Thein vitro effect of aqueous extract ofNigella sativa seeds on nitric oxide production (original) (raw)
Related papers
2014
Nigella sativa (NS) Linn. (Ranunculaceae) oil is used in Northern Nigeria for the treatment of malaria, stomach ulcer, and other inflammatory diseases. The effect of NS (NS) oil on inflammation and the nitric oxide (NO) system was investigated in this study. Chronic inflammation was induced using formaldehyde arthritic model while acute inflammation was produced using carrageenan-induced paw oedema in rats. For the chronic inflammatory model, the animals were divided into four groups of five animals each as follows: Group A, B, C, and D, they were administered normal saline, indomethacin (5 mg/Kg), NS oil (1 ml/Kg), and NS oil (2 ml/Kg) orally respectively, while the acute inflammatory model had a fifth group, E, administered N G -nitro-L-arginine methyl ester (L-NAME, 100 mg/Kg). In order to investigate the effect of L-arginine administration, the acute inflammation experiment was repeated with intraperitoneal administration of L arginine (300 mg/kg) to all groups 1h after the indu...
International Journal of Pharmacy and Pharmaceutical Sciences
Objective: Nitric oxide (NO) is a signaling molecule that plays a key role in the pathogenesis of inflammation. Inhibitors of NO may be useful candidates for the treatment of inflammatory diseases. The study aimed to determine the antioxidant and inhibitory effect of commonly used Ghanaian vegetables, namely Corchorus olitorius (CO), Solanum melongena (SM), Solanum torvum (ST), Xanthosoma sagittifolia (XS) and Abelmoschus esculentus (AE) on NO expression in a Lipopolysaccharide (LPS)-induced RAW 264.7 macrophage cell line. Methods: The cytotoxic effects of the vegetables on the cell line were determined using a tetrazolium-based colorimetric assay. The inflammatory activity was determined by measuring the inhibition of NO production in LPS-induced RAW 264.7 macrophage cells. Total antioxidant activity, total phenolic, flavonoid, and reduced glutathione contents were evaluated using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, Folin-Ciocalteu, aluminium chloride, and O-Phthalaldehyde...
Indian Journal of Experimental Biology, 2012
There is general belief that only pure phytomolecules may be used as molecular therapeutic agent through one to one action. However, the traditional systems of medicine e.g. Ayurveda, uses the crude extracts, mostly water decoctions and oils, as drug. A comparative study of hexane, ethyl acetate and methanol fractions of N. Sativa seeds has been carried out on fresh rat-peritoneal-macrophage culture with reference to their role on various targets of lipopolysaccharide induced release of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) expression. The results indicated significant antioxidant potential with methanolic extract as most effective. Its mechanism of action was proposed primarily through its antioxidant potential and not through direct inhibition of other kinases, involved in its signaling cascade.
Evaluation of anti-inflammatory activity of Nigella sativa: An experimental study
National Journal of Physiology, Pharmacy and Pharmacology, 2017
Background: Long-term use of drugs currently used for the treatment of inflammation is associated with serious adverse effects. Nigella sativa, an herb exclusively used in traditional medicine, is reported to inhibit both cyclooxygenase (COX) and 5-lipoxygenase pathways of arachidonic acid metabolism. Aims and Objectives: To evaluate of antiinflammatory activity N. sativa seed fixed oil in different models of inflammation in rats and to compare it with control and aspirin. Materials and Methods: Albino Wistar rats of either sex weighing 180-200 g were used in this study. N. sativa seed fixed oil was used to evaluate anti-inflammatory by carrageenin-induced hind paw edema, cotton pellet granuloma, and formaldehyde induced arthritis method by oral administration in healthy albino rats. The study was conducted with prior approval of Institutional Animal Ethics Committee. Results: In the model of acute inflammation, i.e., carrageenin-induced paw edema in rats, N. sativa showed anti-inflammatory activity which was statistically significant as compared to control (P < 0.001) but less than aspirin. In cotton pellet induced granuloma method, N. sativa significantly decreased the formation of granulomatous tissue, as compared to control (P < 0.001). N. sativa showed significant anti-inflammatory activity comparable to aspirin in formaldehyde induced arthritis model of chronic inflammation (P > 0.05). Conclusion: The result of this study suggests that N. sativa seed fixed oil possesses significant anti-inflammatory activity in rats.
Biological and Pharmaceutical Bulletin, 2004
In an attempt to search for bioactive natural products exerting antiinflammatory activity, we have evaluated the effects of the methanol extract of the fruits of Kochia scoparia (L.) CHARD. (Chenopodiaceae) on lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E 2 (PGE 2), and tumor necrosis factor (TNF)-a a release by the macrophage cell line RAW 264.7. Our data indicate that this extract is a potent inhibitor of NO production and it also significantly decreased PGE 2 and TNF-a a release. Consistent with these observations, the protein and mRNA expression level of inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 was inhibited by MeOH extracts of Kochia scoparia (KSM) in a dose-dependent manner. Furthermore, KSM inhibited the LPS-induced DNA binding activity of nuclear factor-k kB (NF-k kB), which was associated with prevention of the inhibitor k kB degradation. These results suggest that the methanol extract of K. scoparia inhibits LPS-induced iNOS and COX-2 expression by blocking NF-k kB activation.
Journal of Food Biochemistry, 2011
Nigella sativa is a one of the most commonly found aromatics in the Mediterranean kitchen. This plant is traditionally used for the treatment of several pathologies. In this study, the antioxidant, anti-inflammatory, anticancer and antibacterial activities of the shoots, roots and seeds methanol extracts from N. sativa were studied. The three organs exhibited strong antioxidant activity using the oxygen radical absorbance capacity method and a cell-based assay. Furthermore, the seeds hexane fraction (SHF) of the methanol extract showed significant anti-inflammatory activity, inhibiting nitric oxide release with an IC50 value of 6.20 mg/mL in lipopolysaccharide-stimulated RAW 264.7 macrophages. The SHF was found to be active against A-549 lung carcinoma cells and DLD-1 colon carcinoma, with IC50 values of 31.0 and 63.0 mg/mL, respectively, as well as against Staphylococcus aureus (ATCC 25923) and Escherichia coli (ATCC 25922) bacteria with IC50 values of 38.0 and 80.0 mg/mL, respectively. The shoots methanol extract exhibited moderate antiinflammatory activity but no anticancer and antibacterial activities. PRACTICAL APPLICATIONS The results obtained suggest that Nigella sativa plant parts, including the shoots, the roots and the seeds, have the potential to be developed into dietary supplements as food preservative and for the improvement of human nutrition and health. In addition, the seeds could be useful for therapeutical purposes and could be developed as anticancer agent and as foodborne preservative as well as for the treatment of chronic inflammatory pathologies associated with overproduction of nitric oxide.
Nitric oxide inhibitory activity of herbal extract formulae for anti-inflammation
Thammasat Medical Journal, 2014
Introduction: The objective of this research was to investigate anti-inflammatory activity of herbal extracts formulae from four plants, Zingiber cassumunar (ZC), Centella asiatica (CA), Zingiber officinale (ZO) and Piper nigrum (PN) by variable proportion of each plant. The active compound(s) contributing to its anti-inflammatory activity of best formula was verified using high performance liquid chromatography (HPLC) method. Method: Inhibitory activity against lipopolysaccharide (LPS) induced nitric oxide production in RAW 264.7 cell lines was used as assay for anti-inflammation. Each plant and five formulae (CF1-CF5) were extracted by maceration in 95% ethanol. Result: The ethanolic extract of CF2 and CF4 showed the highest nitric oxide inhibitory activities with IC 50 value of 7.83 and 7.87 µg/ml, respectively. Three plant extracts, PN, ZO and ZC also exhibited high nitric oxide inhibitory activities with IC 50 value of 10.52, 11.93 and 21.33 µg/ml, respectively. The studies on chemical constituents using high performance liquid chromatography (HPLC) showed that piperine and (E)-1-(3,4-dimethoxyphenyl) butadiene (DMPBD) were a major compound in the combine formula 2 and 4 extracts. Terpinine-4-ol, 6-gingerol and asiatic acid were a minor compound in the extracts. Discussion and Combination of herbal extract such as CF2 and CF4 formulae possessed good nitric oxide inhibitory Conclusion: activity which was higher than single plant extract.
Natural Product Research, 2019
The WSE is a highly polar, gummy and mucilaginous bioactive content of the of Nigella sativa (L.) seeds. This study reports the anxiolytic and anti-inflammatory effects of WSE investigated using Elevated Plus Maze (EPM) and Hole-Board Test (HBT) in adult mice and human RBCs haemolysis inhibition and protein denaturation respectively. The oral WSE treatment (100 & 200 mg/kg b.w/day) for 72 hours has exhibited slightly better anxiolytic effect (p < 0.05) by the time span (92.33 & 93.33s) spent in the opened arms of EPM vs. diazepam (1 mg/kg b.w i.p/day; 69.33s). In HBT, only WSE (200 mg/kg b.w/day) has shown a promising number of mean head pokes (13.27 times/min) vs. diazepam (12.87 times/min). The oral WSE (62.5-500 µg/mL) has exhibited 40.14-72.18% protection against lysis of RBCs vs. aspirin (57.04-71.48%) whilst 62.67-67.66% inhibition of protein denaturation vs. diclofenac sodium (43.11-80.64%). The current findings suggested WSE has promising anxiolytic and anti-inflammatory activities.
In vitro Effects of Nigella sativa Seeds Extracts on Stimulated Peripheral Blood Mononuclear Cells
pteridines, 2008
Nigella sativa, commonly known as black cumin seed, belongs to the botanical family of Ranunculaceae. The active antioxidant components of Nigella sativa display a remarkable array of biochemical, immunological and pharmacological actions, including bronchodilatory, anti-inflammatory, antibacterial, hypoglycaemic, antitumoural and immunopotentiating effects. Effects of Nigella sativa seeds extracts were investigated in freshly isolated human peripheral blood mononuclear cells stimulated with the mitogens phytohaemagglutinin and concanavalin A in vitro. Tryptophan degradation and neopterin production were monitored in culture supernatants, both these immunobiochemical pathways are induced by pro-inflammatory cytokine interferon-γ. Compared to unstimulated cells, the mitogens enhanced degradation of tryptophan and production of neopterin. Nigella sativa seeds extracts significantly suppressed both pathways in a dose-dependent way. Suppression of tryptophan degradation and neopterin fo...
Enhancement in anti-inflammatory and analgesic activities of Nigella sativa seed during germination
Pharmacognosy Journal, 2014
Objective: The medicinal values of Nigella sativa have been mentioned in ancient literature as useful in disorders of inflammation. The present study investigates the anti-inflammatory and analgesic activities of methanol extract of Nigella sativa seed during different phases of its germination on Wistar rats. Methods: Seeds of N. sativa were grown in vitro in glass petri plates using multiple folds of damp filter paper. Complete plantlet with two leaves was obtained in 11 days. The acute anti-inflammatory activity of N. sativa extracts during different phases of germination was measured plethysmographically using kaolin as inflammatory agent, analgesic activity was measured by hot plate method, keeping indomethacin (10mg/kg b.w) as reference standard in both tests.