Synthesis, characterization and biological activities of cephalosporin metals complexes International Journal of Biosciences | IJB (original) (raw)
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Current Analytical Chemistry, 2010
Cefaclor (CEF), a second generation cephalosporin antibiotic, possesses various donor sites for interaction with transition metal (II) ions such as Cu(II), Co(II) and Ni(II) to form complexes of the type [M(CEF)(H 2 O)Cl], with a molar ratio of metal:ligand (M:L) of 1:1. These complexes were prepared and characterized by physicochemical and spectroscopic methods. Their UV-Vis, IR and mass spectra suggest that CEF potentially acts as a bi-dentate ligand. The electrochemical behavior of these synthesized and in solution complexes is studied over glassy carbon electrode in various buffer solutions using cyclic, linear sweep, differential pulse (DP) and square wave (SW) voltammetric techniques. CEF enrichment is observed over Cu(II) complex. The peak current and peak potential of the complex depend on pH, initial potential, and scan rate. DP and SW voltammetric techniques were used for the determination of CEF-Cu(II) complex. For solid synthesis complex, the linear response was within 1x10 -6 -3x10 -5 M with a detection limit on one decimal point: 2.26x10 -7 for DPV and 2.30x10 -7 M for SWV techniques in acetate buffer at pH 4.70. The repeatability of the methods was within 0.82-0.78% for peak potentials and 1.16-0.71% for peak currents. All necessary validation parameters were investigated as detailed in all media. The complexes have been screened for antibacterial activity and results were compared with the activity of the uncomplexed antibiotic against Pseudomonas aeruginosa, Kluvyeromyces fragilis, Saccharomyces cerevisiae, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Bacillus megaterium, Candida albicans, Mycobacterium smegmatis, Bacillus cereus, Enterococcus cloacae and Micrococcus leteus. The copper complex was found to be more potent against two bacterial species than the uncomplexed CEF.
The research work comprises of interaction studies of ceftriaxone with essential metal, antacid and investigation of antimicrobial activity of ceftriaxone. Ceftriaxone is included among the cephalosporins drug class which is active against a wide range of gram positive and negative bacteria. Since the presence of compelling ligand may affect the bioavailability of a metal in the blood or tissues, therefore in order to study the portable interaction of ceftriaxone with essential and trace elements present in the body. Ceftriaxone has been interacted with Zn (metal), Ca (antacid) as an in-vitro analysis. All the reaction conditions were simulated to natural environment. Also the anti-microbial activity of the drug and the complexes were determined. There is an effect of pH on drug metal complexation. It has observed that ceftriaxone interacts with metal on a pH 7.4, the stability constant of these complexes were determined in order to evaluate their possible in vivo implications. This research work confirms that there was a possible interaction between the ceftriaxone and metal Zn and antacid Ca which was confirms by jobs plot method and by antimicrobial investigation it was confirmed that the zone of inhibition of ceftriaxone with Metal Zn and antacid Ca reduced from 16 mm to 14mm & 11mm respectfully. The standard ceftriaxone disk also tested against Staphylococcus aureus. In order to investigate the number of metal ions involved in the complexation with ceftriaxone complexes were elucidated by plotting various UV spectrophotometric methods. The ultraviolet studies of these complexes were carried out and compared.
ANTIBACTERIAL STUDIES OF CEFIXIME COPPER, ZINC AND CADMIUM COMPLEXES
2002
Antibacterial studies of metal complexes of cefixime, with copper zinc and cadmium have been carried out by observing the Minimum Inhibitory Concentration (MIC) and by measuring the Zone of Inhibition of the complexes and compared with the parent cephalosporin against both Gram negative and Gram positive microorganisms. Various microorganisms used were Staphylococcus aureus, E. coli, Klebsiella and Proteus species. For MIC observation, serial dilution method was employed and zone sizes were determined by diffusion disk method. Our investigations divulge that formation of complexes results in decrease in antimicrobial activity of cefixime and MIC values were increased.
Synthesis and antibacterial activity of cefoxitin metal complexes
Transition Metal Chemistry, 2005
Cefoxitin (Hcefoxi) interacts with transition metal ions to give [M(cefoxi)Cl] complexes [M ¼ Fe(III), Co(II), Ni(II), Cu(II), Zn(II) and Cd(II)] which were characterized by physicochemical and spectroscopic methods. A tetrahedral geometry is suggested for their structure, where the cefoxitin behaves as a monoanionic tridentate ligand. The complexes have been screened for antibacterial activity against several bacteria, and the results show that they are less active than the parent cefoxitin.
Synthesis and Antibacterial Activity of Metal Complexes of Cephalothin
Journal of Coordination Chemistry, 2003
Microbes are suitable candidates to recover and decontaminate different environments from soluble metal ions, either via reduction or precipitation to generate insoluble, non-toxic derivatives. In general, microorganisms reduce toxic metal ions generating nanostructures (NS), which display great applicability in biotechnological processes. Since the molecular bases of bacterial reduction are still unknown, the search for new-environmentally safe and less expensive-methods to synthesize NS have made biological systems attractive candidates. Here, 47 microorganisms isolated from a number of environmental samples were analyzed for their tolerance or sensitivity to 19 metal(loid)s. Ten of them were highly tolerant to some of them and were assessed for their ability to reduce these toxicants in vitro. All isolates were analyzed by 16S rRNA gene sequencing, fatty acids composition, biochemical tests and electron microscopy. Results showed that they belong to the Enterobacter, Staphylococcus, Acinetobacter, and Exiguobacterium genera. Most strains displayed metal(loid)-reducing activity using either NADH or NADPH as cofactor. While Acinetobacter schindleri showed the highest tellurite (TeO 2− 3) and tetrachloro aurate (AuCl − 4) reducing activity, Staphylococcus sciuri and Exiguobacterium acetylicum exhibited selenite (SeO 2− 3) and silver (Ag +) reducing activity, respectively. Based on these results, we used these bacteria to synthetize, in vivo and in vitro Te, Se, Au, and Ag-containing nanostructures. On the other hand, we also used purified E. cloacae glutathione reductase to synthesize in vitro Te-, Ag-, and Se-containing NS, whose morphology, size, composition, and chemical composition were evaluated. Finally, we assessed the putative anti-bacterial activity exhibited by the in vitro synthesized NS: Te-containing NS were more effective than Au-NS in inhibiting Escherichia coli and Listeria monocytogenes growth. Aerobically synthesized Figueroa et al. Biosynthesis of Metal(loid)-Containing Nanostructures TeNS using MF09 crude extracts showed MICs of 45-and 66µg/ml for E. coli and L. monocytogenes, respectively. Similar MIC values (40 and 82 µg/ml, respectively) were observed for TeNS generated using crude extracts from gorA-overexpressing E. coli. In turn, AuNS MICs for E. coli and L. monocytogenes were 64-and 68µg/ml, respectively.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2011
Iron, cobalt, nickel and copper complexes of ceftriaxone were prepared in 1:3 ligand:metal ratio to examine the ligating properties of the different moieties of the drug. The complexes were found to have high percentages of coordinated water molecules. The modes of bonding were discussed depending on the infrared spectral absorption peaks of the different allowed vibrations. The Nujol mull electronic absorption spectra and the magnetic moment values indicated the Oh geometry of the metal ions in the complexes. The ESR spectra of the iron, cobalt, and copper complexes were determined and discussed. The thermal behaviors of the complexes were studied by TG and DTA techniques. The antimicrobial activities of the complexes were examined and compared to that of the ceftriaxone itself.
Synthesis and Antibacterial Activities of Metal Complexes of Ceftiofur
Rasayan Journal of Chemistry
The objective of the present study is to prepare a family of new monoanionic tridentate Ceftiofur metal complexes [M(Ceftiofur)Cl].xH2O, where M = Mn(II), Ca(II), Fe(III), Cu(II), Zn(II), Mg(II), Co(II) and Ni(II) and to characterize them by different physiochemical techniques. These Ceftiofur metal complexes are further screened for biological activities. Several physiochemical and spectroscopic methods like UV-Vis, 1 H-NMR, IR, elemental analysis, and Karl-Fischer titration were used to find out the actual stoichiometry and binding mode of these complexes. The complexes synthesized were subjected to anti-microbial activities using gram-positive as well as gram-negative strains. The chosen strains are Staphylococcus aureus (MTCC87), Bacillus subtilis (MTCC441), Escherichia coli (MTCC40), Proteus vulgaris (MTCC426), Pseudomonas aeuruginosa (MTCC2488), Bacillus cerus (MTCC430), Salmonella typhimurium (MTCC1253). The solubility of Ceftiofur hydrochloride was remarkably decreased in most of the commonly used solvents on its complexation with metal ions under study. The stoichiometric study of these synthesized complexes revealed 1:1 ratio of ligand to metal binding. Ceftiofur showed that on complexation the antibacterial study was affected. The Cu 2+ and Co 2+ complexes against Pseudomonas aeuruginosaand Co 2+, Mg 2+ and Ni 2+ complexes againstSalmonella typhimurium showed the highest antibacterial activity, whereas Ca 2+ complex showed the highest activity against Proteus vulgaris. Majority of the bacterial strains were found to be resistant to the complexes of Fe 3+ and Zn 2+ .
Transition Metal Chemistry, 2008
Copper(II) reacts with cephalosporins plus sulphathiazole (Hstz) to form the following mixed complexes: [Cu(cefazol)(stz)(H 2 O)], [Cu(cephalot)(stz)(H 2 O) 2 ], [Cu (cefotax)(stz)], [Cu(ceftria)(Hstz)] and [Cu(cefepime)(stz)Cl] (Hcefazol = cefazolin, Hcephalot = cephalothin, Hcefotax = cefotaxime and H 2 ceftria = ceftriaxone) which were characterized by physicochemical and spectroscopic methods. The spectra indicated that most of the cephalosporins are probably acting as monoanionic multidentate chelating agents, the exception being ceftriaxone which is dianionic. The complexes are insoluble in water and common organic solvents and probably have polymeric structures. They have been screened for activity against several bacteria, and the results are compared with the activity of cephalosporins.
2014
Cefodroxil (CEFDX) is a broad spectrum cephalosporin belonging to the first generation antibiotic agents. In this study, attention has been paid to the synthesis, characterization and biological evaluation of new Co II and Ni II complexes of CEFDX. The stoichiometrics and the mode of bonding of the complexes were deduced from their elemental and electrical conductivity measurements. For the characterization of the metal complexes various techniques like FTIR, UV-Vis Spectral study and TG-DSC were used. The composition of the complexes was identified as 1:1 ratio. Based on the study of infrared spectra, coordination through amide group and carboxylate group is proposed. Octahedral structures were proposed for these complexes depending upon the electronic spectral data. The thermal analysis data shows that water molecules have coordinate bonds with transition metal ion. Antimicrobial activity of thecomplexes was determined using Gram-positive strains of (Staphylococcus aureus) and Gram-negative strains of (Escherichia coli) bacteria by disc diffusion method.The antibacterial study of ligand and metal complexes were found to be better than the parent antibiotic.