POTENTIATING EFFICACY OF ANTIBIOTIC CONJUGATES WITH ZINC OXIDE NANOPARTICLES AGAINST CLINICAL ISOLATES OF STAPHYLOCOCCUS AUREUS (original) (raw)
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International journal of drug delivery technology, 2022
INTRODUCTION Microparticle (MPs) use extended to biomedical applications, including antibiotic drugs for conventional uses in clinics, implants, and cosmetics. 1,2 Their particle size thereby influences antimicrobial activity against pathogenic microorganisms. 3 Delivery into tumor cells of ZnO NPs as carriers of antitumor drugs for targeted drug, on different types of cancers along with their mode of actions, 4,5 less cytotoxic metal oxide NPs of metallic oxide display promising prospects withinside the area of biomedicine. 6 There are many sorts of metallic oxides nanoparticles like ZnO, CuO, TiO 2 , Fe 2 O 3 , MgO, NiO, and ZrO 2 nanoparticles. 7 In scientific settings, this has a profound effect on the remedy of implant-related infections, as those are characterized by biofilm formation. 8 Different characterization nanocomposite materials such as physical and chemical NPs gave greater properties. 9 Staphylococcus aureus, even though commonly recognized as a commensal, human bacterial infection in which different gentle tissues include pores, skin, bones, and bloodstream. Antistaphylococcal antimicrobial drugs have higher resistance to every new category. 10 MATERIAL AND METHODS Zinc acetate heptahydrate (ZnSO 4 .7H 2 O) and (NaOH), used chemicals were employed without any treatment in this work. Synthesis of ZnO Nanoparticles Directly by precipitation, The ZnO-NPs were prepared from two aqueous solutions, NaOH and ZnSO 4 .7H 2 O. 11 The precursor solwution with vigorous stirring for 20 minutes. A solution with pH=7 containing a white precipitate can be observed as shown in Figure 1. was collected the white precipitate filtered with distilled water and washed several times. Characteristics of Synthesis Samples Techniques were used to conduct this work, such as Atomic absorption analysis, which was applied to estimate all ZnO NPs. Fourier transform infrared spectroscopy (FT-IR) and ABSTRACT A novel technique for nanoparticles with a chemical method and impact for resistance bacteria methicillin-resistant Staphylococcus aureus (MRSA), UV-visible analysis confirmed the by Fourier transform infrared spectroscopy (FT-IR) and Energy dispersive X-Ray (EDX), Scanning electron microscope (SEM) and X-ray diffraction pattern estimation antimicrobial excellent antibacterial activity against MRSA (with zone of inhibition of 11 ± 02 mm , 9 ± 01 mm,8 ± 03 mm and 7.5 ± 02 mm and 6.5 ± 02 mm) at different concentrations (0.5 ,0.25, 0.125, 0.0625, 0.03125) mg/ml while good activity was 16 ± 03 mm at 17 ± 02 mm zone at 0.25, 0.125 mg/mL, respectively. The increase in microorganism resistance to antibiotics a couple of have caused Antimicrobial factors are widely recognized (ZnO NPs) and are less toxic and biological safety. evaluation of MRSA by minimum inhibitory concentration (MIC) (0.5, 0.25, 0.125, 0.0625, 0.03125) mg/Ml of ZnO NPs. This research aims to study zinc oxide (ZnO) nanoparticles synthesis and antibacterial. In vivo evaluation precipitation method of cytotoxicity was determined WRL68 normal A375 cells.
Nanostructured zinc oxide enhances the activity of antibiotics against Staphylococcus aureus
J. Biosci. …, 2010
The synthesis and bioactivity of zinc nanoparticles has been extensively studied. However, the antibacterial activity of zinc nanoparticles individually or in combination with different antibiotics has not been demonstrated. In the present study the .effect of zinc nano particles on the antibacterial activity of different antibiotics was evaluated against Staphylococcus aureus. Disk diffusion method was used to determine the antibacterial activity of various classes of antibiotics in the absence and presence of sub inhibitory concentration of zinc nano particles. A clinical isolate of S. aureus was used as the test strain. In the presence of sub-inhibitory concentration of zinc nano particles (100µg/disc) the antibacterial activities of all antibiotics have. Increased from minimum 2 mm to a maximum of 10mm. The highest increase was observed for Penicillin G and Amikacin. These results signify that the zinc nano particles potentiate the antimicrobial action of beta lactums, cephalosporins, amino glycosides, glycopeptides, macrolids and lincosamides, tetracycline, suggesting a possible utilization of nano compounds in combination therapy against S. aureus.
AMB Express, 2021
At present, antibiotic resistance is one of the most pressing issues in healthcare globally. The development of new medicine for clinical applications is significantly less than the emergence of multiple drug-resistant bacteria, thus modification of existing medicines is a useful avenue. Among several approaches, nanomedicine is considered of potential therapeutic value. Herein, we have synthesized Zinc oxide nanoparticles (ZnO-NPs) conjugated with clinically-approved drugs (Quercetin, Ceftriaxone, Ampicillin, Naringin and Amphotericin B) with the aim to evaluate their antibacterial activity against several Gram-positive (Methicillin resistant Staphylococcus aureus, Streptococcus pneumoniae and Streptococcus pyogenes ) and Gram-negative ( Escherichia coli K1 , Serratia marcescens and Pseudomonas aeruginosa ) bacteria. The nanoparticles and their drug conjugates were characterized using UV-visible spectrophotometry, dynamic light scattering, Fourier transform infrared spectroscopy ...
Antibacterial activity of Zinc oxide nanoparticles on Staphylococcus aureus
2016
Introduction: One of the main healthproblemsis increasing resistance of bacteria to antimicrobial agents world-wide. Nanoparticles can be useful for preventing the distribution of resistant bacteria in the hospital and community. This study was carried out to determine the antimicrobial activity ofzinc oxide (ZnO) nanoparticle against isolated Staphylococcus aureus. Method of study:This study was conducted on 268 Staphylococcus aureuswhichwas isolated from healthy carriers, patients and food materials in Golestan province, innorth –east Iran.Antimicrobial activity of ZnOnanoparticles (10-30 nmdiameters) against Staphylococcus aureus was carried out by Agar dilution method and subsequently by Microdilution method for thedetermination of its minimum inhibitory concentration (MIC). Distribution of MIC were evaluated and statistically analyzed on the basis of source, antibacterialresistance, the ability of biofilm formation and virulence factors. Results: The minimum concentration of Zn...
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Staphylococcus aureus, as one of the main agentsfor hospital infections,is considered as highly important because they show resistance to a wide range of antibiotics. Resistance to selective antibiotics such as vancomycin is a serious problemin the medical community; thus it seems rational to use alternative substances for treating these bacteria. The aim of this study is to investigate the prevalence of resistance to vancomycin in clinical isolates of S. aureus as well as the antimicrobial effects of zinc oxide nanoparticles on them. In this study, 70 samples of wound, boil, abscess and urine were isolated.Staphylococcus aureus strains resistant to vancomycin were then identified through routine laboratory tests using Broth Micro dilution test. The antibacterial effect of ZnO nanoparticles (20 nanometer) was investigated at concentrations of 100, 50, 25, 12.5 and 6.25 mg/ml using agar well diffusion method over strains resistant to vancomycin. From the total of 70 samples, 30 sampl...
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Staphylococcus aureus (S. aureus) is a Gram-positive bacteria considered one of the leading causes of community and hospital-acquired illnesses or public health concerns. Antibiotic resistance in this microorganism is one of the greatest issues in global health care. The use of metal nanoparticles and their oxides is one of the potential approaches to combating bacteria resistance to antibiotics. The antibacterial properties of ZnO NPs against enterotoxigenic S. aureus were studied. ZnO NPs were tested in vitro by agar diffusion test. They resulted in 26 and 22 mm zones of inhibition for a size of 20 nm and a concentration of 20 mM against 105 and 107 CFU/mL S. aureus, respectively. The MIC of ZnO NPs of various sizes, 20 and 50 nm, with 105 CFU/mL was 2.5 and 5 mM, respectively. MIC with 107 CFU/mL was five mM for 20 and 50 nm ZnO NPs. Further, the highest growth reduction percentage, 98.99% in the counts of S. aureus was achieved by ZnO NPs of size 20 nm and concentration of 10 mM...
International Journal of Molecular and Clinical Microbiology, 2016
Staphylococcus aureus, as one of the main agentsfor hospital infections,is considered as highly important because they show resistance to a wide range of antibiotics.Resistance to selective antibiotics such as vancomycin is a serious problemin the medical community; thus it seems rational to use alternative substances for treating these bacteria.The aim of this study isto investigate the prevalence of resistance to vancomycin in clinical isolates of Staphylococcus aureus as well as theantimicrobial effects of zinc oxidenanoparticles on them. In this study, 70 samples of wound, boil, abscess and urine were isolated.Staphylococcus aureus strains resistant to vancomycin were then identified through routine laboratory tests using Broth Microdilution test. The antibacterial effect of ZnO nanoparticles(20 nanometer) was investigated at concentrations of 100,50,25,12.5 and 6.25 mg/ml using Agar well diffusion method over strains resistant to vancomycin. From the total of 70 samples, 30 sam...
Environmental Nanotechnology, Monitoring & Management, 2020
Synthesis of Zinc oxide nanoparticles and its conjugation with antibiotic: antibacterial and morphological characterization Pankaj Kumar Tyagi (Conceptualization) (Methodology) (Funding acquisition), Deepak Gola (Investigation) (Writing-original draft) (Writing-review and editing), Shruti Tyagi (Conceptualization) (Methodology) (Funding acquisition), Ankit Kumar Mishra (Investigation) (Writing-original draft) (Writing-review and editing), Arvind Kumar (Investigation) (Writing-original draft) (Writingreview and editing), Nitin Chauhan (Investigation) (Writing-original draft) (Writing-review and editing), Anami Ahuja (Validation) (Formal analysis), Sandeep Sirohi (Validation) (Formal analysis)
International journal of basic science in medicine, 2018
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International Journal of Biomedicine
Skin and soft tissue infections caused by methicillin-resistant S. aureus (MRSA) are among the most common bacterial infections. Linezolid is a cortisone drug for the treatment of infections caused by MRSA. However, developing resistance to linezolid creates a hurdle in the treatment of these infections. The present study aimed to determine the activity of linezolid alone and in combination with zinc oxide nanoparticles (ZnO-NPs) for the purpose of reducing resistance and enhancing its efficacy. For this study, MRSA isolates were taken and confirmed by using the antibiotic susceptibility testing method. The minimum inhibitory concentration (MIC) of both antibiotics and nanoparticles against MRSA clinical isolates was done by using the broth microdilution method. A checkerboard assay has used the determination of the combined activity of linezolid and ZnO-NPs. ZnO-NPs displayed a spherical shape with smooth surface morphology and had a mean size of 10 nm to 20 nm, with a zeta potenti...