Cytokeratin 5-Positive Cells Represent a Therapy Resistant subpopulation in Epithelial Ovarian Cancer (original) (raw)

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, 2015

Abstract

Cytokeratin 5 (CK5) is an epithelial cell marker implicated in stem and progenitor cell activity in glandular reproductive tissues and endocrine and chemotherapy resistance in estrogen receptor (ER) breast cancer. The goal of this study was to determine the prevalence of CK5 expression in ovarian cancer and the response of CK5 cell populations to cisplatin therapy. Cytokeratin 5 expression was evaluated in 2 ovarian tissue microarrays, representing 137 neoplasms, and 6 ovarian cancer cell lines. Cell lines were treated with IC50 (half-maximal inhibitory concentration) cisplatin, and the prevalence of CK5 cells pretreatment and posttreatment was determined. Proliferation of CK5 versus CK5 cell populations was determined using 5-bromo-2'-deoxyuridine incorporation. Chemotherapy-induced apoptosis in CK5 versus CK5 cells was measured using immunohistochemical staining for cleaved caspase-3. Cytokeratin 5 was expressed in 39.3% (42 of 107) of epithelial ovarian cancers with a range o...

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