Experimental and theoretical evaluation of surface plasmon-coupled emission for sensitive fluorescence detection (original) (raw)

2008, Journal of Biomedical Optics

Surface plasmon-coupled emission SPCE is a phenom- enon whereby the light emitted from a fluorescent molecule can couple into the surface plasmon of an adjacent metal layer, resulting in highly directional emission in the region of the surface plasmon resonance SPR angle. In addition to high directionality of emission, SPCE has the added advantage of surface selectivity in that the

Plasmon-controlled fluorescence towards high-sensitivity optical sensing

Advances in biochemical engineering/biotechnology, 2009

Fluorescence spectroscopy is widely used in chemical and biological research. Until recently most of the fluorescence experiments have been performed in the far-field regime. By far-field we imply at least several wavelengths from the fluorescent probe molecule. In recent years there has been growing interest in the interactions of fluorophores with metallic surfaces or particles. Near-field interactions are those occurring within a wavelength distance of an excited fluorophore. The spectral properties of fluorophores can dramatically be altered by near-field interactions with the electron clouds present in metals. These interactions modify the emission in ways not seen in classical fluorescence experiments. Fluorophores in the excited state can create plasmons that radiate into the far-field and fluorophores in the ground state can interact with and be excited by surface plasmons. These reciprocal interactions suggest that the novel optical absorption and scattering properties of m...

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