Effects of metoclopramide and ranitidine on survival of flat template McFarlane skin flaps in a rat wound healing model (original) (raw)
Related papers
Histological Study of the First Seven Days of Skin Wound Healing in Rats
Acta Veterinaria Brno, 2006
The aim of this study was to elaborate a histological model of incisional skin wound healing in Sprague-Dawley rats. Under aseptic conditions two paravertebral full thickness skin incisions were performed on the back of 42 anesthetized male rats. Histological sections from tissue specimens were stained by hematoxylin and eosin, van Gieson, PAS + PSD, Mallory's phosphotungstic hematoxylin and azur and eosin and evaluated during the first seven days after surgery. Histological evaluation revealed that the regeneration of injured epidermis was completed five days after surgery. The inflammatory phase was recorded during the first three days of healing with the culmination of this phase between day one and day two. The beginning of the proliferative phase was dated to the first day and the peak during day five and day six. The initiation of the maturation and remodeling phase of the healing process was observed six days after wounding. At the layer of striated muscle, the centronucl...
Description of a new experimental model skin flap for studying skin viability in rats
Acta cirúrgica brasileira / Sociedade Brasileira para Desenvolvimento Pesquisa em Cirurgia, 2014
To describe a new experimental flap for studying skin viability in rats. Twenty male Wistar rats weighing between 250-300g were divided into two groups: group A - McFarlane technique, a 4 x 10cm flap was used (McFarlane); and in group B modified McFarlane technique, a 3 x 10cm flap was used. Seven days later, the animals were sacrificed and the area of necrosis was evaluated in both groups. Group A presented necrosis in 3% of the total area of the flap (CI: 0.01-0.05), Group B presented necrosis in 37% of the total area of the flap (CI: 0.29-0.46), (p<0.001). The modified McFarlane flap presented a larger area of necrosis and could be an adequate experimental model of skin flap viability.
Models of Rat Skin Wound Healing: An Overview
Asian Journal of Pharmaceutical and Clinical Research
Wounds have developed into one of the earliest types of human pain, with two histories that go back to before the existence of humans. The growth of medical research led to an abundance of new ideas and opened the doors for creating a separate field solely dedicated to treating wounds. The underlying cause, the site of the injury, the mechanism of injury-producing symptoms, the depth and tissue loss of the wound, or the clinical presentation can all be used to categorize wounds. According to studies utilizing animal models, there are four stages of acute wound healing. It is a given that chronic wounds must go through similar underlying mechanisms. Hemostasis, inflammation, proliferation or granulation, and remodeling or maturation are adequate stages of wound healing. There has been a substantial change in how we understand and apply information. This study investigated every aspect of wound healing, including every pathway and model for wound healing.
Effect of Topically Applied Minoxidil on the Survival of Rat Dorsal Skin Flap
Aesthetic Plastic Surgery, 2012
Background Flap necrosis still is a challenging problem in reconstructive surgery that results in irreversible tissue loss. This study evaluated the effect of topically applied minoxidil on angiogenesis and survival of a caudally based dorsal rat skin flap. Methods For this study, 24 male Wistar rats were randomly divided into three groups of eight each. A caudally based dorsal skin flap with the dimensions of 9 9 3 cm was raised. After elevation of the flaps, they were sutured back into their initial positions. In group 1 (control group), 1 ml of isotonic saline was applied topically to the flaps of all the animals for 14 days. In group 2, minoxidil solution was spread uniformly over the flap surface for 7 days after the flap elevation. In group 3, minoxidil solution was applied topically to the flap surface during a 14-day period. On day 7 after the flap elevation, the rats were killed. The average area of flap survival was determined for each rat. Subdermal vascular architecture and angiogenesis were evaluated under a light microscope after two full-thickness skin biopsy specimens had been obtained from the midline of the flaps. Results The lowest flap survival rate was observed in group 1, and no difference was observed between groups 1 and 2. Compared with groups 1 and 2, group 3 had a significantly increased percentage of flap survival (P \ 0.05). Intense and moderate angiogenesis also was observed respectively at the proximal and distal areas of the flaps in group 3.
Journal of Visualized Experiments, 2013
Wound healing and repair are the most complex biological processes that occur in human life. After injury, multiple biological pathways become activated. Impaired wound healing, which occurs in diabetic patients for example, can lead to severe unfavorable outcomes such as amputation. There is, therefore, an increasing impetus to develop novel agents that promote wound repair. The testing of these has been limited to large animal models such as swine, which are often impractical. Mice represent the ideal preclinical model, as they are economical and amenable to genetic manipulation, which allows for mechanistic investigation. However, wound healing in a mouse is fundamentally different to that of humans as it primarily occurs via contraction. Our murine model overcomes this by incorporating a splint around the wound. By splinting the wound, the repair process is then dependent on epithelialization, cellular proliferation and angiogenesis, which closely mirror the biological processes of human wound healing. Whilst requiring consistency and care, this murine model does not involve complicated surgical techniques and allows for the robust testing of promising agents that may, for example, promote angiogenesis or inhibit inflammation. Furthermore, each mouse acts as its own control as two wounds are prepared, enabling the application of both the test compound and the vehicle control on the same animal. In conclusion, we demonstrate a practical, easy-to-learn, and robust model of wound healing, which is comparable to that of humans.
Aims: This study attempts to investigate variations in skin and wound healing, by comparatively examining the rate of healing between Sprague Dawley rats (Rattus Norveigicus) and Brazilian agouti (Dasyprocta Leporina), reflective of two skin types. Materials and Methods: Following the excision wound model, wound healing times and dimensions between the two species, were compared. Each species was divided into two groups of 3. The standard/experimental group was treated with mupirocin ointment and the control group was left untreated. Results: In comparing both species, on day 15 the rats exhibited a greater reduction in wound area (92%), as compared to the agouti (67%). The histological study of the granulation tissue from rats showed well developed collagen than the granulation tissue obtained from agouti. Conclusions: The results of our study demonstrated that the thick and hard skin takes more time to heal than the skin with smooth/thinner texture.
Delayed cutaneous wound healing in aged rats compared to younger ones
International Wound Journal, 2012
Delayed wound healing in elderly males is a complex process in which the factors responsible are not fully understood. This study investigated the hormonal, oxidative and angiogenic factors affecting wound healing in aged rats. Two groups consisting of eight healthy male Wistar Albino rats [young (30 ± 7 days) and aged (360 ± 30 days)], and a cutaneous incision wound healing model were used. Scar tissue samples from wounds on the 7th, 14th and 21st days of healing were evaluated for hydroxyproline and vascular endothelial growth factor content. Macrophage, lymphocyte, fibroblast and polymorphonuclear cell infiltration; collagen formation and vascularization were assessed by light and electron microscopy. The free oxygen radical content of the wounds was measured by a chemiluminescence method. Blood sample analysis showed that the hydroxyproline and total testosterone levels were significantly higher, and the oxygen radical content was significantly lower in young rats. Histopathological, immunohistochemical and ultrastructural evaluations revealed higher amounts of fibroblasts and collagen fibers, and more vascularization in young rats. These results are indicative of the delayed wound healing in aged rats. A combination of multiple factors including hormonal regulation, free oxygen radicals and impaired angiogenesis appears to be the cause of delayed cutaneous healing.
Veterinární Medicína
The use of a simple and reproducible model is inevitable for objective statement of the effects of external factors on wound healing. Hence, present study was conducted to establish an excisional model of skin wound healing in corticosteroid treated, and streptozotocine induced diabetic rats as well as to standardized the semi-quantitative and quantitative evaluation of selected parameters. Round full thickness skin wounds were performed on the back of male Sprague-Dawley rats. Animals were sacrificed two, six, and fourteen days after surgery. Sections were stained with hematoxylin-eosin and van Gieson. Both semi-quantitative (wound reepithelization; presence of: inflammatory cells, fibroblasts, new wessels, and collagen) and quantitative methods (polymorphonuclear leucocytes/tissue macrophages ratio, percentage of re-epithelization, area of the granulation tissue) were used to evaluate the histological changes during wound healing. As compared to the control group the wound healing...