Diagnostic importance of CD179a/b as markers of precursor B-cell lymphoblastic lymphoma (original) (raw)

Abstract

Surrogate light chains consisting of VpreB (CD179a) and k5 (CD179b) are expressed in precursor B cells lacking a complete form of immunoglobulin and are thought to act as substitutes for conventional light chains. Upon differentiation to immature and mature B cells, CD179a/b disappear and are replaced with conventional light chains. Thus, these molecules may be useful as essential markers of precursor B cells. To examine the expression of the surrogate light-chain components CD179a and CD179b in precursor B-cell lymphoblastic lymphoma, we analyzed tissue sections using immunohistochemistry techniques. Among a number of monoclonal antibodies for the surrogate light chains, VpreB8 and SL11 were found to detect CD179a and CD179b, respectively, in acetone-fixed fresh frozen sections. Moreover, we also observed VpreB8 staining in formalin-fixed, paraffin-embedded sections. Using these antibodies, we found that CD179a/b were specifically expressed in precursor B-cell lymphoblastic lymphomas, but not in mature B-cell lymphomas in childhood. Furthermore, other pediatric tumors that must be included in a differential diagnosis of precursor B-cell lymphoblastic lymphoma, including precursor T-cell lymphoblastic lymphoma, extramedullary myeloid tumors, and Ewing sarcoma, were also negative for both CD179a and CD179b. Our data indicate that CD179a and CD179b may be important markers for the immunophenotypic diagnosis of precursor B-cell lymphoblastic lymphomas.

Figures (4)

Figure 1 Immunohistochemical detection of CD179b by HSL11 in acetone-fixed, cytocentrifuged precursor B-ALL cell lines. Pre- BCR-expressing HPB-NULL cells were permeabilized and stained with specific mAbs, as indicated, and analyzed using flow cytometry (left panels). The resulting histograms (solid lines) were superimposed on those of the negative control (cells stained with isotype-matched control mouse Ig, broken light lines) and displayed. X-axis, fluorescence intensity; Y-axis, relative cell number. In parallel, HPB-NULL cells were cytocentrifuged, acetone-fixed, and stained with each mAb, as indicated, using immunohistostaining (right panels). HSL11 is strongly positive and anti-y is moderately positive, but others are negative. Mslg, iso-type matched control mouse immunoglobulin.

Figure 1 Immunohistochemical detection of CD179b by HSL11 in acetone-fixed, cytocentrifuged precursor B-ALL cell lines. Pre- BCR-expressing HPB-NULL cells were permeabilized and stained with specific mAbs, as indicated, and analyzed using flow cytometry (left panels). The resulting histograms (solid lines) were superimposed on those of the negative control (cells stained with isotype-matched control mouse Ig, broken light lines) and displayed. X-axis, fluorescence intensity; Y-axis, relative cell number. In parallel, HPB-NULL cells were cytocentrifuged, acetone-fixed, and stained with each mAb, as indicated, using immunohistostaining (right panels). HSL11 is strongly positive and anti-y is moderately positive, but others are negative. Mslg, iso-type matched control mouse immunoglobulin.

Figure 2 Immunohistochemical detection of CD179a and CD179b. CD179a and CD179b were detected in B-lymphoblastic lymphomé tissues using immunohistochemical staining on acetone-fixed fresh frozen sections ((b), (c), FF) and formalin-fixed, paraffin-embeddec tissue sections ((d), PF) from biopsy tissues. The H&E-staining of formalin-fixed and paraffin-embdded tissues is also shown ((a), HE)

Figure 2 Immunohistochemical detection of CD179a and CD179b. CD179a and CD179b were detected in B-lymphoblastic lymphomé tissues using immunohistochemical staining on acetone-fixed fresh frozen sections ((b), (c), FF) and formalin-fixed, paraffin-embeddec tissue sections ((d), PF) from biopsy tissues. The H&E-staining of formalin-fixed and paraffin-embdded tissues is also shown ((a), HE)

Table 1 Detection of CD179a and CD179b in B-lineage lymphoma tissues using immunohistochemical staining in acetone-fixed fresh frozen sections B-LBL, precursor B-cell lymphoblastic lymphoma; DL, diffuse large cell lymphoma; Bil, bilateral; L, left; R, right; CL, cervical lymph nodes; AT, abdominal tumor; LC, light chains; NT, not tested; P, patchy staining pattern; M, membranous staining pattern. “Testicular relapse of precursor B acute lymphoblastic leukemia.

Table 1 Detection of CD179a and CD179b in B-lineage lymphoma tissues using immunohistochemical staining in acetone-fixed fresh frozen sections B-LBL, precursor B-cell lymphoblastic lymphoma; DL, diffuse large cell lymphoma; Bil, bilateral; L, left; R, right; CL, cervical lymph nodes; AT, abdominal tumor; LC, light chains; NT, not tested; P, patchy staining pattern; M, membranous staining pattern. “Testicular relapse of precursor B acute lymphoblastic leukemia.

Table 2 Immunohistochemical staining of CD179a and CD179b on acetone-fixed fresh frozen sections of non-B-cell lineage neoplasm tissues T-LBL, precursor T-cell lymphoblastic lymphoma; AMoL, acute monocytic leukemia.

Table 2 Immunohistochemical staining of CD179a and CD179b on acetone-fixed fresh frozen sections of non-B-cell lineage neoplasm tissues T-LBL, precursor T-cell lymphoblastic lymphoma; AMoL, acute monocytic leukemia.

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