Anti-Heat Shock Protein 27 Antibody Levels and Diabetes Complications in the EURODIAB Study (original) (raw)
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Cell Stress and Chaperones, 2010
Heat shock protein 27 (Hsp27) is over-expressed when cells are exposed to stressful conditions that include oxidative stress. Oxidative stress has been implicated in the pathogenesis of cardiovascular disease (CVD), diabetes and insulin resistance. We have investigated the concentrations of serum Hsp27 antigen and antibodies in subjects from different glycaemic categories, who either did or did not have established CVD. Serum Hsp27 antigen and antibody levels (immunoglobulins M and G (IgM and IgG)) were determined by enzyme-linked immunosorbent assays (ELI-SAs) in 68 individuals: 26 with normal glucose tolerance (NGT), 10 with (+) and 16 without (−) a history of CVD and 42 individuals with varying degrees of glucose intolerance (GI; 21 with and 21 without a history of CVD). Insulin sensitivity was determined in each subject using indices derived from the homeostasis model assessment of sensitivity and the insulin sensitivity index for glycaemia. Serum Hsp27 concentrations were significantly higher in GI (+CVD) subjects compared to GI (−CVD) subjects (p=0.03), NGT (−CVD) subjects (p=0.02) and NGT (+CVD) subjects (p =0.04) and were positively correlated to fasting plasma glucose for all subjects (r= 0.28, p=0.03). IgM antibody levels were significantly higher in GI (+CVD) subjects compared to NGT (−CVD) group (p=0.02) and were inversely related to fasting insulin concentrations (r=−0.27, p=0.04) and the 2-h insulin concentrations (r=−0.29, p=0.03) for all subjects. Serum IgG antibody levels were higher in GI (+CVD) group compared to GI (−CVD) group (p=0.06). In conclusion, Hsp27 and its antibody concentrations appear to relate to the presence of cardiovascular complications in patients with GI.
Serum anti-hsp27 antibodies concentration in diabetes mellitus; population based case-control study
Medical laboratory sciences, 2019
Background: Diabetes mellitus is an important risk factor for cardiovascular disease. Different biomarkers have been investigated for the diagnosis of diabetes pathogenesis or its complications. There are also reports regarding an increased level of anti-HSP27 antibodies in atherogenesis. We aimed to evaluate serum anti-heat shock protein 27 antibodies level in subjects with diabetes mellitus and undiagnosed individuals. Materials and Methods: This cross-sectional study was conducted on 6447 MASHAD study subjects, including four groups with diabetes mellitus (n=610), undiagnosed diabetes (n=162), impaired fasting glucose (IFG) (n=619) and normal (n=5056) subjects. Demographic and anthropometric data were obtained from all participants. Fasting serum glucose (FSG) and other parameters were measured. In-house enzyme-linked immune sorbent assay method was used for measuring Anti-HSP27 antibodies levels. Results: There were significant differences in weight (p=0.034), body mass index, ...
Heat shock protein 27 (Hsp27) is over-expressed after cells exposure to stressful conditions that include oxidative stress like diabetes as well as chronic kidney disease. Here, the serum Hsp27 levels in Egyptian type 2 diabetic subjects with and without diabetic nephropathy (DN) were investigated. Serum Hsp27 levels were determined using The AssayMax Human Hsp27 ELISA kit in 72 individuals: 14 diabetic control subjects, 28 diabetic subjects with hypertension and/or dyslipidemia as risk factors for DN and 30 individuals with different DN stages (DN1= 6, DN2= 9 and DN3= 15 patients) according to the estimated glomeular filtration rate. Serum Hsp27 concentrations were significantly higher in patients at risk to and with DN compared to diabetic control subjects (p<0.01). Moreover, before microalbuminuria becomes evident, serum Hsp27 levels showed higher sensitivity and area under the curve compared to common traditional markers for diagnosis of DN as creatinine and microalbuminuria. In conclusion, our results showed, for the first time, that serum Hsp27 concentrations appear to be related to the incidence of DN as a microvascular complication in patients with type 2 diabetes mellitus and we concluded that serum HSP 27 may be used as an early marker for diagnosis of diabetic nephropathy.
Connection between Heat Shock Proteins 60/65 and Diabetic Vascular Complications
Journal of Diabetes & Metabolism, 2013
Introduction: Diabetes mellitus, as a metabolic disease, is closely associated with the development of atherosclerosis lesions. The share of heat shock proteins and antibodies in the HSP-60/65 atherogenesis in patients with type 2 diabetes is still an area subject to numerous studies. Material and methods: The study was conducted in the group of 46 patients with type 2 diabetes mellitus with macrovascular complications and 62 patients with atherosclerosis. The control group was comprised of a cohort of healthy volunteers in the corresponding age. Tissue material acquired during the planned endarterectomy from the internal carotid artery was used to measure the serum levels of anti HSP 60/65, the plasma levels of vWF, and to perform the immunohistochemistry study. Results: There was a higher concentration of anti HSP-60/65 in the group of patients with type 2 diabetes mellitus with macrovascular complications and patients with atherosclerosis without the endocrinopathies than in the control group (55.67 ± 65.43 vs. 26.09 ± 13.85, 82.98 ± 26.09 vs. 111.18 ± 13.85). Immunohistochemical method allowed observing a strong correlation between the concentration of antibodies to HSP60/65 in the blood serum and the protein expression of HSP 60/65 in the carotid arterial wall. The concentration of antibodies to HSP 60/65 was associated with the increase in the concentration of von Willebrand factor-marker of endothelial dysfunction (R=0.388, p<0.01). Conclusions: The research indicated an additional connection between the serum level of antibodies to HSP 60/65 and of vWF, in patients with type 2 diabetic mellitus with macrovascular complications, suggesting their influence on endothelial cell damage in the course of diabetic vascular complications.
SpringerPlus, 2014
Low protein levels of Hsp27 have been reported in atherosclerotic plaques. In addition, human studies have indicated that circulating Hsp27 levels are lower in coronary artery disease patients compared with controls. It remains, however, unclear whether this applies to other forms of atherosclerotic disease. Plasma Hsp27 from 280 subjects was examined by ELISA. The cohort included 80 coronary artery disease (CAD), 40 peripheral artery disease (PAD) and 80 abdominal aortic aneurysm (AAA) patients. Eighty elderly subjects, without any clinical history of vascular diseases, were used as a control group. Receiver operating curve (ROC) and logistic regression model analysis were performed to evaluate the potential value of Hsp27 as a circulating biomarker. Patients with atherosclerotic vascular diseases had significantly lower levels of Hsp27 than control subjects (p < 0.001). Moreover, Hsp27 was significantly lower in CAD patients than other atherosclerotic vascular disease groups (p...
Revista Romana de Medicina de Laborator
Background: Heat shock protein 27 (HSP27) is an intracellular chaperone constitutively expressed in many cell types including cardio myocytes and endothelial cells. Circulating levels of HSP27 and anti-HSP27 antibody are higher in patients with CVD. Anti-HSP27 antibody concentrations were also reported to be increased in atherogenesis. We aimed to evaluate serum anti-HSP27 antibody titers in individuals with, or without, MetS in the MASHAD study cohort with large sample size in 6,568 subjects. Methods: Participants with MetS were identified from MASHAD cohort (n=3358) using the IDF criteria, and the control group were those individuals who did not meet these criteria (n=3210). In-house enzyme-linked immune sorbent assay (ELISA) method was used for measuring Anti-HSP27 antibody levels. The two groups were matched for age, sex and smoking habit. Results: As expected, there were significant differences in height (p= 0.004), waist and hip circumference, weight, BMI, systolic and diastol...