant-based medicines and functional foods modulating physiological effects in the prevention and cure of diabetes and obesity. The plant kingdom is a wide field to search for natural effective oral hypoglycaemic agents that have slight or no side effects. More than ca. 1200 plant species have been recorded to be used empirically worldwide for their alleged hypoglycaemic activity. Therefore, natural alpha-glucosidase and alpha-amylase inhibitors from plant sources offer an attractive strategy for the control of hyperglycaemia. This article reviews recent data on plant extracts and isolated natural compounds that are being tested for their hypglycaemic activity, highlights ongoing research and considers the future persepctives.
EFFECT OF COMBINATION OF TWO PLANT EXTRACTS ON DIABETES MELLITUS
International Journal of Pharmacy and Pharmaceutical Sciences, 2018
Objective: To investigate the anti-diabetic activity of combined ethanolic extracts (1:1mixture) of dry leaves of Syzygium cumini and Psidium guajava belonging to the family Myrtaceae as well as to compare the anti-diabetic activity of these plants by in vitro methods. Methods: In vitro glucose uptake assay was performed on cultured L6 cell lines (rat myoblast cell line) and estimated the glucose uptake using high sensitivity glucose oxidase kit. In vitro alpha amylase inhibitory assay was performed on porcine alpha amylase and the absorbance was measured at 540 nm using a microplate reader. Acarbose was used as the standard in both the methods. Results: At a concentration of 100µg/ml the percentage glucose uptake by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves was 43.95 while for acarbose the corresponding value was 51.71. At 100 μg/ml the percentage of glucose uptake by Syzygium cumini and Psidium guajava was 27.62 and 22.17 respectively. The percentage inhibition of alpha amylase by the combined ethanolic extract (1:1 mixture) of Syzygium cumini and Psidium guajava leaves at a concentration of 1000 µg/ml was 36.51 and it was 29.26 for Syzygium cumini and 23.43 for Psidium guajava. For acarbose the percentage inhibition of alpha amylase was 73.82 at the concentration of 1000 µg/ml. Conclusion: The combined extract of the leaves of the plants selected was found to be more effective than individual plant extracts against diabetes. The percentage glucose uptake of the combined extract was found to be closer to that of the standard drug acarbose. On comparison of two plants Syzygium cumini was found to be more active against diabetes than Psidium guajava. As the 1:1 mixture of the ethanolic extract is found to be more active, the combination of the two plants can be used to formulate drugs for treating diabetes.
The Scientific World Journal, 2012
We hypothesized that some medicinal herbs and food plants commonly used in the management of diabetes can reduce glucose peaks by inhibiting key carbohydrate hydrolyzing enzymes. To this effect, extracts ofAntidesma madagascariense(AM),Erythroxylum macrocarpum(EM),Pittosporum senacia(PS), andFaujasiopsis flexuosa(FF),Momordica charantia(MC), andOcimum tenuiflorum(OT) were evaluated for α-amylase and α-glucosidase inhibitory effects based on starch-iodine colour changes and PNP-G as substrate, respectively. Only FF and AM extracts/fractions were found to inhibit α-amylase activity significantly (P<0.05) and coparable to the drug acarbose. Amylase bioassay on isolated mouse plasma confirmed the inhibitory potential of AM and FF extracts with the ethyl acetate fraction of FF being more potent (P<0.05) than acarbose. Extracts/fractions of AM and MC were found to inhibit significantly (P<0.05) α-glucosidase activity, with IC50comparable to the drug 1-deoxynojirimycin.In vivostud...
Evaluation of hypoglycemic and anti-hyperglycemic potential of Tridax procumbens (Linn.)
BMC Complementary and Alternative Medicine, 2009
Background: Diabetes is a metabolic disorder affecting carbohydrate, fat and protein metabolism. Tridax procumbens Linn. (Family-Asteraceae; common name-Dhaman grass) is common herb found in India. Traditionally, the tribal inhabitants of Udaipur district in Rajasthan (India) uses the leaf powder (along with other herb) orally to treat diabetes. There is a need to evaluate extracts of this plant in order to provide scientific proof for it's application in traditional medicine system. Methods: Extraction of whole plant of T. procumbens using 50%methanol. The extract was tested for acute and sub-chronic anti-hyperglycemic activity in alloxan induced diabetic rats and for acute toxicity test among normal rats. Observations on body weight as well as on the oral glucose tolerance levels were also recorded. Results: Oral administration of acute and sub chronic doses (250 and 500 mg/kg b.wt.) of T. procumbens extract showed a significant (p < 0.05) reduction in fasting blood glucose levels in diabetic rats, however the decline in blood sugar levels in normal rats was not observed. In acute study the maximum percent blood glucose reduction (68.26% at 250 mg/kg and 71.03% at 500 mg/ kg body weight) in diabetic rats was observed at 6 h. The anti-hyperglycemic effects were not dependent of dose and the OGTT and Body weight supported the antihyperglycemic action of the drug. The results of anti-diabetic effect of T. procumbens were compared with the reference standard drug Glibenclamide (10 mg/kg b.wt.). Conclusion: These test results support traditional medicinal use of, T. procumbens for the treatment of diabetes mellitus with corrections in body weight and oral glucose tolerance and no visible signs or symptoms of toxicity in normal rats indicating a high margin of safety. These results warrant follow-up through bioassay-directed isolation of the active principles.
Phytochemical Analysis, Antidiabetic Potential and in-silico Evaluation of Some Medicinal Plants
Pharmacognosy Research, 2021
Background: The increasing frequency of diabetes patients and the reported side effects of commercially available anti-hyperglycemic drugs have gathered the attention of researchers towards the search for new therapeutic approaches. Inhibition of activities of carbohydrate hydrolyzing enzymes is one of the approaches to reduce postprandial hyperglycemia by delaying digestion and absorption of carbohydrates. Objectives: The objective of the study was to investigate phytochemicals, antioxidants, digestive enzymes inhibitory effect, and molecular docking of potent extract. Materials and Methods: In this study, we carry out the substratebased α-glucosidase and α-amylase inhibitory activity of Asparagus racemosus, Bergenia ciliata, Calotropis gigantea, Mimosa pudica, Phyllanthus emblica, and Solanum nigrum along with the determination of total phenolic and flavonoids contents. Likewise, the antioxidant activity was evaluated by measuring the scavenging of DPPH radical. Additionally, anti...
BMC Complementary and Alternative Medicine, 2012
Background Traditional Indian and Australian medicinal plant extracts were investigated to determine their therapeutic potential to inhibit key enzymes in carbohydrate metabolism, which has relevance to the management of hyperglycemia and type 2 diabetes. The antioxidant activities were also assessed. Methods The evaluation of enzyme inhibitory activity of seven Australian aboriginal medicinal plants and five Indian Ayurvedic plants was carried out against α-amylase and α-glucosidase. Antioxidant activity was determined by measuring (i) the scavenging effect of plant extracts against 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and 2, 2′-azinobis-3-ethylbenzothiazoline-6-sulfonate (ABTS) and (ii) ferric reducing power. Total phenolic and total flavonoid contents were also determined. Results Of the twelve plant extracts evaluated, the highest inhibitory activity against both α-amylase and α-glucosidase enzymes was exerted by Santalum spicatum and Pterocarpus marsupium with IC50 values of ...
Diabetes mellitus (DM) is one of the non-communicable life threatening diseases and oxidative stress has been identified to play a major role in the pathogenesis of different clinical conditions such as diabetes. Bryophyllum pinnatum leaves are employed as food and as traditional medicines. This study investigates the antioxidant potentials of the ethanolic extract of the leaves of Bryophyllum pinnatum and the effect on carbohydrate metabolizing enzymes. The antioxidants potentials were evaluated by determining the Total phenolic, flavonoid content and free radical scavenging effects. Its inhibitory effect on α-amylase and α-glucosidase activities were also investigated. The results of the study indicated that Bryophyllum pinnatum leaves contained a considerably high amount of Total Phenol with the highest value (101.2 ± 2.83mg/g GAE) at concentration of 1mg/ml and Total Flavonoids has the highest value(45.8.2 ± 2.82mg/g GAE) at 0.5mg/ml when compared with other tropical plants. The ethanolic extracts was able to scavenge free radicals in a dose dependent manner. Results also showed that ethanolic extract of Bryophyllum pinnatum had a considerable inhibitory effect on α-amylase and α-glucosidase activities at different concentrations when compared with a standard. This study suggest that ethanolic extract of Bryophyllum pinnatum leaves could be considered as potential source of bio-active constituents with excellent antioxidant and anti-diabetic activity.