Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil (original) (raw)
Related papers
Diagnosis of Encephalitic Listerial Outbreak in Sheep-Immuno-Histopathological Study
International Journal of Current Microbiology and Applied Sciences, 2020
Listeriosis caused by Listeria monocytogenes is an OIE enlisted infectious disease affecting ruminants, monogastric animals and man. The infection is usually manifested by three distinct non-overlapping clinical syndromes, namely: meningoencephalitis, septicaemia and abortions 1 . Encephalitic listeriosis is more prevalent among ruminants, especially sheep. Encephalitic form resulting from abrasions of the buccal mucosa most likely follows an ascending infection along trigeminal nerve to the brain stem 2,3 . Meningoencephalitis caused by listeria produces characteristic lesions of micro-abscesses in the brain stem. The disease has been reported in small ruminants in an outbreak form in Punjab 4, 5 . The present paper reports the outbreaks of listeriosis in migratory flock of sheep and goat in Punjab and describes the clinical features, histopathological changes and confirmation by immunohistochemistry. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-770...
Pesquisa Veterinária Brasileira
ABSTRACT: Listeriosis is an infectious disease caused by bacteria of the genus Listeria, the neurological form being more common in ruminants. There are many reports of listeriosis in small ruminants in the region that includes Brazil, Argentina and Uruguay. However, these diagnoses were mainly based on histological lesions in the central nervous system (CNS) without the isolation and characterization of the involved Listeria strains. The aim of this study was to report sheep and goats listeriosis cases from 2016 to 2021 in northwestern Uruguay. The diagnosis was made according to lesions observed at histopathology, plus Listeria isolation in CNS, identifying it at specie and serotype level. Nine animals (n=9) of three outbreaks and five sporadic cases of listeriosis were studied. Sheep was the species with more cases in relation to goats, and adults were the category most affected. Cases occurred in spring and less frequently in winter. All presented neurological clinical signs and...
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2022
Encephalitic listeriosis is the most significant purulent encephalitis in ruminants and is a very common endemic problem in sheep, cattle, and goats. In this study, it was aimed to compare the presence of Listeria (L.) monocytogenes revealed by immunohistochemical (IHC) and Real-Time PCR methods with histopathological findings obtained from the archive materials. The study material consisted of pons and medulla oblongata paraffin tissue of 100 ruminants (9 cattle, 4 calves, 44 sheep, 38 lambs, and 5 goats). Positivity was obtained by the IHC method in 46 (46%) and by the Real-Time PCR method in 21 (21%) of 100 cases. In the L. monocytogenesis antigen IHC scoring, more severe staining was observed in sheep and goats (P>0.05). In the IHC positive cases, microabscess was more severe in sheep and goats than in cattle and lambs (P<0.05). In addition, 19 patients had Coenurus cerebralis cysts, and 3 of them were found to be positive for the IHC agent of Listeria. It was concluded that IHC and PCR methods can be used to detect L. monocytogenes from paraffin blocks, but the IHC method is a more effective method than PCR in revealing the presence of antigen from paraffin blocks stored for many years.
Investigation of Ruminant Encephalitic and Septicemic Listeriosis by the Immunofluorescence Method
2021
Listeria genus is Gram-positive facultative intracellular bacteria that are found in the soil as saprophytes but also contain species that have pathogenic effects in their last host. Listeria monocytogenes is the major pathogen in Listeria species that is responsible for the majority of Listeriosis cases in humans and animals. In this study, it was aimed to investigate L. monocytogenes with histopathological and immunofluorescence (IF) methods in brain and liver tissues taken from sheep and cattle with clinical signs of suspicious Listeriosis. In the study, isolation and identification of L. monocytogenes with cultural methods were also applied. The material of the study consisted of tissue samples from 16 sheep and 2 cattle. As a result of cultural analysis, L. monocytogenes was identified from 12 (66.6%) of cases. Within the framework of histopathological and macroscopic findings, 16 (88.8%) of 18 cases with suspected Listeriosis were positive with the immunofluorescence method. T...
Neuropathogenesis of Naturally Occurring Encephalitis Caused by Listeria monocytogenes in Ruminants
Brain Pathology, 2010
Listeriosis is a serious food-borne disease with increasing frequency in humans and ruminants. Despite the facts that in both hosts, listeriosis can occur as rhombencephalitis and ruminants are a reservoir of Listeria monocytogenes (LM) strains pathogenic for humans, little work has been done on the pathogenesis in ruminants. This study investigates the neuropathogenesis of listeric encephalitis in over 200 natural cases in cattle, sheep and goats by analyzing anatomical distribution, severity, bacterial load and temporal evolution of the lesions. Our results suggest that LM gains access to the brainstem of all three species via axonal migration not only along the trigeminal nerve, but also along other nerves. The ensuing encephalitis does not remain restricted to the brainstem. Rather, LM spreads further from the brainstem into rostral brain regions likely by intracerebral axonal migration. Significant differences in severity of the lesions and bacterial load were found between cattle and small ruminants, which may be caused by species-specific properties of antibacterial immune responses. As histopathological lesions of human rhombencephalitis caused by LM strongly resemble those of ruminants, the disease likely has a similar pathogenesis in both hosts.
Pathological and epidemiological investigations into listerial encephalitis in sheep
Small Ruminant Research
This article was originally published in a journal published by Elsevier, and the attached copy is provided by Elsevier for the author's benefit and for the benefit of the author's institution, for non-commercial research and educational use including without limitation use in instruction at your institution, sending it to specific colleagues that you know, and providing a copy to your institution's administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access, or posting on open internet sites, your personal or institution's website or repository, are prohibited. For exceptions, permission may be sought for such use through Elsevier's permissions site at: http://www.elsevier.com/locate/permissionusematerial Abstract Three outbreaks of encephalitis caused by Listeria monocytogenes were investigated in the migratory flocks of sheep in Punjab, India. Sixty-nine sheep were affect...