Genetic and chemical variations among wild populations of a medicinal plant (Artemisia herba-alba Asso.) collected from different regions in Jordan (original) (raw)

Journal of Food Agriculture and Environment

Artemisia herba-alba Asso.(locally known as A'Shih) is a medicinal plant which is found under natural habitats in a wide range of environmental and climatic conditions of Jordan. Genetic variations and chemical compositions among and within the population of Artemisia herba-alba Asso. were studied in Jordan using Random Amplified Polymorphic DNA (RAPD) and Gas Chromatography/Mass Spectrometry (GC/MS). Four regions, namely Al-Shoubak, Al-Tafileh, Madaba and Al-Mafraq, were selected to study the variability among collected populations. Based on the presence of Artemisia herba-alba species, three monitoring areas were selected within the four regions in 2009. For RAPD molecular analysis purposes, five DNA samples were used per each population to study the genetic variability among and within A. herba-alba populations. Four primers were used based on the RAPD technique, which revealed a total number of 930 bands among which 117 are polymorphic. High polymorphism among studied popula...