Production of acidic lipase by Aspergillus niger in solid state fermentation (original) (raw)
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International Journal of Food Science & Technology, 2014
Lipase from Aspergillus sp. obtained by solid-state fermentation (SSF) on wheat bran (LWB), soybean bran (LSB) and soybean bran combined with sugarcane bagasse (LSBBC) were 67.5, 58 and 57.3 U of crude lipase per gram substrate, respectively. The optimum pH of activity and stability of the LWB was between 8 and 9, and the optimum temperature of activity and stability was 50°C and up to 60°C, respectively. The LSB and LSBBC showed two peaks of optimum pH (4 and 6) and optimal values of temperature and stability at 50°C. The LSB was stable in the pH range of 6-7, while LSBBC in the range of pH 4-7. All the enzymes show activities on p-nitrophenyl esters (butyrate, laurate and palmitate). LWB stood out either on the hydrolysis of sunflower oil, presenting 66.1% of the activity over commercial lipase and on the esterification of oleic acid and ethanol, surpassing the activities of the commercial lipases studied. The thin layer chromatography showed that LWB and LSB have produced ethyl esters from corn oil, while LWB produced it from sunflower oil.
International journal of pharma and bio sciences, 2013
Lipase production in Aspergillus niger was tested using both submerged fermentation (SmF) and solid-state fermentation (SSF) on a mineral culture medium and wheat bran, respectively.Culture media was optimized in both SmF and SSF. We found 1.46 IU/mL was the optimum activity of lipase in case of submerged fermentation, when medium containing 2% glucose and 2% olive oil under the conditions of 1 vvm and 450 m -1. However, 9.14 IU/g of dry solid substrate equivalent to 4.8 IU/mL of lipase activity was reached using solid-state fermentation process with a medium containing 0.75 % of ammonium sulphate and 0.34 % of urea. The optimum pH and temperature for enzymatic activity were pH=6 and 40 °C, respectively. When we used neutral and midly acid media, temperature ranges between 20 to 30⁰C for 24 hour, the enzyme showed 80% of its initial activity.
Investigation of low-temperature lipase production and enzymatic properties of Aspergillus Niger
Iranian Journal of Chemistry & Chemical Engineering-international English Edition, 2021
To obtain the optimum fermentation medium and circumstances for extracellular lipase construction (as an important biocatalyst and promising industrial enzyme) by Aspergillus Niger, the fermentation conditions of Aspergillus Niger were optimized by single factor and response surface design, the enzymatic properties of the crude enzyme were studied. The results displayed that the optimum fermentation medium was soluble starch 4%, (NH4)2SO4 0.1%, K2HPO4 0.1%, MgSO4·7H2O 0.05%, peptone 3%, olive oil 1.05% and initial pH 7. The optimal fermentation conditions were 30℃, the sample size was 26 mL/250 mL and the shaking speed was 213 r/min. The optimized lipase activity was 1.55 U/mL, which was 7.75 times of the pre-optimized lipase. It was found that when the pH value of lipase was 7.0, the activity of lipase reached its maximum value of 79.3±6.82%. When pH value was between 6.0 and 8.0, the activity of lipase could be kept above 60% and the stability was good. At the same time, through t...
2004
Of the 34 fungal species, isolated from a number of oily substrates, 9 exhibited lipase activity. AU 15, identified as Aspergillus sp., was found to be excellent lipase producer in submerged fermentation and was selected for solid-state fermentation (SSF). Among substrates like oil cakes of coconut, groundnut and sesame, wheat rawa, bombay rawa and soya beans (crushed), wheat rawa showed the highest lipase activity. The maximum enzyme yield (1934 U/g) was obtained with basal medium containing wheat rawa, olive oil and corn steep liquor, at 80% moisture content, pH 7.0 and 96 hrs incubation.
Biomedicine and Biotechnology, 2014
An enzyme with various commercial purpose, lipase is a carboxy esterase enzyme with many uses in different industries. Multiple isolates of Aspergillus niger were isolated from oil contaminated soil samples and screened for lipase producing ability on tributyrin medium. The isolate showing maximum activity was identified and subjected to growth parameters optimization in attempt to increase the enzyme producing ability of the isolate in larger scale. Different media with varying composition were examined for best lipase production. The activities of the lipase produced by the fungus at various pH were assessed. The enzyme activity was determined by the titration method. Maximum lipase activity of 2.4 U/ml was achieved with organic nitrogen rich media (designated as PM II) at pH 7 on the sixth day of culture. The lipase production was scaled up on a pilot scale in a 5 Liter fermenter maintaining growth parameters of pH 7, temperature at 28°C, stirrer rate at 120 rpm, airflow rate at 30 L/hr, O2 saturation 50% and pressure 0.05 MPa. The crude enzyme was extracted for further assays. Optimization of the parameters can improve the productivity as well as the quality of the enzyme produced.
Indonesian Journal of Biotechnology, 2017
Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potentialas a medium for lipase production. The objective of this research was to characterize lipase from Aspergillusniger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate specifcity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate specifcity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and vmax about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipas...
Production and optimization of lipase using Aspergillus niger MTCC 872 by solid-state fermentation
Bulletin of the National Research Centre
Background: Lipases are serine hydrolases that degrade triglycerides, an attribute that treasures wide applications in biodiesel production, detergent, chemical industries, etc. The most sought after the application is in the high quality and economical production of biodiesel under mild reaction conditions and simplified product separation. For the said application, fungal lipases are ideal catalysts that could effectively catalyze esterification and transesterification reactions with their specific ability to release fatty acids from 1, 3 positions of acylglycerols. Results: In the present work, to facilitate bulk synthesis, lipase production using Aspergillus niger MTCC 872 was studied by solid-state fermentation (SSF). The chosen fungal strain was evaluated for lipase production using a mixture of agroindustrial substrates viz. rice husk, cottonseed cake, and red gram husk in various combinations at flask level. Tri-substrate mixture (rice husk, cottonseed cake, and red gram husk) combined in the ratio of 2:1:1 has shown the maximum lipase activity 28.19 U/gds at optimum cultivation conditions of temperature 40°C, moisture content 75% (v/w), pH 6.0 and initial spore concentration of 5.4 million spores per mL. Further studies were performed for scale-up of lipase from flask level to lab scale using tray fermenter. Lipase activity was found to be 24.38 U/gds and 21.62 U/gds for 100 g and 1000 g substrate respectively. Conclusion: This is the first report on the production of lipase from Aspergillus niger MTCC 872 using tri-substrate mixture of rice husk (RH), cottonseed cake (CSC), and red gram husk (RGH). Moreover, comparison between individual, binary, and tri-substrate mixture was carried out for which the highest lipase activity was observed for tri-substrate mixture. In addition, comparable results were found when scale-up was performed using tray fermenter. Thus, the current work signifies usage of agro-industrial residues as substrates for enzyme production by solid-state fermentation process as an effective alternative to submerged fermentation for industrial applications.
ABSTRACT Extracellular lipase production by Penicillium chrysogenum, Trichoderma harzianum and Aspergillus flavus was carried out through solid state fermentation using agro-industrial residues as substrates. For all three strains, the growth temperature was 29±1 °C, and 65 % w (g/gds) moisture content. The effect of three factors on lipase production rate was investigated: initial pH (6.0 and 7.0), time of fermentation (72 h, 96 h and 120 h), and type of mixed substrate (wheat bran-olive oil, and wheat bran-castor oil cake). The process was optimized applying a mixed level factorial design. Fermentation time and pH were found to have positive effects on lipase production and secretion rates. However, the time effect was larger than initial pH. Type of substrate demonstrated minor effective importance than the other two factors, and Aspergillus flavus showed the larger lipase production among the three strains. Results indicated that the three fungal strains were able to grow and produce lipase in both culture mediums. The maximum lipase activity achieved was 121.35 U/gds by Aspergillus flavus, which was five and nine times the lipase produced by Trichoderma harzianum and P. chrysogenum respectively, at the same conditions. An initial neutral pH and 96 h of fermentation time were the optimum conditions for lipase production by Aspergillus flavus.
Journal of Basic Microbiology, 2010
Extracellular lipase production by Aspergillus sp. (RBD-01) was monitored by modulating pH of the growth medium, ambient temperature for growth, source of nitrogen and percentage of carbon (virgin cottonseed oil). This strain was observed to be viable and produces lipase even up to 50% oil as a main carbon source. Maximum lipase activity of 21.8 U/ml was obtained with 50% (v/v) oil acting as the main carbon source and peptone (0.5% w/v) as nitrogen source. The optimum pH and temperature for enzymatic activity were observed to be 7.5 and 35 °C, respectively. The observations are of significance due to limited reports on use of 50% of oil as the main carbon source while obtaining significant lipase activity of 21.8 U/ml.
A comparative study of solid-state fermentation (SSF) and submerged fermentation (SmF) for the production of alkaline lipase from a new isolate, Aspergillus fumigatus MTCC 9657, using defatted rice bran (DFRB) as a substrate was studied. Diff erent process parameters, such as incubation period, initial pH and incubation temperature, were optimised to achieve the maximum yield of the alkaline lipase. Maximum enzyme production (8.13 U/mL) was obtained by day 7 of incubation in SSF compared to day 4 in SmF at an optimum pH of 8.5 and ambient temperature of 30 °C. Lipase produced by SSF was stable over a period of 15 days, whereas lipase production in SmF decreased by day 5. Lipase production in SSF with DFRB using sterile water as a moisture source exhibited more activity (577.5 U/70 mL) than that supplemented with mineral medium. Compared to the cost of culture medium, the solid-state substrate DFRB is inexpensive, and therefore this process is industrially and economically feasible. Th ese results confi rm the interesting potential of SSF in DFRB without any additional nutritional supplement.