Age-related changes in normal and cataractous human lens crystallins, separated by fast-performance liquid chromatography (original) (raw)

The study investigates age-related changes in lens crystallins from normal and cataractous human lenses using fast-performance liquid chromatography (FPLC). Analysis of crystallins from 32 normal and 18 cataractous lenses reveals an increase in aH-crystallin and a decrease in aL-crystallin with aging, while low-molecular-weight crystallins remain largely unchanged. Differences in chromatographic profiles between diabetic and nondiabetic cataracts suggest that cataractogenesis in diabetes may involve distinct mechanisms that augment the normal aging process.